MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3738 1021.4788 2040.9430
3739 1021.4890 2040.9634
3740 681.3301 2040.9685
3741 1021.4918 2040.9690
3742 1021.9371 2041.8596
3743 682.9799 2045.9179
3744 1023.9794 2045.9442
3745 1024.4702 2046.9258
3746 1024.4805 2046.9464
3749 683.9824 2048.9254
3751 1026.9712 2051.9278
3752 1026.9838 2051.9530
3753 1028.4830 2054.9514
3754 1028.5042 2054.9938
3755 686.3246 2055.9520
3756 1029.0051 2055.9956
3757 686.3400 2055.9982
3758 686.3409 2056.0009
3759 1029.4778 2056.9410
3760 686.6584 2056.9534
3761 1029.4875 2056.9604
3762 1029.4890 2056.9634
3764 1030.4370 2058.8594
3765 1030.5248 2059.0350
3766 687.3550 2059.0432
3767 1030.5289 2059.0432
3768 687.3554 2059.0444
3769 1030.5366 2059.0586
3770 1030.9282 2059.8418
3771 1031.4807 2060.9468
3772 1031.5022 2060.9898
3773 688.3107 2061.9103
3774 688.6462 2062.9168
3775 1032.4686 2062.9226
3776 1032.4773 2062.9400
3777 1032.4807 2062.9468
3779 1033.4681 2064.9216
3780 689.3153 2064.9241
3781 1033.5007 2064.9868
3782 1033.5042 2064.9938
3783 1033.5192 2065.0238
3784 1033.5209 2065.0272
3785 1033.5355 2065.0564
3786 689.6736 2065.9990
3787 1034.5144 2067.0142
3789 1035.5118 2069.0090
3790 1036.4983 2070.9820
3791 1036.5005 2070.9864
3792 691.6694 2071.9864
3793 1037.4943 2072.9740
3794 1037.5032 2072.9918
3795 1037.5464 2073.0782
3796 1037.5529 2073.0912
3797 692.3404 2073.9994
3798 1038.0470 2074.0794
3799 1038.0530 2074.0914
3800 1038.0551 2074.0956
3801 1038.0552 2074.0958
3802 1038.0563 2074.0980
3803 692.3739 2074.0999
3804 692.3762 2074.1068
3805 692.7200 2075.1382
3806 692.7208 2075.1406
3807 1038.9883 2075.9620
3808 1039.4819 2076.9492
3809 693.3287 2076.9643
3810 1039.4935 2076.9724
3811 1039.4968 2076.9790
3812 1039.4993 2076.9840
3813 693.3371 2076.9895
3814 693.6324 2077.8754
3815 1039.9626 2077.9106
3816 693.6554 2077.9444
3817 693.9776 2078.9110
3818 1040.4738 2078.9330
3819 1040.4756 2078.9366
3820 694.3193 2079.9361
3821 1041.5015 2080.9884
3822 1041.5089 2081.0032
3823 1042.5002 2082.9858
3824 1042.5087 2083.0028
3825 1042.5200 2083.0254
3826 695.6714 2083.9924
3827 1043.4850 2084.9554
3828 695.9944 2084.9614
3829 1043.4956 2084.9766
3830 1043.4988 2084.9830
3831 1043.5002 2084.9858
3832 696.3525 2086.0357
3833 1044.0919 2086.1692
3834 696.3976 2086.1710
3835 1044.0930 2086.1714
3836 696.4003 2086.1791
3837 1044.4862 2086.9578
3838 1044.4990 2086.9834
3839 696.6867 2087.0383
3840 697.3273 2088.9601
3841 1045.4987 2088.9828
3842 1045.5160 2089.0174
3843 1045.8134 2089.6122
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