MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4764)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2621 592.7635 1183.5124 1183.5292 -14.1 0 3 1.9 +1Score > 19 indicates identity QSQFNLMSGR + Deamidated (NQ); Oxidation (M)
2533 583.2847 1164.5548 1164.5676 -10.9 0 3 0.69 +1Score > 23 indicates identity
Score > 14 indicates homology
NNLHAEGWPK
952 337.1799 672.3452 672.3555 -15.2 1 3 1.8 +1Score > 19 indicates identity EPGKSR
2756 635.8480 1269.6814 1269.6573 19.0 0 3 0.49 +1Score > 20 indicates identity
Score > 13 indicates homology
VPHMCALSLVK + Oxidation (M)
1756 496.2661 990.5176 990.5056 12.2 0 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
AMGINDLLK + Deamidated (NQ); Oxidation (M)
3217 810.4075 1618.8004 1618.7739 16.4 0 3 0.53 +1Score > 23 indicates identity
Score > 13 indicates homology
LITAYYNEHGPGQR + Deamidated (NQ)
4390 922.4689 2764.3849 2764.3959 -3.99 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
QQAAETKELQEIPPQLLFVHWQK + 4 Deamidated (NQ)
3122 778.8516 1555.6886 1555.6613 17.6 0 3 0.97 +1Score > 21 indicates identity
Score > 16 indicates homology
EHMSFEDYSGNLK
1036 729.3427 728.3354 728.3381 -3.70 0 3 0.78 +1Score > 15 indicates identity TSLDFF
1319 434.7182 867.4218 867.4239 -2.35 0 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
HNSWVPK + Deamidated (NQ)
3175 530.9440 1589.8102 1589.7970 8.27 1 3 0.65 +1Score > 22 indicates identity
Score > 14 indicates homology
EAQRIEGMLQLLTS + 2 Deamidated (NQ)
3466 912.4131 1822.8116 1822.8155 -2.13 1 3 2.3 +1Score > 19 indicates identity NNFKIIDSHSMNNSGK + 2 Deamidated (NQ); Oxidation (M)
2391 1137.5398 1136.5325 1136.5132 17.0 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
AAERDNMLTT + Oxidation (M)
1153 794.3692 793.3619 793.3640 -2.61 0 3 0.66 +1Score > 21 indicates identity
Score > 14 indicates homology
QLGDSMK + Oxidation (M)
899 641.3636 640.3563 640.3657 -14.6 0 3 0.97 +1Score > 16 indicates identity VGVNPR
1471 460.7159 919.4172 919.4247 -8.05 1 3 0.92 +1Score > 21 indicates identity
Score > 15 indicates homology
QNESEGKK + Deamidated (NQ)
3275 830.8858 1659.7570 1659.7410 9.68 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
KSHNMPSSILDDGNK + 2 Deamidated (NQ); Oxidation (M)
2398 570.2716 1138.5286 1138.5506 -19.3 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
AGDKYSIEQK + Deamidated (NQ)
3302 842.9010 1683.7874 1683.7661 12.7 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
QIQEYESMNGNLIK + 2 Deamidated (NQ); Oxidation (M)
980 345.1771 688.3396 688.3293 15.1 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
SFNGHK
1257 848.3794 847.3721 847.3671 5.88 0 3 2.4 +1Score > 20 indicates identity LGNANSDR + 2 Deamidated (NQ)
865 623.3515 622.3442 622.3551 -17.5 1 3 0.87 +1Score > 22 indicates identity
Score > 15 indicates homology
HGKGPK
4457 975.1677 2922.4813 2922.4572 8.24 1 3 1 +1Score > 20 indicates identity
Score > 16 indicates homology
ISFLQSKLQQFATSSMETYILEQTK + 2 Deamidated (NQ)
3397 879.8848 1757.7550 1757.7380 9.69 0 3 1.7 +1Score > 18 indicates identity STSPNANANDNTEFFK + 2 Deamidated (NQ)
2727 625.3329 1248.6512 1248.6714 -16.1 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
LGIKQDNSVFK + Deamidated (NQ)
2967 487.2389 1458.6949 1458.7177 -15.6 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
MTTFRPLSSFEK + Oxidation (M)
1021 361.7016 721.3886 721.3911 -3.46 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
FLTWR
3071 764.3559 1526.6972 1526.7221 -16.3 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
GNSMLFTMNRDIK + Deamidated (NQ)
2821 667.8414 1333.6682 1333.6547 10.1 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
VENGIMQSISEK
3051 757.9140 1513.8134 1513.8075 3.94 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
KPTMIKLQFHNR + 2 Deamidated (NQ)
4231 806.1066 2415.2980 2415.2686 12.2 1 3 1.9 +1Score > 18 indicates identity AIFPNTVDGDLLKLVHLSNGYK + 2 Deamidated (NQ)
1782 499.2336 996.4526 996.4400 12.7 0 3 0.84 +1Score > 20 indicates identity
Score > 15 indicates homology
ADSFINNSK + 2 Deamidated (NQ)
3735 1020.9894 2039.9642 2039.9946 -14.9 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
NIGVADPQNSLNPSKPNMK + Deamidated (NQ); Oxidation (M)
2052 1055.5127 1054.5054 1054.5083 -2.77 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
SINHPPDFK + Deamidated (NQ)
1022 723.3326 722.3253 722.3269 -2.15 0 3 2.3 +1Score > 19 indicates identity MSDNLK + Oxidation (M)
3690 669.0362 2004.0868 2004.0568 15.0 1 3 1.9 +1Score > 18 indicates identity LPPLRFLDDYIEISADK
1496 928.4130 927.4057 927.4158 -10.9 1 3 1.6 +1Score > 17 indicates identity RTQNDHR + 2 Deamidated (NQ)
851 612.3359 611.3286 611.3391 -17.1 0 3 0.74 +1Score > 14 indicates identity KPDPR
857 618.2739 617.2666 617.2769 -16.6 0 3 0.59 +1Score > 13 indicates identity QQGER + Deamidated (NQ)
1286 858.4194 857.4121 857.4171 -5.77 0 3 1.9 +1Score > 18 indicates identity YLSNIFT + Deamidated (NQ)
1189 814.3699 813.3626 813.3691 -7.94 0 3 1.3 +1Score > 17 indicates identity TYVQMR + Deamidated (NQ); Oxidation (M)
2260 551.2820 1100.5494 1100.5713 -19.9 1 3 0.79 +1Score > 21 indicates identity
Score > 14 indicates homology
TIEPQEEKK
852 613.2957 612.2884 612.2980 -15.6 0 3 1 +1Score > 16 indicates identity NHSGAK
2560 586.7639 1171.5132 1171.5357 -19.1 0 3 1.2 +1Score > 16 indicates identity IGGVNNPEQNK + 3 Deamidated (NQ)
3056 759.4263 1516.8380 1516.8110 17.8 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
LSSASGLNVPGGFRR
3076 765.9091 1529.8036 1529.8300 -17.3 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
KISQLVQENVTLR + 3 Deamidated (NQ)
1391 448.2366 894.4586 894.4672 -9.52 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QPGHLTSR
1092 762.3441 761.3368 761.3378 -1.24 0 3 2.3 +1Score > 19 indicates identity ENSGPMK
1143 787.3635 786.3562 786.3508 6.94 0 3 2.1 +1Score > 18 indicates identity NDVAPNR + 2 Deamidated (NQ)
4143 1152.5822 2303.1498 2303.1501 -0.096 1 3 0.9 +1Score > 22 indicates identity
Score > 15 indicates homology
LQQLSESIKITEMQLNHMK + Deamidated (NQ); 2 Oxidation (M)
3542 940.4294 1878.8442 1878.8451 -0.46 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
VRMQGEEQENIMIER + 2 Deamidated (NQ); Oxidation (M)
1783 997.4606 996.4533 996.4552 -1.93 0 3 0.71 +1Score > 20 indicates identity
Score > 14 indicates homology
VSSWENFK + Deamidated (NQ)
896 321.1848 640.3550 640.3657 -16.6 0 3 1.1 +1Score > 16 indicates identity VGVNPR
3747 1024.9808 2047.9470 2047.9772 -14.7 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
PGQIISIPFLSQNEDMDK + Deamidated (NQ); Oxidation (M)
2385 1134.5542 1133.5469 1133.5564 -8.34 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
QLSSKQNAQK + 3 Deamidated (NQ)
2814 664.8308 1327.6470 1327.6408 4.71 0 3 0.61 +1Score > 22 indicates identity
Score > 13 indicates homology
NSATILNYFER + Deamidated (NQ)
2345 1123.5383 1122.5310 1122.5305 0.45 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
SLEDRFNNK + Deamidated (NQ)
2725 624.3267 1246.6388 1246.6193 15.6 0 3 0.61 +1Score > 23 indicates identity
Score > 13 indicates homology
AGILNDAWATSK + Deamidated (NQ)
3636 984.4813 1966.9480 1966.9346 6.84 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
ANPNPVKFMNTSEIFQK + 3 Deamidated (NQ)
2194 544.2862 1086.5578 1086.5709 -12.0 0 3 0.76 +1Score > 22 indicates identity
Score > 14 indicates homology
EHTIIAEFK
2797 656.8348 1311.6550 1311.6744 -14.8 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
DVKSQISMIFK + Deamidated (NQ); Oxidation (M)
3196 801.9228 1601.8310 1601.8382 -4.44 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
STVPIIIRCCIDR
4676 1167.5460 3499.6162 3499.6518 -10.2 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
YPSLLDDSFITLTNREIDDYSDLPSPNPSSK + Deamidated (NQ)
2239 1096.5283 1095.5210 1095.5271 -5.50 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
ISDFVCLDK
2714 619.7969 1237.5792 1237.5575 17.6 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
NPESPESVHNK + Deamidated (NQ)
2769 639.3300 1276.6454 1276.6636 -14.2 1 3 0.84 +1Score > 23 indicates identity
Score > 14 indicates homology
SPTNNNPRIHK
1698 490.2278 978.4410 978.4328 8.43 0 3 2.4 +1Score > 19 indicates identity ADSPMQSVK + Deamidated (NQ); Oxidation (M)
3998 726.3758 2176.1056 2176.0899 7.19 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
STPFPYLTSSTSSSSLASTKK
4179 777.3784 2329.1134 2329.1260 -5.41 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
MSDNLLSLENPVVPSHYELR + Deamidated (NQ); Oxidation (M)
3404 880.8611 1759.7076 1759.7384 -17.5 0 3 0.88 +1Score > 15 indicates identity TGNEDVGNNNPSNSIPK + 4 Deamidated (NQ)
2087 1062.5289 1061.5216 1061.5426 -19.8 0 3 0.81 +1Score > 22 indicates identity
Score > 14 indicates homology
LNSELLMNK + Deamidated (NQ)
2535 583.2970 1164.5794 1164.5622 14.8 0 3 0.84 +1Score > 23 indicates identity
Score > 14 indicates homology
ITEGSSAASNTK
2918 718.3619 1434.7092 1434.7354 -18.2 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
TSPIVALTAQNYR + 2 Deamidated (NQ)
3022 750.8644 1499.7142 1499.7157 -0.98 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
YHSHNDTIFIPR + Deamidated (NQ)
877 315.6645 629.3144 629.3133 1.86 0 3 1.2 +1Score > 16 indicates identity TAQPGR + Deamidated (NQ)
3153 789.8978 1577.7810 1577.7533 17.6 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
ERDGSTEETLNSLK
3168 530.2738 1587.7996 1587.7715 17.7 0 3 0.67 +1Score > 24 indicates identity
Score > 13 indicates homology
MHDLNIPTFSSIGR + Deamidated (NQ)
1891 1021.5353 1020.5280 1020.5200 7.89 1 3 0.58 +1Score > 23 indicates identity
Score > 13 indicates homology
LTSGSGSERK
4128 766.4274 2296.2604 2296.2175 18.7 1 3 1.6 +1Score > 17 indicates identity IQHHITETILTYASLNKGTR + Deamidated (NQ)
882 634.3584 633.3511 633.3520 -1.32 1 3 0.95 +1Score > 23 indicates identity
Score > 15 indicates homology
DKLMK
3106 516.2711 1545.7915 1545.8012 -6.27 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
VASHKNSHNPSLVR + Deamidated (NQ)
3558 632.9946 1895.9620 1895.9299 16.9 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
DGALLCGILDKSQYGASK + Deamidated (NQ)
2669 602.8026 1203.5906 1203.5731 14.6 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
GLNVSNNQISR + 3 Deamidated (NQ)
3403 880.8589 1759.7032 1759.7384 -20.0 0 2 0.76 +1Score > 14 indicates identity TGNEDVGNNNPSNSIPK + 4 Deamidated (NQ)
4440 952.1475 2853.4207 2853.4648 -15.4 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
EYVSLVGQVLLDGSSLSNEEILQFSK
4138 768.3841 2302.1305 2302.1297 0.34 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
LIHMERDLSSEVVGQMDAIK + 2 Oxidation (M)
3097 769.8656 1537.7166 1537.7233 -4.32 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
RPDNQNPEGENLR
3057 759.8602 1517.7058 1517.7361 -20.0 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
NLDAEDESLAKWK
3664 993.4861 1984.9576 1984.9313 13.3 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
DVQYDAACADLRISFNK
4134 1151.5692 2301.1238 2301.1675 -19.0 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
LSMESPHVNVFSILTDLDIR + Oxidation (M)
4474 982.8130 2945.4172 2945.4440 -9.11 1 2 0.7 +1Score > 22 indicates identity
Score > 13 indicates homology
SLGLDVPRPMQVDFSNTLQKNADALGR + 4 Deamidated (NQ)
1533 468.7134 935.4122 935.4196 -7.86 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
GTAGSGETEK
3375 872.3660 1742.7174 1742.7192 -1.03 0 2 1.4 +1Score > 16 indicates identity EGVLMEDEGNEEYTK + Deamidated (NQ)
2015 1048.5442 1047.5369 1047.5309 5.77 0 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
VGSIGSQSASR
1673 485.7467 969.4788 969.4879 -9.37 0 2 2.7 +1Score > 19 indicates identity APAGGAADAAAK
2476 1154.5648 1153.5575 1153.5657 -7.10 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
MNMKMLLTR + Deamidated (NQ); Oxidation (M)
888 639.3467 638.3394 638.3428 -5.29 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
FTFPK
1185 406.2207 810.4268 810.4348 -9.79 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
VSPNAAPR
3300 842.4288 1682.8430 1682.8726 -17.6 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
NAINPLQTNVQILNK + 4 Deamidated (NQ)
3001 742.8782 1483.7418 1483.7605 -12.6 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
IDQLNWKMTHAK
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