MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3949 715.3611 2143.0615
3950 715.7342 2144.1808
3951 716.6400 2146.8982
3952 1074.4967 2146.9788
3953 1074.5095 2147.0044
3954 717.3350 2148.9832
3955 1075.4991 2148.9836
3956 1076.0193 2150.0240
3958 1076.4871 2150.9596
3959 1076.5261 2151.0376
3960 718.0202 2151.0388
3961 718.0215 2151.0427
3962 1076.5386 2151.0626
3964 718.3643 2152.0711
3965 1077.4839 2152.9532
3966 1078.5101 2155.0056
3967 1078.5106 2155.0066
3968 1078.9768 2155.9390
3969 1079.4805 2156.9464
3970 1079.5156 2157.0166
3971 720.6508 2158.9306
3972 1080.9935 2159.9724
3973 1081.5105 2161.0064
3974 1082.0544 2162.0942
3975 721.7067 2162.0983
3976 1082.4995 2162.9844
3979 722.6648 2164.9726
3980 722.6655 2164.9747
3981 1083.4958 2164.9770
3982 722.6774 2165.0104
3983 1083.5126 2165.0106
3984 1083.5171 2165.0196
3985 1084.5071 2166.9996
3986 1085.4833 2168.9520
3987 1085.5314 2169.0482
3988 1085.5603 2169.1060
3990 1086.5286 2171.0426
3991 1086.5415 2171.0684
3992 1087.4575 2172.9004
3993 725.3129 2172.9169
3994 725.3459 2173.0159
3995 725.6854 2174.0344
3996 1088.5667 2175.1188
3997 726.3563 2176.0471
4000 1089.5614 2177.1082
4001 1090.5289 2179.0432
4003 1091.5453 2181.0760
4004 1091.9956 2181.9766
4005 1091.9979 2181.9812
4006 729.0859 2184.2359
4007 1093.5425 2185.0704
4008 1093.6268 2185.2390
4009 1094.5020 2186.9894
4012 1097.0573 2192.1000
4013 731.7102 2192.1088
4014 1097.9969 2193.9792
4015 732.3357 2193.9853
4016 1098.0067 2193.9988
4017 1098.9982 2195.9818
4018 1099.0016 2195.9886
4019 733.0072 2195.9998
4020 1099.5411 2197.0676
4021 1100.4916 2198.9686
4022 1101.5309 2201.0472
4023 734.6983 2201.0731
4024 1101.5444 2201.0742
4025 1101.5465 2201.0784
4026 734.7404 2201.1994
4029 736.7028 2207.0866
4030 1105.0369 2208.0592
4031 737.0280 2208.0622
4032 737.3874 2209.1404
4033 1105.9960 2209.9774
4034 738.0022 2210.9848
4035 1107.0013 2211.9880
4036 1107.4886 2212.9626
4037 1107.5420 2213.0694
4038 738.9936 2213.9590
4039 1108.4897 2214.9648
4040 1108.5117 2215.0088
4041 739.6542 2215.9408
4042 1108.9792 2215.9438
4043 1109.5284 2217.0422
4044 1110.0325 2218.0504
4045 740.3603 2218.0591
4046 740.9846 2219.9320
4047 1111.5223 2221.0300
4048 1111.5258 2221.0370
4049 1112.4972 2222.9798
4050 743.3560 2227.0462
4051 1114.5322 2227.0498
4052 1115.9851 2229.9556
4053 744.6586 2230.9540
4054 1117.5259 2233.0372
4055 745.6801 2234.0185
4056 1118.0177 2234.0208
4057 746.6738 2236.9996
4058 746.6814 2237.0224
4059 1119.5253 2237.0360
4060 1120.4988 2238.9830
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