MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
135 321.1701 320.1628
136 321.1706 320.1633
137 321.1707 320.1634
138 321.1707 320.1634
139 321.2061 320.1988
140 321.2061 320.1988
141 321.2065 320.1992
142 321.2428 320.2355
143 321.2431 320.2358
144 321.2431 320.2358
145 321.2619 320.2546
146 322.2453 321.2380
147 323.1679 322.1606
148 323.1681 322.1608
149 323.1685 322.1612
150 323.1687 322.1614
151 323.1688 322.1615
152 323.1856 322.1783
153 323.1859 322.1786
154 323.1859 322.1786
155 323.1861 322.1788
156 323.1864 322.1791
157 323.1869 322.1796
158 323.1869 322.1796
159 323.1872 322.1799
160 323.1880 322.1807
161 323.1885 322.1812
162 323.2041 322.1968
163 323.2059 322.1986
164 323.2062 322.1989
165 325.1821 324.1748
166 325.1833 324.1760
167 325.1837 324.1764
168 325.1838 324.1765
169 325.1838 324.1765
170 325.1841 324.1768
171 325.1847 324.1774
172 325.1852 324.1779
173 325.1865 324.1792
174 325.2016 324.1943
175 325.2021 324.1948
176 325.2025 324.1952
177 325.2218 324.2145
178 325.2219 324.2146
179 325.2939 324.2866
180 326.1478 325.1405
181 327.1331 326.1258
182 327.1983 326.1910
183 327.2008 326.1935
184 327.2012 326.1939
185 327.2014 326.1941
186 327.2017 326.1944
187 327.2018 326.1945
188 327.2020 326.1947
189 327.2176 326.2103
190 327.2180 326.2107
191 327.2182 326.2109
192 327.2187 326.2114
193 327.2365 326.2292
194 329.1776 328.1703
195 329.1936 328.1863
196 329.1939 328.1866
197 329.2166 328.2093
198 331.1907 330.1834
199 331.1907 330.1834
200 331.1909 330.1836
201 331.1909 330.1836
202 331.1909 330.1836
203 331.1915 330.1842
204 331.1915 330.1842
205 331.2105 330.2032
206 331.2111 330.2038
207 331.2116 330.2043
208 333.2056 332.1983
209 333.2067 332.1994
210 333.2068 332.1995
211 333.2069 332.1996
212 333.2071 332.1998
213 333.2073 332.2000
214 333.2073 332.2000
215 333.2075 332.2002
216 333.2076 332.2003
217 333.2077 332.2004
218 333.2077 332.2004
219 333.2248 332.2175
220 333.2270 332.2197
221 333.2273 332.2200
222 333.2274 332.2201
223 333.2277 332.2204
224 335.1865 334.1792
225 335.1870 334.1797
226 335.1875 334.1802
227 335.2212 334.2139
228 335.2223 334.2150
229 335.2224 334.2151
230 335.2225 334.2152
231 335.2225 334.2152
232 335.2229 334.2156
233 335.2230 334.2157
234 335.2232 334.2159
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