MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1334 857.4230 856.4157
1335 857.9269 856.9196
1338 859.3800 858.3727
1339 859.4172 858.4099
1340 859.4236 858.4163
1343 861.4099 860.4026
1345 861.4247 860.4174
1347 862.4238 861.4165
1348 862.4418 861.4345
1349 863.4089 862.4016
1350 432.2405 862.4664
1351 864.4321 863.4248
1352 864.4744 863.4671
1353 865.4170 864.4097
1354 866.4128 865.4055
1355 866.4138 865.4065
1356 867.3856 866.3783
1357 867.3859 866.3786
1358 434.1966 866.3786
1359 867.4178 866.4105
1360 867.4225 866.4152
1361 868.4229 867.4156
1362 434.7675 867.5204
1364 870.4157 869.4084
1365 870.4216 869.4143
1366 870.4250 869.4177
1367 435.7754 869.5362
1368 871.4059 870.3986
1369 871.4197 870.4124
1370 871.4219 870.4146
1371 872.0877 871.0804
1372 872.4110 871.4037
1373 872.4326 871.4253
1374 436.7295 871.4444
1375 874.4266 873.4193
1376 874.4324 873.4251
1377 875.4107 874.4034
1378 875.4252 874.4179
1379 876.4205 875.4132
1382 877.4157 876.4084
1383 439.7283 877.4420
1384 439.7284 877.4422
1386 879.4250 878.4177
1388 440.2581 878.5016
1389 881.4248 880.4175
1390 441.7022 881.3898
1391 882.4028 881.3955
1392 882.4379 881.4306
1393 441.7381 881.4616
1394 882.4691 881.4618
1395 883.3832 882.3759
1397 884.4298 883.4225
1398 884.4333 883.4260
1400 886.4246 885.4173
1402 888.4371 887.4298
1403 889.4241 888.4168
1404 889.4319 888.4246
1405 446.1751 890.3356
1406 891.4301 890.4228
1407 892.4231 891.4158
1408 892.4379 891.4306
1409 892.4415 891.4342
1410 892.4430 891.4357
1411 892.4462 891.4389
1414 446.7520 891.4894
1415 893.4595 892.4522
1416 447.2663 892.5180
1418 448.2374 894.4602
1420 896.4463 895.4390
1421 897.4307 896.4234
1423 449.6994 897.3842
1424 898.4270 897.4197
1425 898.4301 897.4228
1426 898.4317 897.4244
1427 898.4327 897.4254
1429 899.3754 898.3681
1430 450.2342 898.4538
1432 450.2516 898.4886
1433 450.2524 898.4902
1435 900.4346 899.4273
1436 900.4398 899.4325
1437 450.7247 899.4348
1438 900.4506 899.4433
1439 902.4517 901.4444
1441 903.4263 902.4190
1442 903.4397 902.4324
1443 904.4364 903.4291
1444 453.1699 904.3252
1445 905.4418 904.4345
1446 905.4433 904.4360
1447 905.4755 904.4682
1450 453.7579 905.5012
1451 907.4243 906.4170
1453 909.4332 908.4259
1454 910.4249 909.4176
1455 910.4352 909.4279
1456 910.4406 909.4333
1457 910.4529 909.4456
1458 911.4324 910.4251
1459 911.4428 910.4355
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