| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1245_Other_P1_B10.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:21:38 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,780 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 592.7635 | 1183.5124 | 1183.5292 | -14.1 | 0 | 3 | 1.9 | 1Score > 19 indicates identity |
QSQFNLMSGR + Deamidated (NQ); Oxidation (M) | |
| 583.2847 | 1164.5548 | 1164.5676 | -10.9 | 0 | 3 | 0.69 | 1Score > 23 indicates identityScore > 14 indicates homology |
NNLHAEGWPK | |
| 337.1799 | 672.3452 | 672.3555 | -15.2 | 1 | 3 | 1.8 | 1Score > 19 indicates identity |
EPGKSR | |
| 635.8480 | 1269.6814 | 1269.6573 | 19.0 | 0 | 3 | 0.49 | 1Score > 20 indicates identityScore > 13 indicates homology |
VPHMCALSLVK + Oxidation (M) | |
| 496.2661 | 990.5176 | 990.5056 | 12.2 | 0 | 3 | 1 | 1Score > 23 indicates identityScore > 16 indicates homology |
AMGINDLLK + Deamidated (NQ); Oxidation (M) | |
| 810.4075 | 1618.8004 | 1618.7739 | 16.4 | 0 | 3 | 0.53 | 1Score > 23 indicates identityScore > 13 indicates homology |
LITAYYNEHGPGQR + Deamidated (NQ) | |
| 922.4689 | 2764.3849 | 2764.3959 | -3.99 | 1 | 3 | 1 | 1Score > 23 indicates identityScore > 16 indicates homology |
QQAAETKELQEIPPQLLFVHWQK + 4 Deamidated (NQ) | |
| 778.8516 | 1555.6886 | 1555.6613 | 17.6 | 0 | 3 | 0.97 | 1Score > 21 indicates identityScore > 16 indicates homology |
EHMSFEDYSGNLK | |
| 729.3427 | 728.3354 | 728.3381 | -3.70 | 0 | 3 | 0.78 | 1Score > 15 indicates identity |
TSLDFF | |
| 434.7182 | 867.4218 | 867.4239 | -2.35 | 0 | 3 | 1 | 1Score > 20 indicates identityScore > 16 indicates homology |
HNSWVPK + Deamidated (NQ) | |
| 530.9440 | 1589.8102 | 1589.7970 | 8.27 | 1 | 3 | 0.65 | 1Score > 22 indicates identityScore > 14 indicates homology |
EAQRIEGMLQLLTS + 2 Deamidated (NQ) | |
| 912.4131 | 1822.8116 | 1822.8155 | -2.13 | 1 | 3 | 2.3 | 1Score > 19 indicates identity |
NNFKIIDSHSMNNSGK + 2 Deamidated (NQ); Oxidation (M) | |
| 1137.5398 | 1136.5325 | 1136.5132 | 17.0 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
AAERDNMLTT + Oxidation (M) | |
| 794.3692 | 793.3619 | 793.3640 | -2.61 | 0 | 3 | 0.66 | 1Score > 21 indicates identityScore > 14 indicates homology |
QLGDSMK + Oxidation (M) | |
| 641.3636 | 640.3563 | 640.3657 | -14.6 | 0 | 3 | 0.97 | 1Score > 16 indicates identity |
VGVNPR | |
| 460.7159 | 919.4172 | 919.4247 | -8.05 | 1 | 3 | 0.92 | 1Score > 21 indicates identityScore > 15 indicates homology |
QNESEGKK + Deamidated (NQ) | |
| 830.8858 | 1659.7570 | 1659.7410 | 9.68 | 1 | 3 | 1 | 1Score > 20 indicates identityScore > 16 indicates homology |
KSHNMPSSILDDGNK + 2 Deamidated (NQ); Oxidation (M) | |
| 570.2716 | 1138.5286 | 1138.5506 | -19.3 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
AGDKYSIEQK + Deamidated (NQ) | |
| 842.9010 | 1683.7874 | 1683.7661 | 12.7 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
QIQEYESMNGNLIK + 2 Deamidated (NQ); Oxidation (M) | |
| 345.1771 | 688.3396 | 688.3293 | 15.1 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
SFNGHK | |
| 848.3794 | 847.3721 | 847.3671 | 5.88 | 0 | 3 | 2.4 | 1Score > 20 indicates identity |
LGNANSDR + 2 Deamidated (NQ) | |
| 623.3515 | 622.3442 | 622.3551 | -17.5 | 1 | 3 | 0.87 | 1Score > 22 indicates identityScore > 15 indicates homology |
HGKGPK | |
| 975.1677 | 2922.4813 | 2922.4572 | 8.24 | 1 | 3 | 1 | 1Score > 20 indicates identityScore > 16 indicates homology |
ISFLQSKLQQFATSSMETYILEQTK + 2 Deamidated (NQ) | |
| 879.8848 | 1757.7550 | 1757.7380 | 9.69 | 0 | 3 | 1.7 | 1Score > 18 indicates identity |
STSPNANANDNTEFFK + 2 Deamidated (NQ) | |
| 625.3329 | 1248.6512 | 1248.6714 | -16.1 | 1 | 3 | 1 | 1Score > 23 indicates identityScore > 16 indicates homology |
LGIKQDNSVFK + Deamidated (NQ) | |
| 487.2389 | 1458.6949 | 1458.7177 | -15.6 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
MTTFRPLSSFEK + Oxidation (M) | |
| 361.7016 | 721.3886 | 721.3911 | -3.46 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
FLTWR | |
| 764.3559 | 1526.6972 | 1526.7221 | -16.3 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
GNSMLFTMNRDIK + Deamidated (NQ) | |
| 667.8414 | 1333.6682 | 1333.6547 | 10.1 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
VENGIMQSISEK | |
| 757.9140 | 1513.8134 | 1513.8075 | 3.94 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
KPTMIKLQFHNR + 2 Deamidated (NQ) | |
| 806.1066 | 2415.2980 | 2415.2686 | 12.2 | 1 | 3 | 1.9 | 1Score > 18 indicates identity |
AIFPNTVDGDLLKLVHLSNGYK + 2 Deamidated (NQ) | |
| 499.2336 | 996.4526 | 996.4400 | 12.7 | 0 | 3 | 0.84 | 1Score > 20 indicates identityScore > 15 indicates homology |
ADSFINNSK + 2 Deamidated (NQ) | |
| 1020.9894 | 2039.9642 | 2039.9946 | -14.9 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
NIGVADPQNSLNPSKPNMK + Deamidated (NQ); Oxidation (M) | |
| 1055.5127 | 1054.5054 | 1054.5083 | -2.77 | 0 | 3 | 1 | 1Score > 20 indicates identityScore > 15 indicates homology |
SINHPPDFK + Deamidated (NQ) | |
| 723.3326 | 722.3253 | 722.3269 | -2.15 | 0 | 3 | 2.3 | 1Score > 19 indicates identity |
MSDNLK + Oxidation (M) | |
| 669.0362 | 2004.0868 | 2004.0568 | 15.0 | 1 | 3 | 1.9 | 1Score > 18 indicates identity |
LPPLRFLDDYIEISADK | |
| 928.4130 | 927.4057 | 927.4158 | -10.9 | 1 | 3 | 1.6 | 1Score > 17 indicates identity |
RTQNDHR + 2 Deamidated (NQ) | |
| 612.3359 | 611.3286 | 611.3391 | -17.1 | 0 | 3 | 0.74 | 1Score > 14 indicates identity |
KPDPR | |
| 618.2739 | 617.2666 | 617.2769 | -16.6 | 0 | 3 | 0.59 | 1Score > 13 indicates identity |
QQGER + Deamidated (NQ) | |
| 858.4194 | 857.4121 | 857.4171 | -5.77 | 0 | 3 | 1.9 | 1Score > 18 indicates identity |
YLSNIFT + Deamidated (NQ) | |
| 814.3699 | 813.3626 | 813.3691 | -7.94 | 0 | 3 | 1.3 | 1Score > 17 indicates identity |
TYVQMR + Deamidated (NQ); Oxidation (M) | |
| 551.2820 | 1100.5494 | 1100.5713 | -19.9 | 1 | 3 | 0.79 | 1Score > 21 indicates identityScore > 14 indicates homology |
TIEPQEEKK | |
| 613.2957 | 612.2884 | 612.2980 | -15.6 | 0 | 3 | 1 | 1Score > 16 indicates identity |
NHSGAK | |
| 586.7639 | 1171.5132 | 1171.5357 | -19.1 | 0 | 3 | 1.2 | 1Score > 16 indicates identity |
IGGVNNPEQNK + 3 Deamidated (NQ) | |
| 759.4263 | 1516.8380 | 1516.8110 | 17.8 | 1 | 3 | 1 | 1Score > 20 indicates identityScore > 15 indicates homology |
LSSASGLNVPGGFRR | |
| 765.9091 | 1529.8036 | 1529.8300 | -17.3 | 1 | 3 | 1 | 1Score > 23 indicates identityScore > 15 indicates homology |
KISQLVQENVTLR + 3 Deamidated (NQ) | |
| 448.2366 | 894.4586 | 894.4672 | -9.52 | 0 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
QPGHLTSR | |
| 762.3441 | 761.3368 | 761.3378 | -1.24 | 0 | 3 | 2.3 | 1Score > 19 indicates identity |
ENSGPMK | |
| 787.3635 | 786.3562 | 786.3508 | 6.94 | 0 | 3 | 2.1 | 1Score > 18 indicates identity |
NDVAPNR + 2 Deamidated (NQ) | |
| 1152.5822 | 2303.1498 | 2303.1501 | -0.096 | 1 | 3 | 0.9 | 1Score > 22 indicates identityScore > 15 indicates homology |
LQQLSESIKITEMQLNHMK + Deamidated (NQ); 2 Oxidation (M) | |
| 940.4294 | 1878.8442 | 1878.8451 | -0.46 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
VRMQGEEQENIMIER + 2 Deamidated (NQ); Oxidation (M) | |
| 997.4606 | 996.4533 | 996.4552 | -1.93 | 0 | 3 | 0.71 | 1Score > 20 indicates identityScore > 14 indicates homology |
VSSWENFK + Deamidated (NQ) | |
| 321.1848 | 640.3550 | 640.3657 | -16.6 | 0 | 3 | 1.1 | 1Score > 16 indicates identity |
VGVNPR | |
| 1024.9808 | 2047.9470 | 2047.9772 | -14.7 | 0 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
PGQIISIPFLSQNEDMDK + Deamidated (NQ); Oxidation (M) | |
| 1134.5542 | 1133.5469 | 1133.5564 | -8.34 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
QLSSKQNAQK + 3 Deamidated (NQ) | |
| 664.8308 | 1327.6470 | 1327.6408 | 4.71 | 0 | 3 | 0.61 | 1Score > 22 indicates identityScore > 13 indicates homology |
NSATILNYFER + Deamidated (NQ) | |
| 1123.5383 | 1122.5310 | 1122.5305 | 0.45 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
SLEDRFNNK + Deamidated (NQ) | |
| 624.3267 | 1246.6388 | 1246.6193 | 15.6 | 0 | 3 | 0.61 | 1Score > 23 indicates identityScore > 13 indicates homology |
AGILNDAWATSK + Deamidated (NQ) | |
| 984.4813 | 1966.9480 | 1966.9346 | 6.84 | 1 | 3 | 1 | 1Score > 23 indicates identityScore > 15 indicates homology |
ANPNPVKFMNTSEIFQK + 3 Deamidated (NQ) | |
| 544.2862 | 1086.5578 | 1086.5709 | -12.0 | 0 | 3 | 0.76 | 1Score > 22 indicates identityScore > 14 indicates homology |
EHTIIAEFK | |
| 656.8348 | 1311.6550 | 1311.6744 | -14.8 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
DVKSQISMIFK + Deamidated (NQ); Oxidation (M) | |
| 801.9228 | 1601.8310 | 1601.8382 | -4.44 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
STVPIIIRCCIDR | |
| 1167.5460 | 3499.6162 | 3499.6518 | -10.2 | 1 | 3 | 1 | 1Score > 20 indicates identityScore > 15 indicates homology |
YPSLLDDSFITLTNREIDDYSDLPSPNPSSK + Deamidated (NQ) | |
| 1096.5283 | 1095.5210 | 1095.5271 | -5.50 | 0 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
ISDFVCLDK | |
| 619.7969 | 1237.5792 | 1237.5575 | 17.6 | 0 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
NPESPESVHNK + Deamidated (NQ) | |
| 639.3300 | 1276.6454 | 1276.6636 | -14.2 | 1 | 3 | 0.84 | 1Score > 23 indicates identityScore > 14 indicates homology |
SPTNNNPRIHK | |
| 490.2278 | 978.4410 | 978.4328 | 8.43 | 0 | 3 | 2.4 | 1Score > 19 indicates identity |
ADSPMQSVK + Deamidated (NQ); Oxidation (M) | |
| 726.3758 | 2176.1056 | 2176.0899 | 7.19 | 1 | 3 | 1 | 1Score > 23 indicates identityScore > 15 indicates homology |
STPFPYLTSSTSSSSLASTKK | |
| 777.3784 | 2329.1134 | 2329.1260 | -5.41 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
MSDNLLSLENPVVPSHYELR + Deamidated (NQ); Oxidation (M) | |
| 880.8611 | 1759.7076 | 1759.7384 | -17.5 | 0 | 3 | 0.88 | 1Score > 15 indicates identity |
TGNEDVGNNNPSNSIPK + 4 Deamidated (NQ) | |
| 1062.5289 | 1061.5216 | 1061.5426 | -19.8 | 0 | 3 | 0.81 | 1Score > 22 indicates identityScore > 14 indicates homology |
LNSELLMNK + Deamidated (NQ) | |
| 583.2970 | 1164.5794 | 1164.5622 | 14.8 | 0 | 3 | 0.84 | 1Score > 23 indicates identityScore > 14 indicates homology |
ITEGSSAASNTK | |
| 718.3619 | 1434.7092 | 1434.7354 | -18.2 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
TSPIVALTAQNYR + 2 Deamidated (NQ) | |
| 750.8644 | 1499.7142 | 1499.7157 | -0.98 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
YHSHNDTIFIPR + Deamidated (NQ) | |
| 315.6645 | 629.3144 | 629.3133 | 1.86 | 0 | 3 | 1.2 | 1Score > 16 indicates identity |
TAQPGR + Deamidated (NQ) | |
| 789.8978 | 1577.7810 | 1577.7533 | 17.6 | 1 | 3 | 1 | 1Score > 23 indicates identityScore > 15 indicates homology |
ERDGSTEETLNSLK | |
| 530.2738 | 1587.7996 | 1587.7715 | 17.7 | 0 | 3 | 0.67 | 1Score > 24 indicates identityScore > 13 indicates homology |
MHDLNIPTFSSIGR + Deamidated (NQ) | |
| 1021.5353 | 1020.5280 | 1020.5200 | 7.89 | 1 | 3 | 0.58 | 1Score > 23 indicates identityScore > 13 indicates homology |
LTSGSGSERK | |
| 766.4274 | 2296.2604 | 2296.2175 | 18.7 | 1 | 3 | 1.6 | 1Score > 17 indicates identity |
IQHHITETILTYASLNKGTR + Deamidated (NQ) | |
| 634.3584 | 633.3511 | 633.3520 | -1.32 | 1 | 3 | 0.95 | 1Score > 23 indicates identityScore > 15 indicates homology |
DKLMK | |
| 516.2711 | 1545.7915 | 1545.8012 | -6.27 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
VASHKNSHNPSLVR + Deamidated (NQ) | |
| 632.9946 | 1895.9620 | 1895.9299 | 16.9 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
DGALLCGILDKSQYGASK + Deamidated (NQ) | |
| 602.8026 | 1203.5906 | 1203.5731 | 14.6 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
GLNVSNNQISR + 3 Deamidated (NQ) | |
| 880.8589 | 1759.7032 | 1759.7384 | -20.0 | 0 | 2 | 0.76 | 1Score > 14 indicates identity |
TGNEDVGNNNPSNSIPK + 4 Deamidated (NQ) | |
| 952.1475 | 2853.4207 | 2853.4648 | -15.4 | 0 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
EYVSLVGQVLLDGSSLSNEEILQFSK | |
| 768.3841 | 2302.1305 | 2302.1297 | 0.34 | 1 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
LIHMERDLSSEVVGQMDAIK + 2 Oxidation (M) | |
| 769.8656 | 1537.7166 | 1537.7233 | -4.32 | 0 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
RPDNQNPEGENLR | |
| 759.8602 | 1517.7058 | 1517.7361 | -20.0 | 1 | 2 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
NLDAEDESLAKWK | |
| 993.4861 | 1984.9576 | 1984.9313 | 13.3 | 1 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
DVQYDAACADLRISFNK | |
| 1151.5692 | 2301.1238 | 2301.1675 | -19.0 | 0 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
LSMESPHVNVFSILTDLDIR + Oxidation (M) | |
| 982.8130 | 2945.4172 | 2945.4440 | -9.11 | 1 | 2 | 0.7 | 1Score > 22 indicates identityScore > 13 indicates homology |
SLGLDVPRPMQVDFSNTLQKNADALGR + 4 Deamidated (NQ) | |
| 468.7134 | 935.4122 | 935.4196 | -7.86 | 0 | 2 | 1 | 1Score > 20 indicates identityScore > 15 indicates homology |
GTAGSGETEK | |
| 872.3660 | 1742.7174 | 1742.7192 | -1.03 | 0 | 2 | 1.4 | 1Score > 16 indicates identity |
EGVLMEDEGNEEYTK + Deamidated (NQ) | |
| 1048.5442 | 1047.5369 | 1047.5309 | 5.77 | 0 | 2 | 1 | 1Score > 23 indicates identityScore > 15 indicates homology |
VGSIGSQSASR | |
| 485.7467 | 969.4788 | 969.4879 | -9.37 | 0 | 2 | 2.7 | 1Score > 19 indicates identity |
APAGGAADAAAK | |
| 1154.5648 | 1153.5575 | 1153.5657 | -7.10 | 1 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
MNMKMLLTR + Deamidated (NQ); Oxidation (M) | |
| 639.3467 | 638.3394 | 638.3428 | -5.29 | 0 | 2 | 1 | 1Score > 20 indicates identityScore > 15 indicates homology |
FTFPK | |
| 406.2207 | 810.4268 | 810.4348 | -9.79 | 0 | 2 | 1 | 1Score > 20 indicates identityScore > 15 indicates homology |
VSPNAAPR | |
| 842.4288 | 1682.8430 | 1682.8726 | -17.6 | 0 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
NAINPLQTNVQILNK + 4 Deamidated (NQ) | |
| 742.8782 | 1483.7418 | 1483.7605 | -12.6 | 1 | 2 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
IDQLNWKMTHAK |
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