MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1041 329.1824 656.3502
1043 660.3175 659.3102
1044 664.9766 663.9693
1045 665.3630 664.3557
1046 333.1907 664.3668
1047 665.3745 664.3672
1048 333.2032 664.3918
1049 334.1803 666.3460
1052 334.7033 667.3920
1053 669.8561 668.8488
1054 670.3293 669.3220
1055 671.2997 670.2924
1056 671.3010 670.2937
1062 675.4191 674.4118
1066 681.3563 680.3490
1067 681.3689 680.3616
1068 341.1887 680.3628
1069 341.2005 680.3864
1070 341.2005 680.3864
1071 341.2008 680.3870
1072 681.3997 680.3924
1073 681.8140 680.8067
1074 682.3304 681.3231
1075 682.3351 681.3278
1076 683.3760 682.3687
1077 683.3834 682.3761
1079 683.8441 682.8368
1080 685.3531 684.3458
1081 343.1980 684.3814
1082 345.1778 688.3410
1083 690.3505 689.3432
1084 695.3457 694.3384
1085 348.2083 694.4020
1086 696.3322 695.3249
1087 697.3145 696.3072
1092 701.3644 700.3571
1093 703.3348 702.3275
1098 708.3387 707.3314
1100 354.6939 707.3732
1101 711.4189 710.4116
1102 712.3274 711.3201
1103 712.3637 711.3564
1104 713.3465 712.3392
1105 717.3438 716.3365
1106 718.6584 717.6511
1107 718.9940 717.9867
1108 361.6999 721.3852
1111 724.0259 723.0186
1112 724.4115 723.4042
1113 362.7094 723.4042
1114 725.3727 724.3654
1117 726.3412 725.3339
1118 726.3432 725.3359
1119 728.3987 727.3914
1121 365.7267 729.4388
1124 732.3617 731.3544
1125 367.1910 732.3674
1126 367.1912 732.3678
1128 737.8521 736.8448
1129 738.3470 737.3397
1130 738.3922 737.3849
1131 739.8519 738.8446
1132 740.3588 739.3515
1136 742.3367 741.3294
1139 743.3563 742.3490
1140 743.3741 742.3668
1141 743.3981 742.3908
1145 744.3544 743.3471
1147 746.4075 745.4002
1148 374.1712 746.3278
1149 748.3185 747.3112
1151 752.3620 751.3547
1152 756.3536 755.3463
1153 756.3544 755.3471
1154 756.3931 755.3858
1155 757.4159 756.4086
1156 757.4183 756.4110
1157 379.2174 756.4202
1158 379.6941 757.3736
1161 380.2304 758.4462
1162 380.2305 758.4464
1163 380.2311 758.4476
1164 380.2311 758.4476
1165 380.2313 758.4480
1166 380.2314 758.4482
1167 380.2318 758.4490
1168 380.2321 758.4496
1171 762.3564 761.3491
1172 764.4341 763.4268
1174 767.3696 766.3623
1175 767.3870 766.3797
1178 770.3842 769.3769
1179 770.3848 769.3775
1182 772.3486 771.3413
1184 775.3786 774.3713
1185 775.8751 774.8678
1186 389.6908 777.3670
1189 778.3767 777.3694
1191 390.2123 778.4100
1192 390.2127 778.4108
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