MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
509 365.1968 364.1895
510 365.1970 364.1897
511 365.1972 364.1899
512 365.1978 364.1905
513 365.1981 364.1908
514 365.1982 364.1909
515 365.1983 364.1910
516 365.1988 364.1915
517 365.1988 364.1915
518 365.1989 364.1916
519 365.1992 364.1919
520 365.2143 364.2070
521 365.2155 364.2082
522 365.2162 364.2089
523 365.2167 364.2094
524 365.2176 364.2103
525 365.2177 364.2104
526 365.2183 364.2110
527 365.2185 364.2112
528 365.2188 364.2115
529 367.1928 366.1855
530 367.1935 366.1862
531 367.1935 366.1862
532 367.1939 366.1866
533 367.1942 366.1869
534 367.1956 366.1883
535 367.2126 366.2053
536 367.2126 366.2053
537 367.2127 366.2054
538 367.2127 366.2054
539 367.2135 366.2062
540 367.2144 366.2071
541 367.2145 366.2072
542 367.2146 366.2073
543 367.2321 366.2248
544 367.2330 366.2257
545 367.2332 366.2259
546 367.2332 366.2259
547 367.2332 366.2259
548 367.2334 366.2261
549 367.2344 366.2271
550 367.2354 366.2281
551 369.2105 368.2032
552 369.2106 368.2033
553 369.2272 368.2199
554 369.2282 368.2209
555 369.2282 368.2209
556 369.2283 368.2210
557 369.2284 368.2211
558 369.2285 368.2212
559 369.2294 368.2221
560 369.2295 368.2222
561 369.2302 368.2229
562 369.2306 368.2233
563 369.2480 368.2407
564 369.2490 368.2417
565 369.2490 368.2417
566 370.2308 369.2235
567 370.2317 369.2244
568 370.2317 369.2244
569 370.2318 369.2245
570 370.2520 369.2447
571 371.1908 370.1835
572 371.1910 370.1837
573 371.3166 370.3093
574 371.3173 370.3100
575 371.3173 370.3100
576 371.3175 370.3102
577 371.3177 370.3104
578 371.3178 370.3105
579 371.3183 370.3110
580 371.3186 370.3113
581 371.3188 370.3115
582 371.3190 370.3117
583 371.3191 370.3118
584 371.3192 370.3119
585 371.3195 370.3122
586 371.3196 370.3123
587 371.3197 370.3124
588 371.3198 370.3125
589 371.3201 370.3128
590 371.3201 370.3128
591 371.3202 370.3129
592 371.3203 370.3130
593 371.3203 370.3130
594 371.3203 370.3130
595 371.3204 370.3131
596 371.3205 370.3132
597 371.3206 370.3133
598 371.3208 370.3135
599 371.3208 370.3135
600 373.2791 372.2718
601 374.1672 373.1599
602 374.2040 373.1967
603 377.2317 376.2244
604 379.1947 378.1874
605 379.1951 378.1878
606 379.1953 378.1880
607 379.1953 378.1880
608 379.1955 378.1882
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