MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3625 977.9836 1953.9526
3626 978.4681 1954.9216
3627 982.4749 1962.9352
3628 982.4812 1962.9478
3629 983.4479 1964.8812
3630 983.4482 1964.8818
3632 656.0320 1965.0742
3634 984.4781 1966.9416
3635 984.4796 1966.9446
3637 656.6620 1966.9642
3638 656.9893 1967.9461
3639 984.9811 1967.9476
3640 984.9880 1967.9614
3641 985.4874 1968.9602
3642 985.4894 1968.9642
3643 657.3294 1968.9664
3644 985.4927 1968.9708
3645 987.0695 1972.1244
3646 658.3857 1972.1353
3647 987.4800 1972.9454
3648 987.9852 1973.9558
3649 659.3157 1974.9253
3650 494.9861 1975.9153
3651 990.4655 1978.9164
3652 990.4800 1978.9454
3653 991.4465 1980.8784
3654 991.4885 1980.9624
3657 661.6443 1981.9111
3658 992.0036 1981.9926
3659 992.4778 1982.9410
3660 662.3203 1983.9391
3661 992.9774 1983.9402
3662 992.9802 1983.9458
3665 663.3239 1986.9499
3666 995.4496 1988.8846
3667 498.2387 1988.9257
3668 995.4705 1988.9264
3669 995.4843 1988.9540
3671 995.9827 1989.9508
3673 996.4654 1990.9162
3674 996.4917 1990.9688
3675 665.3267 1992.9583
3676 997.4998 1992.9850
3677 997.9599 1993.9052
3678 665.6491 1993.9255
3681 666.0026 1994.9860
3682 998.5026 1994.9906
3683 998.5045 1994.9944
3684 999.4908 1996.9670
3685 1000.4694 1998.9242
3686 1000.4761 1998.9376
3687 1000.4812 1998.9478
3688 1000.4996 1998.9846
3689 1000.9757 1999.9368
3691 1003.4502 2004.8858
3692 669.3066 2004.8980
3693 1003.4639 2004.9132
3694 1003.4689 2004.9232
3695 669.3408 2005.0006
3696 1004.4576 2006.9006
3697 670.0004 2006.9794
3698 670.3311 2007.9715
3699 670.3350 2007.9832
3700 1005.4940 2008.9734
3701 1005.4957 2008.9768
3702 1005.5093 2009.0040
3703 670.6767 2009.0083
3704 1005.5118 2009.0090
3705 1005.9927 2009.9708
3706 1006.0061 2009.9976
3707 1007.9920 2013.9694
3708 1008.5046 2014.9946
3709 1009.4633 2016.9120
3710 1010.7199 2019.4252
3711 1011.4466 2020.8786
3712 674.6358 2020.8856
3713 1011.4581 2020.9016
3714 674.6495 2020.9267
3715 1011.4714 2020.9282
3716 1011.4755 2020.9364
3717 1011.4794 2020.9442
3718 675.6650 2023.9732
3719 1013.4766 2024.9386
3720 1013.4915 2024.9684
3721 675.9975 2024.9707
3722 1013.4941 2024.9736
3723 1013.5035 2024.9924
3724 1014.0004 2025.9862
3725 1014.5019 2026.9892
3726 1016.4883 2030.9620
3727 1017.0280 2032.0414
3728 679.3189 2034.9349
3729 1018.4789 2034.9432
3730 1020.4946 2038.9746
3731 680.6791 2039.0155
3732 1020.5200 2039.0254
3733 1020.9557 2039.8968
3734 680.9948 2039.9626
3736 1021.0137 2040.0128
3737 1021.4461 2040.8776
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