MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4401–4500 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4395 925.5075 2773.5007
4396 925.5131 2773.5175
4397 926.1754 2775.5044
4399 927.8002 2780.3788
4400 927.8345 2780.4817
4401 928.4521 2782.3345
4402 929.4632 2785.3678
4403 929.4742 2785.4008
4404 929.5006 2785.4800
4405 698.1224 2788.4605
4406 698.1235 2788.4649
4407 558.8991 2789.4591
4408 931.1857 2790.5353
4409 931.4548 2791.3426
4410 932.1270 2793.3592
4411 932.4567 2794.3483
4412 932.7789 2795.3149
4413 933.1147 2796.3223
4414 933.4644 2797.3714
4415 933.8035 2798.3887
4417 702.8929 2807.5425
4418 703.1407 2808.5337
4419 938.4457 2812.3153
4420 938.4573 2812.3501
4421 938.4696 2812.3870
4423 942.1295 2823.3667
4424 942.4669 2824.3789
4425 943.4579 2827.3519
4426 943.7823 2828.3251
4427 943.7881 2828.3425
4428 945.4355 2833.2847
4429 945.8101 2834.4085
4430 947.4576 2839.3510
4431 710.8553 2839.3921
4432 947.4766 2839.4080
4433 948.1698 2841.4876
4434 949.1180 2844.3322
4435 950.7606 2849.2600
4436 951.4639 2851.3699
4437 951.4741 2851.4005
4438 952.1246 2853.3520
4439 714.3602 2853.4117
4441 957.4598 2869.3576
4442 957.4702 2869.3888
4443 957.4807 2869.4203
4445 960.8188 2879.4346
4446 962.7916 2885.3530
4447 962.8120 2885.4142
4449 965.5178 2893.5316
4450 967.4770 2899.4092
4451 968.4570 2902.3492
4452 971.4892 2911.4458
4453 972.0976 2913.2710
4454 973.7842 2918.3308
4456 974.2022 2919.5848
4458 975.4669 2923.3789
4459 975.4670 2923.3792
4460 975.4682 2923.3828
4461 975.4861 2923.4365
4462 975.8227 2924.4463
4463 980.4608 2938.3606
4464 980.7955 2939.3647
4465 980.7993 2939.3761
4466 981.1534 2940.4384
4467 981.1637 2940.4693
4468 981.4673 2941.3801
4469 736.3743 2941.4681
4470 982.4679 2944.3819
4471 982.4807 2944.4203
4472 982.4808 2944.4206
4473 982.7863 2945.3371
4475 983.4542 2947.3408
4476 984.4963 2950.4671
4477 738.8729 2951.4625
4479 985.8158 2954.4256
4480 986.1290 2955.3652
4481 986.4618 2956.3636
4482 986.4908 2956.4506
4483 986.7991 2957.3755
4484 986.8215 2957.4427
4485 740.3704 2957.4525
4486 988.1125 2961.3157
4487 988.4447 2962.3123
4488 988.4589 2962.3549
4489 989.8302 2966.4688
4490 989.8329 2966.4769
4491 990.1584 2967.4534
4492 990.1609 2967.4609
4493 742.8732 2967.4637
4494 742.8741 2967.4673
4495 742.8792 2967.4877
4497 990.4807 2968.4203
4498 990.4946 2968.4620
4499 990.5220 2968.5442
4500 991.8226 2972.4460
4501 744.3699 2973.4505
4502 993.4427 2977.3063
4504 994.5130 2980.5172
4505 994.8220 2981.4442
4506 746.3714 2981.4565
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