MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1151 794.3685 793.3612 793.3640 -3.52 0 2 0.96 +1Score > 21 indicates identity
Score > 15 indicates homology		 TTCGDLK
3052 757.9156 1513.8166 1513.8075 6.06 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology		 KPTMIKLQFHNR + 2 Deamidated (NQ)
1205 824.4266 823.4193 823.4188 0.64 0 2 0.68 +1Score > 20 indicates identity
Score > 13 indicates homology		 GALPSHNK + Deamidated (NQ)
2245 549.7664 1097.5182 1097.5076 9.66 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology		 FARTWDMR + Oxidation (M)
2619 592.7537 1183.4928 1183.5027 -8.29 1 2 1.1 +1Score > 15 indicates identity MTKTNNTNDK + 2 Deamidated (NQ); Oxidation (M)
3136 782.3698 1562.7250 1562.7424 -11.1 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology		 LEGRGTETEESINK + Deamidated (NQ)
4011 1096.5345 2191.0544 2191.0433 5.07 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology		 EIPDGEVVQFKTFSTNDHK + Deamidated (NQ)
934 667.3530 666.3457 666.3561 -15.6 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology		 LNHQR
3750 684.6489 2050.9249 2050.9201 2.35 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology		 TEQERGHVASCIDCYVK
827 584.3412 583.3339 583.3442 -17.6 0 2 0.6 +1Score > 13 indicates identity VAAPAR
2530 1165.5513 1164.5440 1164.5332 9.27 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology		 NMTKIDSPDK + Deamidated (NQ); Oxidation (M)
2742 630.3028 1258.5910 1258.6153 -19.3 1 2 0.88 +1Score > 20 indicates identity
Score > 14 indicates homology		 NEQDVLERQK + Deamidated (NQ)
3672 664.6440 1990.9102 1990.9265 -8.22 0 2 0.71 +1Score > 21 indicates identity
Score > 13 indicates homology		 AANLGGVAVSGLEMAQNSQR + 3 Deamidated (NQ); Oxidation (M)
4398 926.4377 2776.2913 2776.3436 -18.8 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology		 TLLNGKNMTNSAASLATSPTSNQLNPK + 4 Deamidated (NQ)
881 634.3565 633.3492 633.3520 -4.32 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology		 KDIMK
1540 469.7159 937.4172 937.4327 -16.5 1 2 3 +1Score > 20 indicates identity GEMKSGWK + Oxidation (M)
2635 594.3177 1186.6208 1186.6193 1.28 1 2 0.65 +1Score > 22 indicates identity
Score > 13 indicates homology		 TSANPAEVAKAK + Deamidated (NQ)
1780 499.2333 996.4520 996.4552 -3.21 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology		 VSSWENFK + Deamidated (NQ)
3232 815.3859 1628.7572 1628.7352 13.5 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology		 DTSSQGMANKYSVPK + Deamidated (NQ); Oxidation (M)
3633 656.3643 1966.0711 1966.0385 16.6 1 2 2.5 +1Score > 19 indicates identity LWDARAVQLATTDLTHR
840 602.2794 601.2721 601.2707 2.31 0 2 1.2 +1Score > 15 indicates identity AGNDPK + Deamidated (NQ)
1892 511.2722 1020.5298 1020.5200 9.68 1 2 0.74 +1Score > 23 indicates identity
Score > 13 indicates homology		 LTSGSGSERK
2311 558.2610 1114.5074 1114.5155 -7.27 0 2 2.8 +1Score > 19 indicates identity INQWSSHSR + Deamidated (NQ)
1284 858.4153 857.4080 857.4171 -10.5 0 2 2 +1Score > 18 indicates identity YLSNIFT + Deamidated (NQ)
1109 386.2248 770.4350 770.4399 -6.25 1 2 2.1 +1Score > 18 indicates identity LQPRTR + Deamidated (NQ)
3395 879.4207 1756.8268 1756.8104 9.37 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 LGTHAHDSAMVWFGGR + Oxidation (M)
3921 1066.5071 2130.9996 2130.9779 10.2 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 LQMNLENEPLQDDFPEAK + Deamidated (NQ)
1332 875.4996 874.4923 874.4946 -2.58 1 2 0.82 +1Score > 21 indicates identity
Score > 14 indicates homology		 MKIELNK
2843 452.2327 1353.6763 1353.6818 -4.10 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 MSAMMVKLGLNK + 2 Oxidation (M)
1364 443.7432 885.4718 885.4742 -2.65 0 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 QMLIPQR + Deamidated (NQ)
836 598.3329 597.3256 597.3235 3.63 0 2 2.8 +1Score > 19 indicates identity GAPPTR
2543 390.1735 1167.4987 1167.5131 -12.4 1 2 1.9 +1Score > 17 indicates identity MSYYNRYR + Oxidation (M)
1019 722.3950 721.3877 721.3759 16.4 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 GTIFER
1097 766.3671 765.3598 765.3657 -7.69 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 LSDQFR + Deamidated (NQ)
3963 1076.5446 2151.0746 2151.0340 18.9 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 KAGGVVEATVCFSGDMLQPGK + Deamidated (NQ)
3407 882.9162 1763.8178 1763.8472 -16.6 1 2 0.87 +1Score > 21 indicates identity
Score > 14 indicates homology		 SPTKSSATNLAGMAEGAR + Oxidation (M)
4137 768.3825 2302.1257 2302.0972 12.4 1 2 0.99 +1Score > 22 indicates identity
Score > 14 indicates homology		 NNDAMEHTLGGGILTTSGSKQR + Oxidation (M)
3763 687.0266 2058.0580 2058.0204 18.3 0 2 0.8 +1Score > 20 indicates identity
Score > 13 indicates homology		 HCLQAVQNYIVQINVTR + 3 Deamidated (NQ)
1776 996.4827 995.4754 995.4780 -2.57 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 ISDMIMVR + 2 Oxidation (M)
1194 408.6964 815.3782 815.3813 -3.80 0 2 1.8 +1Score > 17 indicates identity VSYYER
1774 498.7432 995.4718 995.4560 16.0 0 2 0.68 +1Score > 21 indicates identity
Score > 13 indicates homology		 QGLEGNSYK + Deamidated (NQ)
2091 532.2684 1062.5222 1062.5346 -11.6 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 LGGYPDVSAGK
2582 1177.5897 1176.5824 1176.5775 4.20 0 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 GLPEGYSEGIR
2840 677.3415 1352.6684 1352.6460 16.6 1 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 GKESLQDVYDAK + Deamidated (NQ)
854 616.2932 615.2859 615.2864 -0.74 0 2 1.2 +1Score > 15 indicates identity QNQPK + 2 Deamidated (NQ)
890 639.3470 638.3397 638.3428 -4.82 0 2 0.82 +1Score > 20 indicates identity
Score > 13 indicates homology		 FTFPK
1034 728.3969 727.3896 727.3977 -11.1 0 2 1.8 +1Score > 17 indicates identity AVPGATGR
1955 517.7682 1033.5218 1033.5193 2.51 0 2 1 +1Score > 24 indicates identity
Score > 14 indicates homology		 QPFTVSNNK
2180 542.7596 1083.5046 1083.5019 2.57 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 RLQGNGMYK + 2 Deamidated (NQ); Oxidation (M)
2668 601.7924 1201.5702 1201.5761 -4.87 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 ANTPSSMHKTK + Deamidated (NQ)
3233 408.1970 1628.7589 1628.7431 9.72 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 QSSQGQYAVDSHPPK + Deamidated (NQ)
1337 877.4305 876.4232 876.4189 4.99 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 SLQDTANK + Deamidated (NQ)
2618 592.3647 1182.7148 1182.7012 11.5 1 2 1.2 +1Score > 15 indicates identity VKLAVLSYYK
791 531.2419 530.2346 530.2449 -19.3 0 2 0.69 +1Score > 13 indicates identity GPNSR + Deamidated (NQ)
1654 966.4833 965.4760 965.4818 -5.95 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 NIQGKNYK + 2 Deamidated (NQ)
3413 886.9830 1771.9514 1771.9832 -17.9 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 LLDVIINNLNNKNFK + Deamidated (NQ)
2796 656.8326 1311.6506 1311.6493 1.06 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 LGMTYATAELAR + Oxidation (M)
1232 420.7332 839.4518 839.4501 2.08 0 2 2.6 +1Score > 18 indicates identity NATPVNPK
1731 493.2526 984.4906 984.5062 -15.8 0 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 LMAIHQEK + Oxidation (M)
2200 544.7735 1087.5324 1087.5397 -6.64 0 2 0.9 +1Score > 22 indicates identity
Score > 14 indicates homology		 LNGQLNLEGK + 3 Deamidated (NQ)
4209 792.0466 2373.1180 2373.0893 12.1 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 ESTENMSLLNKEIQDLYDSK + Deamidated (NQ); Oxidation (M)
1827 504.2498 1006.4850 1006.4971 -12.0 0 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 GTNNVYPIK + 2 Deamidated (NQ)
4136 1152.0642 2302.1138 2302.1515 -16.4 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 LSMESPHVNVFSILTDLDIR + Deamidated (NQ); Oxidation (M)
2143 1076.5210 1075.5137 1075.5080 5.31 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 SLNSPNKCR + Deamidated (NQ)
3133 521.6019 1561.7839 1561.7997 -10.1 0 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 VLQTMSFLMNHLI + Oxidation (M)
3408 883.4112 1764.8078 1764.8240 -9.15 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 ETVHDNEMPLPQLPK + 2 Deamidated (NQ); Oxidation (M)
1340 440.2198 878.4250 878.4167 9.45 0 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 QLSEMQK + Oxidation (M)
4101 758.0237 2271.0493 2271.0390 4.54 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 TLQGIANQVDKTHNEQINDK + 6 Deamidated (NQ)
1110 386.2253 770.4360 770.4399 -4.97 0 2 2.5 +1Score > 18 indicates identity GAGGIGALR
4178 777.3740 2329.1002 2329.1260 -11.1 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 MSDNLLSLENPVVPSHYELR + Deamidated (NQ); Oxidation (M)
2163 1080.5225 1079.5152 1079.5247 -8.80 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 NENSVVYVR + Deamidated (NQ)
2795 655.8187 1309.6228 1309.6013 16.5 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 EYCPQFQPIK + Deamidated (NQ)
4391 922.4780 2764.4122 2764.4568 -16.2 0 2 0.85 +1Score > 22 indicates identity
Score > 13 indicates homology		 LVVDLISTSEVHVSMALPIPDADSLK + Oxidation (M)
1758 496.7443 991.4740 991.4644 9.71 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 IMDQLNNK + Deamidated (NQ); Oxidation (M)
2386 567.7829 1133.5512 1133.5452 5.35 0 2 0.84 +1Score > 22 indicates identity
Score > 13 indicates homology		 LVTDNSVEEK + Deamidated (NQ)
2751 633.8244 1265.6342 1265.6503 -12.7 1 2 0.91 +1Score > 22 indicates identity
Score > 14 indicates homology		 QVEKYIEQTK + Deamidated (NQ)
975 342.1960 682.3774 682.3762 1.82 1 1 2.1 +1Score > 17 indicates identity GPKEPR
1874 1018.4823 1017.4750 1017.4839 -8.71 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 SINENQRR + 2 Deamidated (NQ)
1791 500.2411 998.4676 998.4781 -10.5 0 1 3.6 +1Score > 20 indicates identity EGVGPNVGDR
2766 638.8337 1275.6528 1275.6684 -12.2 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 GTIQRNFNGIR + Deamidated (NQ)
3193 534.9479 1601.8219 1601.8382 -10.2 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 STVPIIIRCCIDR
1100 767.3691 766.3618 766.3531 11.4 1 1 0.8 +1Score > 21 indicates identity
Score > 13 indicates homology		 KDMQTK + Deamidated (NQ); Oxidation (M)
1279 855.4191 854.4118 854.3956 19.0 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 CYKDAAK
4444 960.4630 2878.3672 2878.3531 4.90 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 MREMFASSFSVKPGYTSPGYGAIHSR + Oxidation (M)
1165 800.3800 799.3727 799.3786 -7.31 0 1 1.9 +1Score > 17 indicates identity MYTQIK + Deamidated (NQ); Oxidation (M)
2019 524.7827 1047.5508 1047.5461 4.49 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 ARGVEVDFR
2333 560.7728 1119.5310 1119.5205 9.41 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 KSFLCMYR + Oxidation (M)
3018 749.3728 1496.7310 1496.7147 10.9 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 LDSEFQPLSSAFR + Deamidated (NQ)
4206 791.0631 2370.1675 2370.1226 18.9 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 EAFGIKEETNNFTSNELDALK + Deamidated (NQ)
3344 856.4327 1710.8508 1710.8797 -16.8 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 ISPLMKSQYNMVLR + 2 Oxidation (M)
2202 544.7740 1087.5334 1087.5397 -5.72 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 LNGQLNLEGK + 3 Deamidated (NQ)
2736 629.3210 1256.6274 1256.6071 16.2 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 NQMAHLVDLAK + 2 Deamidated (NQ); Oxidation (M)
2790 652.2998 1302.5850 1302.5728 9.40 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 GQFGQTNYSTAK + 2 Deamidated (NQ)
3856 525.2551 2096.9913 2097.0017 -4.96 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 VQTAVTMRQVSASMGQVCK + Deamidated (NQ); Oxidation (M)
1665 968.4799 967.4726 967.4545 18.7 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 FMEQKNR + Oxidation (M)
3410 884.4314 1766.8482 1766.8423 3.35 0 1 0.83 +1Score > 22 indicates identity
Score > 13 indicates homology		 SLNYLHHFANVCHR
3957 717.9930 2150.9572 2150.9603 -1.47 0 1 0.78 +1Score > 20 indicates identity
Score > 13 indicates homology		 NLVLTQSNFNGGINNDNNAK + 5 Deamidated (NQ)
3313 564.2995 1689.8767 1689.8542 13.3 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 KIICPSVESISNGMR
3140 782.8708 1563.7270 1563.7538 -17.1 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 SRHQFDLIMCLK + Deamidated (NQ); Oxidation (M)
1025 724.4112 723.4039 723.4140 -13.9 1 1 2.5 +1Score > 18 indicates identity GNKHLR
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