MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
809 423.2220 422.2147
810 423.2226 422.2153
811 423.2230 422.2157
812 423.2233 422.2160
813 423.2234 422.2161
814 423.2235 422.2162
815 423.2236 422.2163
816 423.2236 422.2163
817 423.2911 422.2838
818 425.2348 424.2275
819 425.2365 424.2292
820 425.2368 424.2295
821 425.2368 424.2295
822 425.2370 424.2297
823 425.2372 424.2299
824 425.2376 424.2303
825 425.2897 424.2824
826 425.2899 424.2826
827 425.2905 424.2832
828 425.2918 424.2845
829 426.2078 425.2005
830 426.2406 425.2333
831 427.2537 426.2464
832 427.2540 426.2467
833 427.2541 426.2468
834 427.2549 426.2476
835 428.2543 427.2470
836 428.2554 427.2481
837 428.2556 427.2483
838 428.2559 427.2486
839 428.2560 427.2487
840 428.2560 427.2487
841 428.2563 427.2490
842 430.2308 429.2235
843 430.3005 429.2932
844 430.3025 429.2952
845 430.3027 429.2954
846 437.1969 436.1896
847 437.2005 436.1932
848 437.2008 436.1935
849 437.2015 436.1942
850 437.2020 436.1947
851 437.2021 436.1948
852 437.2022 436.1949
853 438.1987 437.1914
854 439.2150 438.2077
855 439.2165 438.2092
856 439.2165 438.2092
857 439.2166 438.2093
858 439.2167 438.2094
859 439.2168 438.2095
860 439.2170 438.2097
861 439.2175 438.2102
862 439.2177 438.2104
863 439.2184 438.2111
864 439.2185 438.2112
865 439.2187 438.2114
866 439.2189 438.2116
867 440.2877 439.2804
868 441.2099 440.2026
869 441.2113 440.2040
870 441.2116 440.2043
871 441.2308 440.2235
872 441.2321 440.2248
873 441.2322 440.2249
874 441.2345 440.2272
875 442.2419 441.2346
876 443.2463 442.2390
877 443.2476 442.2403
878 443.2477 442.2404
879 443.2478 442.2405
880 443.2478 442.2405
881 443.2484 442.2411
882 443.2492 442.2419
883 443.2493 442.2420
884 443.2494 442.2421
885 443.2497 442.2424
886 444.2830 443.2757
887 446.2253 445.2180
888 446.2617 445.2544
889 446.7364 445.7291
890 447.2779 446.2706
891 449.1513 448.1440
892 451.2507 450.2434
893 455.2109 454.2036
894 455.2119 454.2046
895 455.2120 454.2047
896 455.2127 454.2054
897 455.2128 454.2055
898 455.2149 454.2076
899 455.2721 454.2648
900 457.2253 456.2180
901 457.2269 456.2196
902 457.2271 456.2198
903 457.2272 456.2199
904 457.2274 456.2201
905 457.2275 456.2202
906 457.2282 456.2209
907 457.2283 456.2210
908 457.2285 456.2212
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