MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4764)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3844 1045.9893 2089.9640
3845 1045.9900 2089.9654
3846 698.3269 2091.9589
3847 1046.9873 2091.9600
3848 1047.4921 2092.9696
3849 698.9631 2093.8675
3850 698.9726 2093.8960
3851 524.4935 2093.9449
3852 1048.4730 2094.9314
3853 699.3208 2094.9406
3854 699.6691 2095.9855
3855 700.0027 2096.9863
3857 1049.5225 2097.0304
3858 1049.5345 2097.0544
3859 700.3345 2097.9817
3860 1049.9985 2097.9824
3861 1050.0011 2097.9876
3862 1050.5060 2098.9974
3863 1050.5140 2099.0134
3864 1050.5217 2099.0288
3865 1051.0189 2100.0232
3866 701.0161 2100.0265
3867 701.3382 2100.9928
3868 1051.5043 2100.9940
3869 701.9990 2102.9752
3870 1052.4979 2102.9812
3871 702.3347 2103.9823
3872 1053.4801 2104.9456
3873 1053.4878 2104.9610
3874 1053.4932 2104.9718
3875 1053.9882 2105.9618
3876 702.9946 2105.9620
3877 703.6569 2107.9489
3878 1055.4813 2108.9480
3879 1055.4830 2108.9514
3880 703.9924 2108.9554
3881 1055.5101 2109.0056
3882 1055.5112 2109.0078
3883 1055.5204 2109.0262
3884 1056.4489 2110.8832
3885 1056.4673 2110.9200
3886 1057.4952 2112.9758
3888 1057.5067 2112.9988
3889 1057.5068 2112.9990
3890 1057.5084 2113.0022
3891 705.3472 2113.0198
3892 1057.5172 2113.0198
3893 1057.5175 2113.0204
3894 1057.5184 2113.0222
3895 1057.5188 2113.0230
3896 705.6871 2114.0395
3897 1058.5100 2115.0054
3898 1058.5103 2115.0060
3899 1058.5826 2115.1506
3900 706.0585 2115.1537
3901 1059.5106 2117.0066
3902 706.9980 2117.9722
3903 1060.4934 2118.9722
3904 1060.4937 2118.9728
3905 707.3320 2118.9742
3906 707.6606 2119.9600
3908 1061.4911 2120.9676
3909 1061.9869 2121.9592
3910 708.6854 2123.0344
3911 1062.5272 2123.0398
3912 1062.5309 2123.0472
3913 1063.0083 2124.0020
3915 1063.5299 2125.0452
3916 1065.5117 2129.0088
3917 1065.9689 2129.9232
3918 1066.0157 2130.0168
3919 1066.4958 2130.9770
3920 711.3375 2130.9907
3922 1066.5128 2131.0110
3923 1066.5167 2131.0188
3924 1066.5183 2131.0220
3925 1067.5029 2132.9912
3926 712.0051 2132.9935
3927 712.0200 2133.0382
3928 1067.5309 2133.0472
3929 712.3479 2134.0219
3930 1068.0217 2134.0288
3931 712.6627 2134.9663
3932 1068.4923 2134.9700
3933 712.6646 2134.9720
3934 1068.5082 2135.0018
3935 712.6906 2135.0500
3936 1068.5410 2135.0674
3937 712.6977 2135.0713
3938 712.9884 2135.9434
3939 1069.0087 2136.0028
3940 1069.4856 2136.9566
3941 1069.4858 2136.9570
3942 1069.5076 2137.0006
3943 1069.5117 2137.0088
3944 1069.5155 2137.0164
3945 713.6824 2138.0254
3946 1071.4929 2140.9712
3947 1072.5094 2143.0042
3948 715.3594 2143.0564
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