MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
909 457.2291 456.2218
910 457.2296 456.2223
911 457.2299 456.2226
912 458.2372 457.2299
913 459.1028 458.0955
914 459.2205 458.2132
915 459.2414 458.2341
916 461.1188 460.1115
917 461.2572 460.2499
918 461.2582 460.2509
919 461.2585 460.2512
920 461.2587 460.2514
921 466.2432 465.2359
922 468.2937 467.2864
923 472.2786 471.2713
924 473.7359 472.7286
925 474.2296 473.2223
926 474.2315 473.2242
927 474.2318 473.2245
928 475.2400 474.2327
929 476.2365 475.2292
930 477.1133 476.1060
931 488.1935 487.1862
932 488.2359 487.2286
934 493.0198 492.0125
935 493.0218 492.0145
936 493.0218 492.0145
937 493.0219 492.0146
938 493.0220 492.0147
939 493.0223 492.0150
940 493.0226 492.0153
941 493.0230 492.0157
942 493.0230 492.0157
943 493.0235 492.0162
944 493.0240 492.0167
945 494.8161 493.8088
947 511.2808 510.2735
948 513.3157 512.3084
950 527.2831 526.2758
951 529.2631 528.2558
954 535.2505 534.2432
955 539.3215 538.3142
956 542.2057 541.1984
958 544.7740 543.7667
959 545.2573 544.2500
960 545.2780 544.2707
963 556.3106 555.3033
964 562.2739 561.2666
965 570.7175 569.7102
966 570.7178 569.7105
967 570.7185 569.7112
968 570.7189 569.7116
969 570.7191 569.7118
970 570.7194 569.7121
971 570.7196 569.7123
972 571.3118 570.3045
973 573.2897 572.2824
975 574.9587 573.9514
976 576.3543 575.3470
977 579.3159 578.3086
978 580.2998 579.2925
979 582.2896 581.2823
982 586.2857 585.2784
984 592.2723 591.2650
985 596.3431 595.3358
986 602.2797 601.2724
988 303.8864 605.7582
989 303.8869 605.7592
990 303.8873 605.7600
991 608.3408 607.3335
993 304.8365 607.6584
994 304.8365 607.6584
995 304.8368 607.6590
996 304.8368 607.6590
997 304.8368 607.6590
998 304.8369 607.6592
999 612.3005 611.2932
1000 612.3365 611.3292
1007 621.2979 620.2906
1009 624.3361 623.3288
1010 624.3366 623.3293
1012 314.6748 627.3350
1013 629.2900 628.2827
1016 317.6637 633.3128
1017 634.3219 633.3146
1019 635.3057 634.2984
1020 636.3831 635.3758
1023 639.3157 638.3084
1024 320.1773 638.3400
1025 321.1850 640.3554
1026 644.3280 643.3207
1028 644.3372 643.3299
1031 648.3740 647.3667
1032 325.3500 648.6854
1033 651.3587 650.3514
1034 327.1908 652.3670
1035 655.3440 654.3367
1036 328.1927 654.3708
1037 328.1928 654.3710
1039 656.8186 655.8113
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