MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1258 409.7250 817.4354 817.4334 2.51 0 19 0.13 +1Score > 22 indicates identity VDPFAAAK
3580 685.6888 2054.0446 2054.0168 13.5 0 18 0.11 +1Score > 21 indicates identity ETNPGTDVTVSSVESVLAHL
2115 1105.5758 1104.5685 1104.5676 0.84 1 18 0.082 +1Score > 23 indicates identity
Score > 19 indicates homology		 RDGPASFSLR
1313 845.4374 844.4301 844.4402 -12.0 1 16 0.19 +1Score > 22 indicates identity NARENIK + Deamidated (NQ)
2429 590.3076 1178.6006 1178.5972 2.97 0 16 0.084 +1Score > 23 indicates identity
Score > 18 indicates homology		 YEQHTFILK + Deamidated (NQ)
974 574.3575 573.3502 573.3598 -16.8 1 16 0.3 +1Score > 24 indicates identity
Score > 23 indicates homology		 GRITK
1595 478.2521 954.4896 954.4811 8.99 1 16 0.13 +1Score > 20 indicates identity FKFEDAAK
1060 673.4258 672.4185 672.4170 2.26 0 16 0.17 +1Score > 20 indicates identity KPSITK
1123 366.2090 730.4034 730.3974 8.34 0 15 0.34 +1Score > 23 indicates identity LGQTVGR + Deamidated (NQ)
949 515.3311 514.3238 514.3227 2.14 0 15 0.31 +1Score > 22 indicates identity GALVR
1570 474.2408 946.4670 946.4832 -17.1 0 15 0.13 +1Score > 23 indicates identity
Score > 19 indicates homology		 AGSIQTQSR
1058 337.2162 672.4178 672.4170 1.25 0 15 0.2 +1Score > 20 indicates identity ALTAAVK
1127 735.3327 734.3254 734.3381 -17.3 0 15 0.16 +1Score > 19 indicates identity QLDGMR + Oxidation (M)
2566 647.3563 1292.6980 1292.6837 11.1 0 14 0.22 +1Score > 20 indicates identity HADANVQLVAQK
3066 835.4396 1668.8646 1668.8457 11.3 0 14 0.059 +1Score > 21 indicates identity
Score > 15 indicates homology		 ETGELEELLEPILAN
2656 462.5398 1384.5976 1384.5929 3.41 1 14 0.13 +1Score > 18 indicates identity YNNENGSMPSKK + Deamidated (NQ); Oxidation (M)
1143 372.2070 742.3994 742.4086 -12.3 1 14 0.12 +1Score > 18 indicates identity AGRTGPGK
1782 508.7582 1015.5018 1015.4934 8.31 0 14 0.26 +1Score > 21 indicates identity GLNNGENVAK + Deamidated (NQ)
2171 560.3149 1118.6152 1118.6118 3.11 1 14 0.33 +1Score > 22 indicates identity IMVIGGKNSGK + Oxidation (M)
1934 1058.4935 1057.4862 1057.4862 -0.012 1 14 0.16 +1Score > 18 indicates identity MYDTTQKR + Oxidation (M)
1314 423.2226 844.4306 844.4403 -11.4 1 14 0.17 +1Score > 22 indicates identity
Score > 18 indicates homology		 EGGREGLK
1259 410.2165 818.4184 818.4021 19.9 0 13 0.4 +1Score > 23 indicates identity
Score > 22 indicates homology		 VDSLEEK
2757 734.8581 1467.7016 1467.6994 1.55 1 13 0.12 +1Score > 22 indicates identity
Score > 17 indicates homology		 AIPNNSFRDSFAK + 2 Deamidated (NQ)
1801 511.7688 1021.5230 1021.5226 0.42 0 13 0.13 +1Score > 22 indicates identity
Score > 17 indicates homology		 MAGATSSIIR + Oxidation (M)
1169 380.7144 759.4142 759.4239 -12.7 1 13 0.27 +1Score > 24 indicates identity
Score > 19 indicates homology		 RISQEK
1059 337.2162 672.4178 672.4170 1.25 1 13 0.34 +1Score > 20 indicates identity VAKDLK
1027 644.3370 643.3297 643.3289 1.23 0 12 0.18 +1Score > 18 indicates identity LGSPDR
2375 583.7786 1165.5426 1165.5397 2.53 0 12 0.16 +1Score > 22 indicates identity
Score > 17 indicates homology		 NINLSEMSSR + Oxidation (M)
3014 544.6074 1630.8004 1630.7910 5.73 1 12 0.084 +1Score > 23 indicates identity
Score > 14 indicates homology		 NSTNASVAGNISNPKR + 2 Deamidated (NQ)
2961 531.2783 1590.8131 1590.8253 -7.69 1 12 0.59 +1Score > 22 indicates identity LDPTIYESIQREK
1006 618.3292 617.3219 617.3133 14.0 1 12 0.75 +1Score > 23 indicates identity KNGNGK + Deamidated (NQ)
1547 470.7638 939.5130 939.5066 6.89 0 12 0.26 +1Score > 19 indicates identity FLGFDTIK
2427 590.2876 1178.5606 1178.5390 18.4 0 12 0.61 +1Score > 22 indicates identity AAFTPEMQER
3470 661.3391 1980.9955 1980.9792 8.20 1 12 0.16 +1Score > 22 indicates identity
Score > 16 indicates homology		 NLDTFLNQLRDFLNEK + 2 Deamidated (NQ)
1061 338.1770 674.3394 674.3421 -4.00 0 12 0.38 +1Score > 22 indicates identity
Score > 20 indicates homology		 TCLGPK
3336 624.6148 1870.8226 1870.8405 -9.57 1 12 0.3 +1Score > 19 indicates identity NENKVNNATNATHNNSK + 2 Deamidated (NQ)
1208 397.2277 792.4408 792.4381 3.42 0 12 0.15 +1Score > 21 indicates identity
Score > 16 indicates homology		 LSFEIGK
2180 1122.5090 1121.5017 1121.5209 -17.1 0 12 0.13 +1Score > 21 indicates identity
Score > 15 indicates homology		 AGGEGMCIAIK + Oxidation (M)
1030 322.6725 643.3304 643.3289 2.35 0 12 0.22 +1Score > 18 indicates identity LGSPDR
2676 705.8550 1409.6954 1409.7006 -3.68 1 12 0.49 +1Score > 21 indicates identity
Score > 21 indicates homology		 NTNISIMRLGMK + Deamidated (NQ); 2 Oxidation (M)
1548 470.7659 939.5172 939.5066 11.4 0 12 0.28 +1Score > 19 indicates identity FLGFDTIK
1675 984.4675 983.4602 983.4784 -18.5 0 12 0.38 +1Score > 20 indicates identity NPSQRPQR + 2 Deamidated (NQ)
1468 914.4390 913.4317 913.4253 6.99 1 11 0.29 +1Score > 19 indicates identity DKHSENGK
2731 724.3858 1446.7570 1446.7579 -0.60 0 11 0.73 +1Score > 22 indicates identity LGATIDVEHSHLR
2882 773.9079 1545.8012 1545.8012 0.055 1 11 0.73 +1Score > 22 indicates identity VASHKNSHNPSLVR + Deamidated (NQ)
1531 468.2395 934.4644 934.4509 14.6 0 11 0.65 +1Score > 22 indicates identity NPTNFTGGK
1091 351.1833 700.3520 700.3504 2.39 0 11 0.3 +1Score > 18 indicates identity INPSNR + Deamidated (NQ)
2732 724.3860 1446.7574 1446.7579 -0.33 0 11 0.76 +1Score > 22 indicates identity LGATIDVEHSHLR
2589 659.3477 1316.6808 1316.6646 12.4 1 11 0.85 +1Score > 23 indicates identity LKDGPGMDILNK + Deamidated (NQ); Oxidation (M)
2868 769.8613 1537.7080 1537.7195 -7.42 1 11 0.66 +1Score > 21 indicates identity KPTNQDYRMQQK + 2 Deamidated (NQ)
1011 313.1876 624.3606 624.3707 -16.1 1 11 0.33 +1Score > 18 indicates identity VKHNK
2295 575.2871 1148.5596 1148.5713 -10.1 0 11 0.36 +1Score > 22 indicates identity
Score > 19 indicates homology		 NLPSLEANYK + Deamidated (NQ)
2522 626.8150 1251.6154 1251.6064 7.27 1 10 1 +1Score > 24 indicates identity
Score > 23 indicates homology		 MSLKQMQSQR + Oxidation (M)
2733 724.3868 1446.7590 1446.7579 0.78 0 10 0.8 +1Score > 22 indicates identity
Score > 22 indicates homology		 LGATIDVEHSHLR
1231 400.7176 799.4206 799.4301 -11.8 0 10 0.37 +1Score > 18 indicates identity LLGGGGGGGR
2274 573.2852 1144.5558 1144.5724 -14.4 0 10 0.42 +1Score > 22 indicates identity
Score > 19 indicates homology		 ENGLLNEISR + Deamidated (NQ)
1209 397.2278 792.4410 792.4381 3.67 0 10 0.71 +1Score > 21 indicates identity LSFEIGK
3192 880.8591 1759.7036 1759.7384 -19.7 0 10 0.13 +1Score > 14 indicates identity TGNEDVGNNNPSNSIPK + 4 Deamidated (NQ)
1015 630.3309 629.3236 629.3133 16.4 0 10 0.23 +1Score > 16 indicates identity TAQPGR + Deamidated (NQ)
1005 309.6665 617.3184 617.3133 8.40 1 10 0.43 +1Score > 23 indicates identity
Score > 19 indicates homology		 KNGNGK + Deamidated (NQ)
1872 1042.5184 1041.5111 1041.5091 1.96 0 10 0.56 +1Score > 20 indicates identity
Score > 20 indicates homology		 SEQHSVAVGK + Deamidated (NQ)
2949 529.5916 1585.7530 1585.7372 9.95 1 10 0.78 +1Score > 21 indicates identity SREAFSYEQAQLR + 2 Deamidated (NQ)
1542 939.4665 938.4592 938.4709 -12.4 0 10 0.24 +1Score > 20 indicates identity
Score > 16 indicates homology		 NVTDYLSK
1133 370.7012 739.3878 739.3864 1.90 0 10 0.38 +1Score > 18 indicates identity HIVEDK
3151 580.2651 1737.7735 1737.7614 6.93 0 10 0.53 +1Score > 20 indicates identity MESQQLSQNSPTLQK + 4 Deamidated (NQ); Oxidation (M)
2880 773.9030 1545.7914 1545.8012 -6.28 1 10 0.47 +1Score > 22 indicates identity
Score > 19 indicates homology		 VASHKNSHNPSLVR + Deamidated (NQ)
3410 972.4502 1942.8858 1942.8652 10.6 0 10 0.34 +1Score > 22 indicates identity
Score > 18 indicates homology		 TGCGEMDLFEILTAGSDK
1177 767.4070 766.3997 766.3973 3.13 0 10 0.38 +1Score > 20 indicates identity
Score > 18 indicates homology		 KPEQHK + Deamidated (NQ)
1040 329.1822 656.3498 656.3605 -16.3 0 10 0.26 +1Score > 16 indicates identity LNAPSR
1889 1048.5188 1047.5115 1047.5322 -19.8 1 10 0.21 +1Score > 22 indicates identity
Score > 15 indicates homology		 RTGSHTHPR
2343 1160.5624 1159.5551 1159.5770 -18.8 0 10 0.23 +1Score > 21 indicates identity
Score > 16 indicates homology		 MVPTMFPPPK + Oxidation (M)
1652 488.2690 974.5234 974.5080 15.9 1 10 0.9 +1Score > 22 indicates identity MIQNASRR
1065 340.6851 679.3556 679.3541 2.31 0 10 0.17 +1Score > 22 indicates identity
Score > 14 indicates homology		 YAPTTK
2630 451.8979 1352.6719 1352.6725 -0.43 0 9 1 +1Score > 22 indicates identity
Score > 22 indicates homology		 VANGNNVVQFYK + Deamidated (NQ)
1090 701.3582 700.3509 700.3504 0.79 0 9 0.36 +1Score > 17 indicates identity INPSNR + Deamidated (NQ)
1240 403.6989 805.3832 805.3752 10.00 0 9 0.71 +1Score > 21 indicates identity
Score > 21 indicates homology		 MSNANLR + Deamidated (NQ)
1932 528.7712 1055.5278 1055.5360 -7.68 1 9 0.44 +1Score > 20 indicates identity
Score > 18 indicates homology		 KESQPPNTR
3878 1097.0416 2192.0686 2192.0566 5.51 1 9 0.26 +1Score > 22 indicates identity
Score > 16 indicates homology		 DNVDVNGRLGTNMIVQGVMK + Deamidated (NQ); 2 Oxidation (M)
1311 844.4421 843.4348 843.4450 -12.1 0 9 0.55 +1Score > 19 indicates identity QNLINNK + Deamidated (NQ)
953 532.2892 531.2819 531.2805 2.65 0 9 0.31 +1Score > 22 indicates identity
Score > 17 indicates homology		 AWAGK
2493 608.3565 1214.6984 1214.6758 18.7 0 9 0.47 +1Score > 19 indicates identity LDILQAIVNSK + 2 Deamidated (NQ)
1387 879.4587 878.4514 878.4538 -2.70 0 9 0.15 +1Score > 22 indicates identity
Score > 14 indicates homology		 YFPDLPK
3827 718.0206 2151.0400 2151.0452 -2.44 1 9 1 +1Score > 22 indicates identity
Score > 22 indicates homology		 RSPTAECTALGAAIAANMAFK + Deamidated (NQ)
1122 366.1828 730.3510 730.3609 -13.6 0 9 0.51 +1Score > 19 indicates identity NGIGQNK + Deamidated (NQ)
1541 470.2364 938.4582 938.4709 -13.5 0 9 0.37 +1Score > 20 indicates identity
Score > 17 indicates homology		 NVTDYLSK
2859 767.3741 1532.7336 1532.7293 2.84 1 9 0.98 +1Score > 21 indicates identity SYKANQPSPGEMPK
2537 633.8273 1265.6400 1265.6292 8.57 0 9 0.54 -1Score > 22 indicates identity
Score > 19 indicates homology		 FLQGVNSFEPK + Deamidated (NQ)
4164 830.0792 2487.2158 2487.1675 19.4 1 9 0.99 +1Score > 21 indicates identity FVNMRTGVAGFNDSLMNHLYGK + Deamidated (NQ); Oxidation (M)
1931 1056.5339 1055.5266 1055.5400 -12.7 1 9 0.46 +1Score > 20 indicates identity
Score > 18 indicates homology		 RSGTFFNVK + Deamidated (NQ)
2097 551.2821 1100.5496 1100.5462 3.16 0 9 0.31 +1Score > 21 indicates identity
Score > 16 indicates homology		 IQAEGGAADIR + Deamidated (NQ)
1004 616.3683 615.3610 615.3592 3.03 0 9 1 +1Score > 25 indicates identity
Score > 21 indicates homology		 AVIEGK
2555 640.8152 1279.6158 1279.6329 -13.4 1 9 1 +1Score > 23 indicates identity
Score > 21 indicates homology		 KTSQLMEEIGK + Deamidated (NQ); Oxidation (M)
1021 319.1875 636.3604 636.3707 -16.2 0 9 0.19 +1Score > 20 indicates identity
Score > 14 indicates homology		 QHKPK
2902 781.9047 1561.7948 1561.8239 -18.6 0 9 0.17 +1Score > 23 indicates identity
Score > 13 indicates homology		 ATLSTLFQELNVPK + 2 Deamidated (NQ)
1434 450.2711 898.5276 898.5283 -0.71 1 9 0.53 +1Score > 18 indicates identity ARLMRPR
1882 523.7792 1045.5438 1045.5444 -0.52 0 8 0.77 +1Score > 23 indicates identity
Score > 20 indicates homology		 LASWVIDNK + Deamidated (NQ)
4503 790.3970 3157.5589 3157.6118 -16.7 0 8 0.91 +1Score > 21 indicates identity QDLLPPDINATNGKPLSMAPFLNLFGTTR + Deamidated (NQ); Oxidation (M)
1022 637.3685 636.3612 636.3707 -14.9 0 8 0.36 +1Score > 16 indicates identity QHKPK
3102 849.3840 1696.7534 1696.7726 -11.3 0 8 0.81 +1Score > 20 indicates identity SMEQNYLRPEIGSR + 2 Deamidated (NQ); Oxidation (M)
2576 651.8713 1301.7280 1301.7455 -13.4 1 8 0.34 +1Score > 20 indicates identity
Score > 16 indicates homology		 SLDLYLAPRVR
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