MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 3 |
| MS data file | : | PRT1245_Other_P1_B11.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:21:58 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,722 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO1_YEAST | 484 | Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | G3P1_YEAST | 267 | Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH1 PE=1 SV=3 | |
| 2 | G3P2_YEAST | 201 | Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH2 PE=1 SV=3 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGK_YEAST | 258 | Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 3.1 | PGK_YEAST |
258 | 44768 | 9 (8) | 8 (8) | 0.77 | |
| Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2 | |||||||
9 peptide matches (9 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1401 |
443.7679 | 885.5212 | 885.5171 | 4.66 | 0 | 54 | 2.7e-005 | 1Score > 20 indicates identity |
U | K.ALLDEVVK.S | |
| 1493 |
463.3112 | 924.6078 | 924.6008 | 7.64 | 0 | 45 | 3.3e-005 | 1Score > 13 indicates identity |
U | R.IVAALPTIK.Y | |
| 1831 |
515.7968 | 1029.5790 | 1029.5706 | 8.19 | 0 | 47 | 0.00012 | 1Score > 21 indicates identity Score > 20 indicates homology |
U | K.LSVQDLDLK.D | |
| 2105 |
552.2973 | 1102.5800 | 1102.5659 | 12.8 | 0 | 51 | 7.5e-005 | 1Score > 22 indicates identity |
U | R.VDFNVPLDGK.K | |
| 2457 |
595.3367 | 1188.6588 | 1188.6390 | 16.7 | 0 | 51 | 3.2e-005 | 1Score > 21 indicates identity Score > 19 indicates homology |
U | K.ELPGVAFLSEK.K | |
| 2703 |
720.4252 | 1438.8358 | 1438.8143 | 14.9 | 0 | 59 | 4.9e-006 | 1Score > 18 indicates identity |
U | K.ASAPGSVILLENLR.Y | |
D:\Xcalibur\Data\Yan\PRT1245 redo\PRT1245_Other_P1_B11.rawScore > 20 indicates identity |
|||||||||||
| 3182 |
585.6511 | 1753.9315 | 1753.9210 | 5.96 | 0 | 57 | 1.2e-005 | 1Score > 20 indicates identity |
U | K.ISHVSTGGGASLELLEGK.E | |
| No other peptide matches in query | |||||||||||
| 4094 |
1196.6396 | 2391.2646 | 2391.2210 | 18.3 | 0 | 14 | 0.2 | 1Score > 19 indicates identity |
U | K.GVEVVLPVDFIIADAFSADANTK.T + Deamidated (NQ) | |
| 4095 |
798.0956 | 2391.2650 | 2391.2210 | 18.4 | 0 | 27 | 0.0086 | 1Score > 19 indicates identity |
U | K.GVEVVLPVDFIIADAFSADANTK.T + Deamidated (NQ) | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A0A8H6ZX18_PLEOS | 111 | Peroxidase OS=Pleurotus ostreatus OX=5322 GN=VPL1_1 PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9FD70_ENTFL | 110 | Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EXG1_YEAST | 94 | Glucan 1,3-beta-glucosidase I/II OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EXG1 PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PMG1_YEAST | 92 | Phosphoglycerate mutase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GPM1 PE=1 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SDO1L_YEAST | 76 | Restriction of telomere capping protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTC3 PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SCW4_YEAST | 60 | Probable family 17 glucosidase SCW4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCW4 PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HSP12_YEAST | 57 | 12 kDa heat shock protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP12 PE=1 SV=1 |
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