MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1194 781.3727 780.3654
1195 391.2150 780.4154
1197 391.2206 780.4266
1199 392.2222 782.4298
1200 392.2222 782.4298
1201 783.4375 782.4302
1202 784.3829 783.3756
1203 784.3991 783.3918
1204 787.3943 786.3870
1205 787.3943 786.3870
1206 787.4221 786.4148
1207 793.3984 792.3911
1210 793.9261 792.9188
1214 397.6886 793.3626
1216 795.4119 794.4046
1219 795.9143 794.9070
1220 797.3801 796.3728
1221 797.3802 796.3729
1222 399.1941 796.3736
1223 798.3792 797.3719
1224 798.3974 797.3901
1225 400.2198 798.4250
1229 800.3948 799.3875
1232 800.4280 799.4207
1233 801.4263 800.4190
1234 802.4321 801.4248
1235 802.8823 801.8750
1236 805.3948 804.3875
1237 805.4051 804.3978
1238 805.4057 804.3984
1239 805.4284 804.4211
1244 810.3972 809.3899
1245 811.4169 810.4096
1247 406.2257 810.4368
1248 813.3741 812.3668
1249 813.4100 812.4027
1250 814.3932 813.3859
1251 814.3978 813.3905
1253 408.6796 815.3446
1254 816.4244 815.4171
1255 817.4230 816.4157
1256 818.4086 817.4013
1257 818.4106 817.4033
1260 411.2180 820.4214
1261 411.2362 820.4578
1262 822.3731 821.3658
1263 822.4095 821.4022
1265 412.6731 823.3316
1266 412.7544 823.4942
1267 824.9175 823.9102
1268 825.4101 824.4028
1269 413.2091 824.4036
1270 826.3972 825.3899
1272 828.3738 827.3665
1273 828.4004 827.3931
1274 828.4456 827.4383
1276 831.4065 830.3992
1277 416.6617 831.3088
1278 832.3946 831.3873
1279 832.4056 831.3983
1280 833.8965 832.8892
1281 834.3828 833.3755
1282 834.3862 833.3789
1283 834.4044 833.3971
1284 835.4036 834.3963
1285 835.4082 834.4009
1287 836.4196 835.4123
1288 836.4383 835.4310
1289 418.7232 835.4318
1290 418.7234 835.4322
1291 419.2054 836.3962
1292 840.4058 839.3985
1293 840.4150 839.4077
1296 840.8784 839.8711
1297 841.3694 840.3621
1298 841.3699 840.3626
1299 421.1890 840.3634
1301 842.4024 841.3951
1302 842.4065 841.3992
1303 421.7229 841.4312
1305 842.5123 841.5050
1308 843.4146 842.4073
1309 422.2134 842.4122
1310 844.4300 843.4227
1312 845.4142 844.4069
1315 423.2315 844.4484
1316 846.4223 845.4150
1317 846.9267 845.9194
1318 848.4200 847.4127
1319 848.4214 847.4141
1321 851.4033 850.3960
1322 851.4105 850.4032
1323 852.4227 851.4154
1327 427.2493 852.4840
1328 854.4095 853.4022
1329 427.7107 853.4068
1330 855.3847 854.3774
1331 855.4061 854.3988
1332 428.7309 855.4472
1333 857.4026 856.3953
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