MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 3 |
| MS data file | : | PRT1245_Other_P1_B11.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:21:58 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,722 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO1_YEAST | 484 | Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 1.1 | ENO1_YEAST |
484 | 46844 | 16 (15) | 12 (12) | 1.59 | |
| Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3 | |||||||
16 peptide matches (14 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1228 |
400.6964 | 799.3782 | 799.3752 | 3.80 | 0 | 28 | 0.0043 | 1Score > 17 indicates identity |
U | K.YDLDFK.N | |
| 1241 |
404.2236 | 806.4326 | 806.4286 | 4.98 | 0 | 40 | 0.00063 | 1Score > 22 indicates identity Score > 20 indicates homology |
U | K.TFAEALR.I | |
| 1252 |
407.7579 | 813.5012 | 813.4960 | 6.49 | 0 | 44 | 0.00022 | 1Score > 20 indicates identity |
U | K.AADALLLK.V | |
| 2561 |
644.3585 | 1286.7024 | 1286.6870 | 12.0 | 0 | 37 | 0.00036 | 1Score > 21 indicates identity Score > 15 indicates homology |
U | K.NVNDVIAPAFVK.A + Deamidated (NQ) | |
D:\Xcalibur\Data\Yan\PRT1245 redo\PRT1245_Other_P1_B11.rawScore > 21 indicates identity Score > 21 indicates homology |
|||||||||||
| 2563 |
645.3550 | 1288.6954 | 1288.6874 | 6.23 | 0 | 47 | 0.00012 | 1Score > 21 indicates identity Score > 21 indicates homology |
U | K.VNQIGTLSESIK.A + Deamidated (NQ) | |
| 6.23 | 0 | 36 | 0.0016 | 2 | K.VNQIGTLSESIK.A + Deamidated (NQ) | ||||||
| 20.0 | 0 | 5 | 1.8 | 3 | LQVNGITMADVK + Deamidated (NQ) | ||||||
| 2679 |
708.8691 | 1415.7236 | 1415.7144 | 6.54 | 0 | 45 | 0.00032 | 1Score > 23 indicates identity |
U | R.GNPTVEVELTTEK.G | |
| 2801 |
500.2807 | 1497.8203 | 1497.8191 | 0.79 | 1 | 42 | 0.00028 | 1Score > 19 indicates identity |
U | K.NVPLYKHLADLSK.S + Deamidated (NQ) | |
| 2931 |
1 |
526.9459 | 1577.8159 | 1577.7937 | 14.1 | 0 | 42 | 0.0002 | 1Score > 22 indicates identity Score > 18 indicates homology |
U | K.AVDDFLISLDGTANK.S |
| 2933 |
789.9178 | 1577.8210 | 1577.7937 | 17.3 | 0 | 59 | 1.4e-005 | 1Score > 23 indicates identity |
U | K.AVDDFLISLDGTANK.S | |
| 3183 |
585.9963 | 1754.9671 | 1754.9414 | 14.6 | 0 | 71 | 2.5e-007 | 1Score > 18 indicates identity |
U | K.TAGIQIVADDLTVTNPK.R | |
| 3256 |
607.9861 | 1820.9365 | 1820.9156 | 11.5 | 0 | 28 | 0.004 | 1Score > 22 indicates identity Score > 17 indicates homology |
U | R.SGETEDTFIADLVVGLR.T | |
| 3258 |
1 |
911.4817 | 1820.9488 | 1820.9156 | 18.2 | 0 | 75 | 1.2e-007 | 1Score > 22 indicates identity Score > 18 indicates homology |
U | R.SGETEDTFIADLVVGLR.T |
| 3284 |
614.3163 | 1839.9271 | 1839.9149 | 6.62 | 0 | 55 | 2.6e-005 | 1Score > 22 indicates identity |
U | R.SIVPSGASTGVHEALEMR.D | |
| 4533 |
1086.8860 | 3257.6362 | 3257.5939 | 13.0 | 0 | 76 | 1.6e-007 | 1Score > 21 indicates identity |
U | R.YGASAGNVGDEGGVAPNIQTAEEALDLIVDAIK.A + Deamidated (NQ) | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ENO2_YEAST |
366 | 46942 | 1.1 | ||
| Enolase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO2 PE=1 SV=2 | |||||
| ERR1_YEAST |
28 | 47697 | 1.1 | ||
| Enolase-related protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR1 PE=3 SV=1 | |||||
| 2 samesets of ERR1_YEAST | |||||
| ERR3_YEAST |
28 | 47682 | |||
| Enolase-related protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR3 PE=3 SV=2 | |||||
| ERR2_YEAST |
28 | 47697 | |||
| Enolase-related protein 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR2 PE=3 SV=1 | |||||
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | G3P1_YEAST | 267 | Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH1 PE=1 SV=3 | |
| 2 | G3P2_YEAST | 201 | Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH2 PE=1 SV=3 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGK_YEAST | 258 | Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A0A8H6ZX18_PLEOS | 111 | Peroxidase OS=Pleurotus ostreatus OX=5322 GN=VPL1_1 PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9FD70_ENTFL | 110 | Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EXG1_YEAST | 94 | Glucan 1,3-beta-glucosidase I/II OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EXG1 PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PMG1_YEAST | 92 | Phosphoglycerate mutase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GPM1 PE=1 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SDO1L_YEAST | 76 | Restriction of telomere capping protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTC3 PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SCW4_YEAST | 60 | Probable family 17 glucosidase SCW4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCW4 PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HSP12_YEAST | 57 | 12 kDa heat shock protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP12 PE=1 SV=1 |
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