MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
35 307.1909 306.1836
36 307.1910 306.1837
37 307.1912 306.1839
38 307.1915 306.1842
39 307.1916 306.1843
40 307.1921 306.1848
41 307.1921 306.1848
42 307.1923 306.1850
43 307.2096 306.2023
44 308.0897 307.0824
45 308.0898 307.0825
46 308.0899 307.0826
47 308.0905 307.0832
48 308.0911 307.0838
49 308.0919 307.0846
50 308.0920 307.0847
51 308.0921 307.0848
52 308.0922 307.0849
53 308.0922 307.0849
54 308.0924 307.0851
55 308.0927 307.0854
56 308.0928 307.0855
57 308.0928 307.0855
58 308.0929 307.0856
59 308.0929 307.0856
60 308.0930 307.0857
61 308.0930 307.0857
62 309.1704 308.1631
63 309.1704 308.1631
64 309.1705 308.1632
65 309.1706 308.1633
66 309.1880 308.1807
67 309.1891 308.1818
68 309.1892 308.1819
69 309.1897 308.1824
70 309.1903 308.1830
71 309.1918 308.1845
72 309.2072 308.1999
73 309.2077 308.2004
74 309.2087 308.2014
75 309.2095 308.2022
76 309.2101 308.2028
77 310.1912 309.1839
78 310.1922 309.1849
79 311.1677 310.1604
80 311.1678 310.1605
81 311.1679 310.1606
82 311.1679 310.1606
83 311.1682 310.1609
84 311.1686 310.1613
85 311.1702 310.1629
86 311.1858 310.1785
87 311.1863 310.1790
88 311.2050 310.1977
89 311.2062 310.1989
90 311.2067 310.1994
91 311.2067 310.1994
92 311.2083 310.2010
93 311.2230 310.2157
94 311.2240 310.2167
95 311.2241 310.2168
96 311.2245 310.2172
97 311.2249 310.2176
98 312.1328 311.1255
99 312.1329 311.1256
100 312.1331 311.1258
101 312.1336 311.1263
102 313.1221 312.1148
103 313.1223 312.1150
104 313.1838 312.1765
105 313.1841 312.1768
106 313.1842 312.1769
107 313.1843 312.1770
108 313.1844 312.1771
109 313.1850 312.1777
110 313.1859 312.1786
111 313.1863 312.1790
112 313.2213 312.2140
113 313.2222 312.2149
114 313.2222 312.2149
115 313.2402 312.2329
116 315.2016 314.1943
117 318.1299 317.1226
118 319.1892 318.1819
119 319.1904 318.1831
120 319.1904 318.1831
121 319.1906 318.1833
122 319.1908 318.1835
123 319.1912 318.1839
124 319.1914 318.1841
125 319.2261 318.2188
126 319.2271 318.2198
127 319.2272 318.2199
128 319.2272 318.2199
129 319.2273 318.2200
130 319.2276 318.2203
131 319.2277 318.2204
132 321.1698 320.1625
133 321.1699 320.1626
134 321.1700 320.1627
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