MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
609 379.1956 378.1883
610 379.1959 378.1886
611 379.1960 378.1887
612 379.1965 378.1892
613 379.2303 378.2230
614 379.2660 378.2587
615 381.1930 380.1857
616 381.1932 380.1859
617 381.1936 380.1863
618 381.1944 380.1871
619 381.2094 380.2021
620 381.2107 380.2034
621 381.2108 380.2035
622 381.2114 380.2041
623 381.2119 380.2046
624 381.2120 380.2047
625 381.2121 380.2048
626 381.2126 380.2053
627 381.2130 380.2057
628 381.2131 380.2058
629 381.2149 380.2076
630 381.2444 380.2371
631 383.1879 382.1806
632 383.1900 382.1827
633 383.1902 382.1829
634 383.1909 382.1836
635 383.1925 382.1852
636 383.2076 382.2003
637 383.2076 382.2003
638 383.2076 382.2003
639 383.2076 382.2003
640 383.2083 382.2010
641 383.2085 382.2012
642 383.2090 382.2017
643 383.2091 382.2018
644 383.2095 382.2022
645 383.2099 382.2026
646 383.2111 382.2038
647 383.2256 382.2183
648 383.2270 382.2197
649 383.2273 382.2200
650 383.2278 382.2205
651 383.2279 382.2206
652 383.2280 382.2207
653 383.2283 382.2210
654 383.2285 382.2212
655 383.2286 382.2213
656 383.2296 382.2223
657 383.2298 382.2225
658 383.2303 382.2230
659 385.2015 384.1942
660 385.2045 384.1972
661 385.2046 384.1973
662 385.2204 384.2131
663 387.2378 386.2305
664 387.2392 386.2319
665 387.2587 386.2514
666 389.2141 388.2068
667 391.2301 390.2228
668 391.2315 390.2242
669 391.2318 390.2245
670 391.2878 390.2805
671 391.2879 390.2806
672 391.2879 390.2806
673 391.2880 390.2807
674 391.2880 390.2807
675 391.2886 390.2813
676 391.2889 390.2816
677 391.2892 390.2819
678 393.2107 392.2034
679 393.2456 392.2383
680 393.2469 392.2396
681 393.2470 392.2397
682 393.2472 392.2399
683 393.2477 392.2404
684 393.2479 392.2406
685 393.2992 392.2919
686 393.3008 392.2935
687 393.3010 392.2937
688 393.3010 392.2937
689 393.3012 392.2939
690 393.3014 392.2941
691 393.3014 392.2941
692 393.3017 392.2944
693 393.3019 392.2946
694 393.3021 392.2948
695 393.3024 392.2951
696 393.3027 392.2954
697 393.3028 392.2955
698 393.3028 392.2955
699 393.3029 392.2956
700 393.3029 392.2956
701 393.3030 392.2957
702 395.1910 394.1837
703 395.1913 394.1840
704 395.1920 394.1847
705 395.2264 394.2191
706 395.2266 394.2193
707 395.2266 394.2193
708 395.2268 394.2195
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