MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4061 747.3365 2238.9877
4062 1120.5266 2239.0386
4063 1120.9921 2239.9696
4064 748.9940 2243.9602
4065 750.0867 2247.2383
4066 750.3778 2248.1116
4067 1125.5122 2249.0098
4068 750.7044 2249.0914
4069 1126.0597 2250.1048
4070 751.0427 2250.1063
4071 751.0513 2250.1321
4072 751.3718 2251.0936
4073 1126.5549 2251.0952
4074 751.3773 2251.1101
4075 1126.5645 2251.1144
4076 751.3799 2251.1179
4077 751.3809 2251.1209
4078 751.7773 2252.3101
4079 752.0103 2253.0091
4080 1127.5140 2253.0134
4081 1127.5164 2253.0182
4082 752.0134 2253.0184
4083 752.3765 2254.1077
4084 1128.5607 2255.1068
4085 1129.5244 2257.0342
4086 1130.5461 2259.0776
4087 1130.5463 2259.0780
4088 1131.5520 2261.0894
4089 754.7054 2261.0944
4090 755.0522 2262.1348
4091 1133.5551 2265.0956
4092 756.3732 2266.0978
4093 1134.0568 2266.0990
4094 756.3768 2266.1086
4095 1134.5592 2267.1038
4096 1135.5120 2269.0094
4097 1135.5402 2269.0658
4098 757.6738 2269.9996
4099 1136.5063 2270.9980
4100 1136.5282 2271.0418
4102 1136.5441 2271.0736
4103 1136.5511 2271.0876
4104 758.3668 2272.0786
4105 1138.3528 2274.6910
4106 1138.5441 2275.0736
4107 760.3815 2278.1227
4108 1140.5583 2279.1020
4109 760.7157 2279.1253
4110 1144.5896 2287.1646
4112 1146.5695 2291.1244
4113 764.7196 2291.1370
4114 1146.5778 2291.1410
4115 765.0497 2292.1273
4117 765.4165 2293.2277
4118 765.4183 2293.2331
4119 765.4198 2293.2376
4120 765.4209 2293.2409
4121 765.4224 2293.2454
4122 765.4230 2293.2472
4123 765.4235 2293.2487
4124 765.4250 2293.2532
4125 765.4254 2293.2544
4126 765.4289 2293.2649
4129 1149.5197 2297.0248
4130 766.6998 2297.0776
4131 1149.5708 2297.1270
4132 1150.0614 2298.1082
4133 767.3539 2299.0399
4135 1151.5740 2301.1334
4140 768.3923 2302.1551
4141 768.7222 2303.1448
4142 1152.5815 2303.1484
4144 1153.5829 2305.1512
4145 769.3942 2305.1608
4147 770.0709 2307.1909
4148 770.3819 2308.1239
4150 1155.0697 2308.1248
4151 770.3823 2308.1251
4152 770.3827 2308.1263
4153 1155.0726 2308.1306
4154 770.3865 2308.1377
4155 1156.0460 2310.0774
4156 1157.5613 2313.1080
4157 772.0446 2313.1120
4158 1157.5638 2313.1130
4159 772.3663 2314.0771
4160 772.3820 2314.1242
4161 773.3900 2317.1482
4162 774.0638 2319.1696
4163 775.3820 2323.1242
4165 775.4020 2323.1842
4166 1162.6021 2323.1896
4167 1163.0670 2324.1194
4168 1163.0671 2324.1196
4171 1163.0697 2324.1248
4172 1163.5463 2325.0780
4173 1163.5565 2325.0984
4174 776.4103 2326.2091
4175 777.3668 2329.0786
4176 1165.5564 2329.0982
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