MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (26 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 ENO1_YEAST 484 46844 16 (15) 12 (12) 1.59 Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3
2.1 G3P1_YEAST 267 35842 8 (8) 5 (5) 0.70 Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH1 PE=1 SV=3
2.2 G3P2_YEAST 201 35938 5 (5) 3 (3) 0.42 Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH2 PE=1 SV=3
3 PGK_YEAST 258 44768 9 (8) 8 (8) 0.77 Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2
4 A0A8H6ZX18_PLEOS 111 37983 3 (3) 2 (2) 0.18 Peroxidase OS=Pleurotus ostreatus OX=5322 GN=VPL1_1 PE=3 SV=1
5 Q9FD70_ENTFL 110 86614 2 (1) 1 (1) 0.04 Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1
6 EXG1_YEAST 94 51735 6 (5) 5 (4) 0.36 Glucan 1,3-beta-glucosidase I/II OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EXG1 PE=1 SV=1
7 PMG1_YEAST 92 27592 3 (3) 3 (3) 0.41 Phosphoglycerate mutase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GPM1 PE=1 SV=3
8 SDO1L_YEAST 76 12002 3 (3) 2 (2) 1.13 Restriction of telomere capping protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTC3 PE=1 SV=1
9 SCW4_YEAST 60 40434 3 (2) 3 (2) 0.17 Probable family 17 glucosidase SCW4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCW4 PE=1 SV=1
10 HSP12_YEAST 57 11686 2 (2) 2 (2) 0.68 12 kDa heat shock protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP12 PE=1 SV=1
11 PIR1_YEAST 53 34829 1 (1) 1 (1) 0.10 Cell wall mannoprotein PIR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIR1 PE=1 SV=1
12 CYPH_YEAST 40 17494 1 (1) 1 (1) 0.19 Peptidyl-prolyl cis-trans isomerase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CPR1 PE=1 SV=3
13 PDC1_YEAST 40 61685 1 (1) 1 (1) 0.05 Pyruvate decarboxylase isozyme 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PDC1 PE=1 SV=7
14 NACB2_YEAST 39 16724 1 (1) 1 (1) 0.20 Nascent polypeptide-associated complex subunit beta-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BTT1 PE=1 SV=1
15 HSP26_YEAST 39 23865 1 (1) 1 (1) 0.14 Heat shock protein 26 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP26 PE=1 SV=3
16 HPC2_YEAST 36 0 1 (1) 1 (1) 0.05 description
17 ATP7_YEAST 35 0 1 (1) 1 (1) 0.17 description
18 MFAL1_YEAST 35 18630 1 (1) 1 (1) 0.18 Mating factor alpha-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MF(ALPHA)1 PE=1 SV=1
19 KPR2_YEAST 32 35085 3 (2) 1 (1) 0.20 Ribose-phosphate pyrophosphokinase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PRS2 PE=1 SV=1
20 FAB1_YEAST 30 258743 3 (1) 1 (1) 0.01 1-phosphatidylinositol 3-phosphate 5-kinase FAB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FAB1 PE=1 SV=3
21 YN034_YEAST 26 10775 1 (1) 1 (1) 0.32 Uncharacterized protein YNR034W-A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YNR034W-A PE=1 SV=1
22 B211p2_002|NRPSb 23 633771 3 (1) 3 (1) 0.01 OS=Anaeromyces robustus OX=1754192 PE=2 SV=1
23 BMH1_YEAST 22 30187 1 (1) 1 (1) 0.11 Protein BMH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BMH1 PE=1 SV=4
24 RTC4_YEAST 22 46468 2 (1) 1 (1) 0.07 Restriction of telomere capping protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTC4 PE=1 SV=1
25 SERC_YEAST 17 43560 1 (1) 1 (1) 0.08 Phosphoserine aminotransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SER1 PE=1 SV=1

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