MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3032 754.3163 1506.6180
3033 754.3590 1506.7034
3034 754.3984 1506.7822
3035 754.3987 1506.7828
3036 754.8758 1507.7370
3037 755.3267 1508.6388
3038 755.3407 1508.6668
3039 755.8319 1509.6492
3040 504.2250 1509.6532
3041 504.2528 1509.7366
3042 756.8368 1511.6590
3043 756.8787 1511.7428
3044 756.8789 1511.7432
3045 756.8804 1511.7462
3046 757.1274 1512.2402
3047 757.3724 1512.7302
3048 757.3735 1512.7324
3049 505.5619 1513.6639
3050 379.4237 1513.6657
3053 758.8687 1515.7228
3054 506.2484 1515.7234
3055 759.3870 1516.7594
3058 506.9098 1517.7076
3059 506.9099 1517.7079
3060 759.8675 1517.7204
3062 760.8637 1519.7128
3063 761.3381 1520.6616
3064 761.3741 1520.7336
3065 761.8459 1521.6772
3066 762.3615 1522.7084
3067 762.3938 1522.7730
3068 762.9316 1523.8486
3069 509.2592 1524.7558
3070 509.5849 1525.7329
3072 510.2246 1527.6520
3073 382.9206 1527.6533
3077 765.9110 1529.8074
3078 766.8549 1531.6952
3079 767.3678 1532.7210
3080 767.3732 1532.7318
3082 767.3861 1532.7576
3083 767.4020 1532.7894
3084 767.4033 1532.7920
3085 767.8566 1533.6986
3086 512.2408 1533.7006
3087 768.3569 1534.6992
3088 768.3684 1534.7222
3089 768.3777 1534.7408
3090 768.4009 1534.7872
3091 512.9005 1535.6797
3092 768.8519 1535.6892
3094 769.3375 1536.6604
3096 513.5787 1537.7143
3098 769.8875 1537.7604
3099 770.3600 1538.7054
3101 514.5887 1540.7443
3102 515.2134 1542.6184
3103 773.8156 1545.6166
3108 774.3712 1546.7278
3109 774.3973 1546.7800
3110 774.8619 1547.7092
3111 775.3521 1548.6896
3112 775.3663 1548.7180
3114 775.8534 1549.6922
3115 775.8615 1549.7084
3116 776.4322 1550.8498
3118 776.8959 1551.7772
3119 777.8825 1553.7504
3120 778.3500 1554.6854
3121 778.3782 1554.7418
3124 779.4203 1556.8260
3125 780.3824 1558.7502
3126 780.3836 1558.7526
3127 781.3878 1560.7610
3128 781.8334 1561.6522
3130 781.8953 1561.7760
3131 781.8963 1561.7780
3135 781.9230 1561.8314
3137 521.9329 1562.7769
3139 782.8590 1563.7034
3141 785.8810 1569.7474
3142 786.3863 1570.7580
3143 786.4323 1570.8500
3144 786.9142 1571.8138
3145 786.9166 1571.8186
3146 787.3915 1572.7684
3147 787.8942 1573.7738
3148 787.9156 1573.8166
3149 787.9174 1573.8202
3150 525.9161 1574.7265
3151 788.3912 1574.7678
3152 789.3926 1576.7706
3154 789.8980 1577.7814
3158 790.8860 1579.7574
3159 791.3923 1580.7700
3160 528.2373 1581.6901
3161 792.8664 1583.7182
3162 793.3878 1584.7610
3163 397.4418 1585.7381
3164 529.5869 1585.7389
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