MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
309 335.2232 334.2159
310 335.2234 334.2161
311 335.2234 334.2161
312 335.2236 334.2163
313 335.2402 334.2329
314 335.2429 334.2356
315 335.2430 334.2357
316 335.2430 334.2357
317 335.2431 334.2358
318 335.2436 334.2363
319 336.2257 335.2184
320 337.1827 336.1754
321 337.1835 336.1762
322 337.1836 336.1763
323 337.1838 336.1765
324 337.1838 336.1765
325 337.1840 336.1767
326 337.1842 336.1769
327 337.1843 336.1770
328 337.1846 336.1773
329 337.1855 336.1782
330 337.1863 336.1790
331 337.2004 336.1931
332 337.2013 336.1940
333 337.2014 336.1941
334 337.2015 336.1942
335 337.2016 336.1943
336 337.2019 336.1946
337 337.2025 336.1952
338 337.2032 336.1959
339 337.2382 336.2309
340 337.2569 336.2496
341 337.2574 336.2501
342 337.2574 336.2501
343 337.2576 336.2503
344 338.1671 337.1598
345 338.1671 337.1598
346 338.1676 337.1603
347 338.2405 337.2332
348 338.2412 337.2339
349 338.2415 337.2342
350 339.1613 338.1540
351 339.1625 338.1552
352 339.1635 338.1562
353 339.1640 338.1567
354 339.1658 338.1585
355 339.1982 338.1909
356 339.2006 338.1933
357 339.2010 338.1937
358 339.2739 338.2666
359 341.1478 340.1405
360 341.1488 340.1415
361 341.1785 340.1712
362 341.1792 340.1719
363 341.1795 340.1722
364 341.1796 340.1723
365 341.1959 340.1886
366 341.1963 340.1890
367 341.1964 340.1891
368 341.1969 340.1896
369 341.1971 340.1898
370 341.1973 340.1900
371 341.1975 340.1902
372 341.1977 340.1904
373 341.2171 340.2098
374 341.2171 340.2098
375 341.2172 340.2099
376 341.2889 340.2816
377 342.1903 341.1830
378 343.1931 342.1858
379 343.1983 342.1910
380 343.2319 342.2246
381 343.2973 342.2900
382 345.0489 344.0416
383 345.1452 344.1379
384 346.2603 345.2530
385 347.1847 346.1774
386 347.1858 346.1785
387 347.1859 346.1786
388 347.1859 346.1786
389 347.1860 346.1787
390 347.1862 346.1789
391 347.1864 346.1791
392 347.1867 346.1794
393 347.1867 346.1794
394 347.1868 346.1795
395 347.1870 346.1797
396 347.1871 346.1798
397 347.1875 346.1802
398 347.1889 346.1816
399 347.2055 346.1982
400 347.2059 346.1986
401 347.2060 346.1987
402 349.1820 348.1747
403 349.2007 348.1934
404 349.2014 348.1941
405 349.2016 348.1943
406 349.2018 348.1945
407 349.2020 348.1947
408 349.2021 348.1948
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