MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3288 557.3365 1668.9877
3289 836.9356 1671.8566
3290 836.9390 1671.8634
3291 558.2967 1671.8683
3292 836.9420 1671.8694
3293 838.3986 1674.7826
3294 838.8848 1675.7550
3295 839.3890 1676.7634
3296 560.9274 1679.7604
3297 840.8875 1679.7604
3298 841.3867 1680.7588
3299 561.2606 1680.7600
3303 842.9026 1683.7906
3304 843.3928 1684.7710
3305 843.3931 1684.7716
3306 843.4047 1684.7948
3307 843.4103 1684.8060
3308 563.6136 1687.8190
3309 844.9175 1687.8204
3310 845.4130 1688.8114
3311 845.4279 1688.8412
3312 845.9091 1689.8036
3314 564.6327 1690.8763
3315 846.4486 1690.8826
3316 846.9482 1691.8818
3317 847.3821 1692.7496
3319 847.3845 1692.7544
3320 847.4340 1692.8534
3321 848.4208 1694.8270
3322 566.2579 1695.7519
3323 849.4070 1696.7994
3324 849.9186 1697.8226
3325 850.4200 1698.8254
3326 851.3883 1700.7620
3327 568.6175 1702.8307
3329 853.4165 1704.8184
3330 853.4182 1704.8218
3331 853.4362 1704.8578
3332 853.8923 1705.7700
3333 853.9214 1705.8282
3334 854.0135 1706.0124
3335 854.8796 1707.7446
3336 854.8827 1707.7508
3337 854.8930 1707.7714
3338 854.9155 1707.8164
3339 855.3578 1708.7010
3340 855.3674 1708.7202
3341 855.4078 1708.8010
3342 855.8743 1709.7340
3343 856.4030 1710.7914
3345 571.3051 1710.8935
3347 572.2412 1713.7018
3348 858.4210 1714.8274
3349 858.4417 1714.8688
3350 859.9435 1717.8724
3351 573.9492 1718.8258
3352 861.4089 1720.8032
3353 575.2481 1722.7225
3354 862.3760 1722.7374
3355 575.2546 1722.7420
3356 862.4059 1722.7972
3357 863.4095 1724.8044
3358 863.4122 1724.8098
3359 863.4278 1724.8410
3360 576.2763 1725.8071
3362 864.4295 1726.8444
3363 864.4312 1726.8478
3364 576.6308 1726.8706
3365 866.9254 1731.8362
3366 867.3951 1732.7756
3367 868.3930 1734.7714
3368 868.4095 1734.8044
3369 868.4207 1734.8268
3370 869.3979 1736.7812
3371 579.9346 1736.7820
3372 871.4116 1740.8086
3373 871.8676 1741.7206
3374 871.9055 1741.7964
3376 872.4241 1742.8336
3377 872.8564 1743.6982
3378 582.2406 1743.7000
3379 873.4358 1744.8570
3380 873.9319 1745.8492
3381 874.4316 1746.8486
3382 583.3054 1746.8944
3383 583.6411 1747.9015
3384 875.3929 1748.7712
3385 875.4221 1748.8296
3386 875.8920 1749.7694
3387 875.8985 1749.7824
3388 584.2691 1749.7855
3389 875.9395 1749.8644
3390 876.3940 1750.7734
3391 876.4332 1750.8518
3393 878.4264 1754.8382
3394 585.9856 1754.9350
3396 879.8645 1757.7144
3398 587.2449 1758.7129
3399 880.4234 1758.8322
3400 880.4650 1758.9154
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