MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
709 395.2269 394.2196
710 395.2614 394.2541
711 395.2623 394.2550
712 395.2623 394.2550
713 396.2656 395.2583
714 397.2050 396.1977
715 397.2060 396.1987
716 397.2061 396.1988
717 397.2061 396.1988
718 397.2061 396.1988
719 397.2069 396.1996
720 397.2070 396.1997
721 397.2071 396.1998
722 397.2075 396.2002
723 397.2078 396.2005
724 398.2400 397.2327
725 398.2793 397.2720
726 399.2207 398.2134
727 399.2227 398.2154
728 399.2228 398.2155
729 399.2228 398.2155
730 399.2234 398.2161
731 399.2234 398.2161
732 399.2236 398.2163
733 399.2237 398.2164
734 400.2322 399.2249
735 400.2323 399.2250
736 401.2366 400.2293
737 402.2467 401.2394
738 402.2693 401.2620
739 405.2099 404.2026
740 405.2106 404.2033
741 405.2109 404.2036
742 405.2109 404.2036
743 405.2111 404.2038
744 407.2241 406.2168
745 407.2250 406.2177
746 407.2259 406.2186
747 407.2262 406.2189
748 407.2263 406.2190
749 407.2265 406.2192
750 407.2270 406.2197
751 407.2271 406.2198
752 407.2273 406.2200
753 407.2275 406.2202
754 407.2276 406.2203
755 409.2053 408.1980
756 409.2397 408.2324
757 409.2409 408.2336
758 409.2419 408.2346
759 409.2421 408.2348
760 409.2422 408.2349
761 409.2424 408.2351
762 409.2427 408.2354
763 409.2429 408.2356
764 409.2433 408.2360
765 409.2434 408.2361
766 409.2435 408.2362
767 410.2449 409.2376
768 410.2450 409.2377
769 411.2195 410.2122
770 412.2600 411.2527
771 412.2610 411.2537
772 413.2717 412.2644
773 413.2718 412.2645
774 413.2718 412.2645
775 413.2722 412.2649
776 413.2725 412.2652
777 413.2733 412.2660
778 414.2353 413.2280
779 414.2353 413.2280
780 414.2355 413.2282
781 415.2139 414.2066
782 419.1100 418.1027
783 421.2037 420.1964
784 421.2056 420.1983
785 421.2057 420.1984
786 421.2057 420.1984
787 421.2059 420.1986
788 421.2061 420.1988
789 421.2063 420.1990
790 421.2065 420.1992
791 421.2069 420.1996
792 421.2071 420.1998
793 421.2073 420.2000
794 421.2074 420.2001
795 421.2078 420.2005
796 421.2080 420.2007
797 423.1990 422.1917
798 423.2005 422.1932
799 423.2008 422.1935
800 423.2010 422.1937
801 423.2012 422.1939
802 423.2192 422.2119
803 423.2212 422.2139
804 423.2212 422.2139
805 423.2213 422.2140
806 423.2214 422.2141
807 423.2215 422.2142
808 423.2218 422.2145
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