MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3514 927.4623 1852.9100
3515 927.4695 1852.9244
3516 929.4509 1856.8872
3517 620.5789 1858.7149
3518 930.4396 1858.8646
3519 930.4410 1858.8674
3520 930.4557 1858.8968
3521 621.2968 1860.8686
3522 931.4420 1860.8694
3523 932.4334 1862.8522
3524 932.9418 1863.8690
3525 933.4346 1864.8546
3526 933.4442 1864.8738
3527 933.4504 1864.8862
3528 934.0507 1866.0868
3529 623.0374 1866.0904
3530 934.5547 1867.0948
3531 623.3723 1867.0951
3532 935.9468 1869.8790
3533 935.9537 1869.8928
3534 624.9827 1871.9263
3535 937.4395 1872.8644
3537 938.4778 1874.9410
3538 626.3016 1875.8830
3539 938.9766 1875.9386
3540 939.4430 1876.8714
3541 940.4243 1878.8340
3544 630.3141 1887.9205
3545 630.3212 1887.9418
3546 945.4317 1888.8488
3547 945.4468 1888.8790
3548 630.6346 1888.8820
3549 945.4553 1888.8960
3550 945.9370 1889.8594
3551 946.4565 1890.8984
3552 631.6307 1891.8703
3553 947.4399 1892.8652
3555 947.5022 1892.9898
3556 948.4896 1894.9646
3557 948.4908 1894.9670
3559 949.9708 1897.9270
3560 951.4466 1900.8786
3561 952.4507 1902.8868
3562 952.9564 1903.8982
3563 953.4343 1904.8540
3564 953.4425 1904.8704
3565 953.9420 1905.8694
3566 636.2997 1905.8773 1905.8738 1.84 1     +0Score > 21 indicates identity
Score > 13 indicates homology		 MSENNGNPLDLSSLRNK + 2 Deamidated (NQ); Oxidation (M)
3568 956.4781 1910.9416
3569 637.9906 1910.9500
3570 956.9580 1911.9014
3571 638.3222 1911.9448
3572 956.9797 1911.9448
3573 956.9904 1911.9662
3574 957.4598 1912.9050
3575 957.4603 1912.9060
3576 957.4633 1912.9120
3577 960.4457 1918.8768
3579 961.4498 1920.8850
3580 961.9333 1921.8520
3581 641.6675 1921.9807
3582 962.0008 1921.9870
3583 962.4522 1922.8898
3584 963.4432 1924.8718
3585 964.4469 1926.8792
3586 964.4734 1926.9322
3587 964.4764 1926.9382
3588 965.4461 1928.8776
3589 965.4586 1928.9026
3590 643.9906 1928.9500
3591 966.4553 1930.8960
3592 967.4548 1932.8950
3593 967.4620 1932.9094
3596 968.5120 1935.0094
3597 646.3397 1935.9973
3598 969.4893 1936.9640
3599 969.5031 1936.9916
3600 646.9969 1937.9689
3601 969.9921 1937.9696
3602 646.9978 1937.9716
3603 970.4443 1938.8740
3604 971.4497 1940.8848
3605 971.4789 1940.9432
3607 972.9709 1943.9272
3608 649.3209 1944.9409
3609 488.2301 1948.8913
3610 975.4532 1948.8918
3611 488.4798 1949.8901
3612 651.3002 1950.8788
3613 976.4490 1950.8834
3614 976.4493 1950.8840
3616 976.4865 1950.9584
3617 976.5102 1951.0058
3618 976.9827 1951.9508
3619 651.6590 1951.9552
3620 976.9997 1951.9848
3621 651.6690 1951.9852
3622 651.6699 1951.9879
3623 977.4792 1952.9438
3624 977.4959 1952.9772
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