MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4301–4400 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4288 835.7581 2504.2525
4289 837.7267 2510.1583
4290 837.7790 2510.3152
4291 838.0639 2511.1699
4292 838.1437 2511.4093
4293 838.3816 2512.1230
4294 838.4224 2512.2454
4295 838.4240 2512.2502
4296 839.0372 2514.0898
4297 630.0879 2516.3225
4298 839.7834 2516.3284
4299 842.4036 2524.1890
4300 843.0565 2526.1477
4301 632.5976 2526.3613
4302 843.3655 2527.0747
4303 843.7505 2528.2297
4304 843.7538 2528.2396
4305 843.7549 2528.2429
4306 845.4024 2533.1854
4307 846.4780 2536.4122
4308 847.7267 2540.1583
4309 849.0868 2544.2386
4310 849.4217 2545.2433
4311 849.4242 2545.2508
4312 851.3851 2551.1335
4313 853.4241 2557.2505
4314 854.7119 2561.1139
4315 856.1284 2565.3634
4316 857.4263 2569.2571
4317 857.4286 2569.2640
4318 858.0565 2571.1477
4319 860.4120 2578.2142
4320 861.4162 2581.2268
4322 862.7615 2585.2627
4323 863.3840 2587.1302
4324 863.3944 2587.1614
4325 864.8245 2591.4517
4326 866.0742 2595.2008
4327 866.4189 2596.2349
4328 866.7362 2597.1868
4329 867.0912 2598.2518
4330 867.0914 2598.2524
4331 869.0866 2604.2380
4332 869.4149 2605.2229
4333 652.8077 2607.2017
4334 870.4637 2608.3693
4336 872.0638 2613.1696
4337 872.0657 2613.1753
4338 872.4202 2614.2388
4339 873.0939 2616.2599
4340 876.4065 2626.1977
4341 876.4108 2626.2106
4342 876.4325 2626.2757
4343 877.0768 2628.2086
4344 877.4224 2629.2454
4345 882.0607 2643.1603
4346 882.0698 2643.1876
4347 882.4321 2644.2745
4348 883.4365 2647.2877
4349 883.4738 2647.3996
4350 883.4748 2647.4026
4352 886.4308 2656.2706
4353 891.4271 2671.2595
4354 891.4308 2671.2706
4355 891.7760 2672.3062
4356 895.4430 2683.3072
4357 672.3434 2685.3445
4358 896.4480 2686.3222
4359 896.4644 2686.3714
4360 898.4488 2692.3246
4362 898.7781 2693.3125
4363 898.8455 2693.5147
4364 899.4218 2695.2436
4365 899.4348 2695.2826
4366 901.4376 2701.2910
4367 904.7845 2711.3317
4368 907.4243 2719.2511
4369 907.7906 2720.3500
4370 908.4656 2722.3750
4371 683.3228 2729.2621
4372 912.4583 2734.3531
4373 912.4688 2734.3846
4374 913.4623 2737.3651
4375 915.1065 2742.2977
4376 915.1458 2742.4156
4377 915.1506 2742.4300
4378 688.6331 2750.5033
4379 917.8430 2750.5072
4380 917.8436 2750.5090
4381 688.6361 2750.5153
4382 918.1729 2751.4969
4383 918.4311 2752.2715
4384 919.4438 2755.3096
4385 689.8419 2755.3385
4387 921.0983 2760.2731
4388 922.1368 2763.3886
4389 922.1411 2763.4015
4392 922.8350 2765.4832
4393 923.7828 2768.3266
4394 925.1686 2772.4840
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