MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2898 710.8590 1419.7034
2899 711.3394 1420.6642
2900 712.3275 1422.6404
2901 713.3555 1424.6964
2902 713.8575 1425.7004
2905 714.3232 1426.6318
2906 715.8342 1429.6538
2907 715.8344 1429.6542
2908 715.8688 1429.7230
2909 715.8711 1429.7276
2910 716.3858 1430.7570
2911 716.8156 1431.6166
2912 716.8862 1431.7578
2913 717.3465 1432.6784
2915 717.8914 1433.7682
2916 717.8915 1433.7684
2919 719.3361 1436.6576
2920 719.8282 1437.6418
2921 720.8623 1439.7100
2922 720.8993 1439.7840
2923 720.9015 1439.7884
2924 721.3621 1440.7096
2925 721.3626 1440.7106
2926 722.3556 1442.6966
2927 722.8797 1443.7448
2928 723.8537 1445.6928
2932 482.9133 1445.7181
2934 723.8687 1445.7228
2936 723.8718 1445.7290
2937 723.8724 1445.7302
2938 723.8734 1445.7322
2940 723.8737 1445.7328
2944 723.8757 1445.7368
2945 723.8767 1445.7388
2946 723.8768 1445.7390
2947 723.8769 1445.7392
2948 723.8770 1445.7394
2949 723.8771 1445.7396
2950 723.8773 1445.7400
2951 724.3547 1446.6948
2952 724.3734 1446.7322
2953 724.3785 1446.7424
2957 726.3649 1450.7152
2958 726.3707 1450.7268
2959 726.3734 1450.7322
2960 726.3760 1450.7374
2961 728.3549 1454.6952
2962 728.3568 1454.6990
2963 728.8622 1455.7098
2964 728.8878 1455.7610
2966 486.8900 1457.6482
2969 730.3875 1458.7604
2970 730.8735 1459.7324
2971 730.8738 1459.7330
2973 730.8983 1459.7820
2975 730.8994 1459.7842
2976 731.8542 1461.6938
2977 731.8552 1461.6958
2978 731.8631 1461.7116
2979 732.3683 1462.7220
2980 733.3468 1464.6790
2983 735.8694 1469.7242
2984 736.3522 1470.6898
2986 492.2734 1473.7984
2987 737.9067 1473.7988
2988 737.9069 1473.7992
2989 739.3683 1476.7220
2990 739.8504 1477.6862
2991 739.8611 1477.7076
2992 739.8632 1477.7118
2993 741.3475 1480.6804
2994 741.3798 1480.7450
2995 742.3394 1482.6642
2996 742.3757 1482.7368
2997 742.3830 1482.7514
2998 495.5620 1483.6642
2999 495.5801 1483.7185
3000 495.5805 1483.7197
3003 743.3692 1484.7238
3004 743.3740 1484.7334
3005 743.8775 1485.7404
3006 745.3616 1488.7086
3007 745.8514 1489.6882
3010 746.8563 1491.6980
3011 746.8653 1491.7160
3012 746.8655 1491.7164
3013 748.3442 1494.6738
3014 748.3577 1494.7008
3016 748.8489 1495.6832
3017 749.3491 1496.6836
3019 749.3754 1496.7362
3020 750.1289 1498.2432
3021 500.9113 1499.7121
3023 500.9124 1499.7154
3024 751.8702 1501.7258
3025 751.8715 1501.7284
3027 752.8786 1503.7426
3029 752.8980 1503.7814
3030 753.3605 1504.7064
3031 753.8569 1505.6992
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