MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4177 1165.5564 2329.0982
4180 777.3811 2329.1215
4181 1168.5529 2335.0912
4182 779.6992 2336.0758
4183 780.4003 2338.1791
4184 1170.5955 2339.1764
4185 781.3674 2341.0804
4186 782.7060 2345.0962
4187 587.5263 2346.0761
4188 783.3691 2347.0855
4189 783.6843 2348.0311
4190 784.4006 2350.1800
4191 784.6905 2351.0497
4192 784.7014 2351.0824
4193 1176.5525 2351.0904
4194 785.7072 2354.0998
4195 787.3704 2359.0894
4197 789.3861 2365.1365
4198 1183.5787 2365.1428
4199 789.3904 2365.1494
4200 1183.5834 2365.1522
4201 789.7105 2366.1097
4202 789.7214 2366.1424 2366.1311 4.76 1     +0Score > 23 indicates identity
Score > 13 indicates homology		 AVDNAPERYQAILDSSLMVEK + 2 Deamidated (NQ); Oxidation (M)
4203 1184.5454 2367.0762
4204 790.0332 2367.0778
4205 593.5475 2370.1609
4207 791.3849 2371.1329
4208 1187.5646 2373.1146
4210 794.0746 2379.2020
4211 794.3876 2380.1410
4212 1191.1044 2380.1942
4213 794.4113 2380.2121
4214 1191.5729 2381.1312
4215 794.7211 2381.1415
4216 1191.5829 2381.1512
4217 795.0310 2382.0712
4218 796.3727 2386.0963
4219 796.6882 2387.0428
4221 797.4397 2389.2973
4222 799.7361 2396.1865
4223 800.3904 2398.1494
4224 800.7253 2399.1541
4225 803.7080 2408.1022
4226 804.3757 2410.1053
4227 805.1267 2412.3583
4228 805.3580 2413.0522
4229 805.7233 2414.1481
4230 604.8116 2415.2173
4232 807.3827 2419.1263
4234 809.3959 2425.1659
4235 809.3968 2425.1686
4236 608.8110 2431.2149
4237 811.4202 2431.2388
4238 811.6967 2432.0683
4239 813.4156 2437.2250
4240 813.7517 2438.2333
4241 813.7852 2438.3338
4242 814.0684 2439.1834
4243 814.3727 2440.0963
4244 814.3912 2440.1518
4245 814.7289 2441.1649
4246 816.7507 2447.2303
4247 817.1365 2448.3877
4248 819.0386 2454.0940
4249 819.3987 2455.1743
4250 819.4001 2455.1785
4251 819.4163 2455.2271
4252 819.4169 2455.2289
4253 819.4172 2455.2298
4254 820.0601 2457.1585
4255 821.4005 2461.1797
4256 822.1086 2463.3040
4257 822.7898 2465.3476
4258 823.7402 2468.1988
4259 824.0344 2469.0814
4260 618.3434 2469.3445
4261 824.4173 2470.2301
4262 824.7481 2471.2225
4263 618.8129 2471.2225
4264 824.7483 2471.2231
4265 825.3931 2473.1575
4266 826.3821 2476.1245
4267 826.7178 2477.1316
4268 827.1362 2478.3868
4269 827.4291 2479.2655
4270 828.4049 2482.1929
4271 828.4145 2482.2217
4272 828.7333 2483.1781
4273 829.3928 2485.1566
4274 829.4141 2485.2205
4275 829.7537 2486.2393
4277 622.8111 2487.2153
4278 830.0800 2487.2182
4279 830.4191 2488.2355
4281 832.0506 2493.1300
4282 832.4015 2494.1827
4283 832.4217 2494.2433
4285 834.0410 2499.1012
4286 834.3847 2500.1323
4287 834.7921 2501.3545
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