MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 3 |
| MS data file | : | PRT1245_Other_P1_B11.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:21:58 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,722 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO1_YEAST | 484 | Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | G3P1_YEAST | 267 | Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH1 PE=1 SV=3 | |
| 2 | G3P2_YEAST | 201 | Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH2 PE=1 SV=3 | |
9 peptide matches (7 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | 1 | 2 | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1396 |
442.2687 | 882.5228 | 882.5175 | 6.11 | 0 | 31 | 0.0015 | 1Score > 15 indicates identity |
U | |
|
K.VLPELQGK.L | |
| 2129 |
370.2184 | 1107.6334 | 1107.6288 | 4.11 | 0 | 39 | 0.00046 | 1Score > 19 indicates identity |
U | |
R.VVDLVEHVAK.A | ||
| 2288 |
1 |
574.8406 | 1147.6666 | 1147.6489 | 15.5 | 0 | 45 | 0.00011 | 1Score > 18 indicates identity |
U | |
R.VVDLIEYVAK.A | |
| 2471 |
599.3259 | 1196.6372 | 1196.6190 | 15.3 | 0 | 49 | 7.7e-005 | 1Score > 21 indicates identity Score > 20 indicates homology |
U | |
R.DPANLPWGSLK.I | ||
| 2521 |
625.8401 | 1249.6656 | 1249.6554 | 8.17 | 0 | 31 | 0.0092 | 1Score > 23 indicates identity |
U | |
K.IDVAVDSTGVFK.E | ||
| 2759 |
1 |
735.9336 | 1469.8526 | 1469.8342 | 12.6 | 0 | 77 | 4.8e-008 | 1Score > 16 indicates identity |
U | |
|
R.VPTVDVSVVDLTVK.L |
| 2760 |
490.9587 | 1469.8543 | 1469.8342 | 13.7 | 0 | 54 | 7.5e-006 | 1Score > 15 indicates identity |
U | |
|
R.VPTVDVSVVDLTVK.L |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGK_YEAST | 258 | Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A0A8H6ZX18_PLEOS | 111 | Peroxidase OS=Pleurotus ostreatus OX=5322 GN=VPL1_1 PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9FD70_ENTFL | 110 | Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EXG1_YEAST | 94 | Glucan 1,3-beta-glucosidase I/II OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EXG1 PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PMG1_YEAST | 92 | Phosphoglycerate mutase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GPM1 PE=1 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SDO1L_YEAST | 76 | Restriction of telomere capping protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTC3 PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SCW4_YEAST | 60 | Probable family 17 glucosidase SCW4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCW4 PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HSP12_YEAST | 57 | 12 kDa heat shock protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP12 PE=1 SV=1 |
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