MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
409 349.2021 348.1948
410 349.2024 348.1951
411 349.2026 348.1953
412 349.2026 348.1953
413 349.2027 348.1954
414 349.2028 348.1955
415 349.2029 348.1956
416 349.2031 348.1958
417 349.2212 348.2139
418 349.2221 348.2148
419 349.2223 348.2150
420 349.2223 348.2150
421 349.2224 348.2151
422 349.2224 348.2151
423 349.2224 348.2151
424 349.2225 348.2152
425 349.2225 348.2152
426 351.1983 350.1910
427 351.1995 350.1922
428 351.1996 350.1923
429 351.1996 350.1923
430 351.1996 350.1923
431 351.1998 350.1925
432 351.2001 350.1928
433 351.2160 350.2087
434 351.2173 350.2100
435 351.2174 350.2101
436 351.2175 350.2102
437 351.2176 350.2103
438 351.2178 350.2105
439 351.2178 350.2105
440 351.2180 350.2107
441 351.2182 350.2109
442 351.2183 350.2110
443 351.2183 350.2110
444 351.2186 350.2113
445 351.2379 350.2306
446 351.2379 350.2306
447 351.2380 350.2307
448 351.2380 350.2307
449 351.2380 350.2307
450 351.2380 350.2307
451 351.2381 350.2308
452 352.2013 351.1940
453 352.2025 351.1952
454 352.2026 351.1953
455 352.2028 351.1955
456 353.0951 352.0878
457 353.1785 352.1712
458 353.1786 352.1713
459 353.1789 352.1716
460 353.1790 352.1717
461 353.1797 352.1724
462 353.1800 352.1727
463 353.1804 352.1731
464 353.1805 352.1732
465 353.1810 352.1737
466 353.2523 352.2450
467 353.2537 352.2464
468 353.2538 352.2465
469 353.2539 352.2466
470 353.2539 352.2466
471 353.2544 352.2471
472 355.1931 354.1858
473 355.1942 354.1869
474 355.1945 354.1872
475 355.1945 354.1872
476 355.1948 354.1875
477 355.1948 354.1875
478 355.1957 354.1884
479 355.1957 354.1884
480 355.1964 354.1891
481 355.1967 354.1894
482 356.2709 355.2636
483 356.2829 355.2756
484 356.2832 355.2759
485 356.2835 355.2762
486 357.2845 356.2772
487 361.0079 360.0006
488 361.1837 360.1764
489 361.1839 360.1766
490 363.1810 362.1737
491 363.1811 362.1738
492 363.1813 362.1740
493 363.1815 362.1742
494 363.1820 362.1747
495 363.1822 362.1749
496 363.1829 362.1756
497 363.1831 362.1758
498 363.2023 362.1950
499 363.2029 362.1956
500 365.1791 364.1718
501 365.1797 364.1724
502 365.1799 364.1726
503 365.1808 364.1735
504 365.1812 364.1739
505 365.1820 364.1747
506 365.1957 364.1884
507 365.1967 364.1894
508 365.1968 364.1895
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