MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2749 633.3350 1264.6554
2750 633.3370 1264.6594
2754 635.8292 1269.6438
2755 635.8306 1269.6466
2757 636.2981 1270.5816
2759 636.3041 1270.5936
2760 636.3086 1270.6026
2761 636.3211 1270.6276
2762 637.3759 1272.7372
2763 638.3208 1274.6270
2764 638.7938 1275.5730
2765 638.8336 1275.6526
2767 638.8379 1275.6612
2770 640.2960 1278.5774
2771 640.2975 1278.5804
2772 640.8094 1279.6042
2774 643.3344 1284.6542
2776 644.2968 1286.5790
2777 644.2999 1286.5852
2778 322.6560 1286.5949
2779 429.8734 1286.5984
2780 431.5590 1291.6552
2781 647.3273 1292.6400
2783 649.8223 1297.6300
2784 649.8245 1297.6344
2785 649.8259 1297.6372
2787 651.3263 1300.6380
2788 651.3858 1300.7570
2789 651.8304 1301.6462
2791 652.3391 1302.6636
2794 655.3245 1308.6344
2799 438.8737 1313.5993
2805 660.8267 1319.6388
2806 661.8159 1321.6172
2807 662.8403 1323.6660
2808 662.8406 1323.6666
2809 663.3093 1324.6040
2810 663.3507 1324.6868
2811 663.7976 1325.5806
2812 664.8091 1327.6036
2815 665.3347 1328.6548
2816 666.8326 1331.6506
2818 667.3431 1332.6716
2819 667.3451 1332.6756
2820 667.3463 1332.6780
2822 667.8426 1333.6706
2824 669.3468 1336.6790
2825 669.8578 1337.7010
2826 669.8591 1337.7036
2827 670.2890 1338.5634
2830 674.3290 1346.6434
2832 674.3544 1346.6942
2833 674.8064 1347.5982
2834 450.2074 1347.6004
2835 674.8106 1347.6066
2836 674.8301 1347.6456
2837 450.2409 1347.7009
2838 676.3840 1350.7534
2842 677.3448 1352.6750
2845 454.2294 1359.6664
2846 682.3317 1362.6488
2847 682.3728 1362.7310
2848 682.3735 1362.7324
2849 682.8069 1363.5992
2850 682.9033 1363.7920
2851 683.2986 1364.5826
2852 683.8351 1365.6556
2853 456.5819 1366.7239
2855 685.3445 1368.6744
2856 686.8875 1371.7604
2858 688.8447 1375.6748
2859 688.8450 1375.6754
2861 690.3277 1378.6408
2862 690.3521 1378.6896
2863 691.3232 1380.6318
2864 691.8123 1381.6100
2865 692.8527 1383.6908
2866 462.5396 1384.5970 1384.5929 2.98 1     +0Score > 18 indicates identity YNNENGSMPSKK + Deamidated (NQ); Oxidation (M)
2868 693.3460 1384.6774
2869 462.5688 1384.6846
2871 695.3452 1388.6758
2874 695.8356 1389.6566
2877 464.5463 1390.6171
2878 696.3461 1390.6776
2879 696.8284 1391.6422
2880 697.8577 1393.7008
2881 699.8775 1397.7404
2882 699.9013 1397.7880
2883 700.3735 1398.7324
2884 700.3747 1398.7348
2885 701.3397 1400.6648
2886 701.3862 1400.7578
2887 701.7867 1401.5588
2888 702.8604 1403.7062
2889 702.8622 1403.7098
2890 703.8478 1405.6810
2891 703.9268 1405.8390
2895 710.3214 1418.6282
2896 710.8577 1419.7008
2897 710.8588 1419.7030
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