MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2497 580.7657 1159.5168
2498 1160.5427 1159.5354
2499 1160.5441 1159.5368
2500 1160.5516 1159.5443
2501 1160.5609 1159.5536
2502 580.7850 1159.5554
2504 1160.5640 1159.5567
2505 1161.5416 1160.5343
2506 1161.5428 1160.5355
2507 1161.5588 1160.5515
2508 1162.5522 1161.5449
2509 1162.5641 1161.5568
2511 581.8035 1161.5924
2512 581.8158 1161.6170
2513 581.8159 1161.6172
2514 1163.5446 1162.5373
2515 1163.5562 1162.5489
2516 1163.5612 1162.5539
2517 582.2987 1162.5828
2518 1164.5415 1163.5342
2519 1164.5483 1163.5410
2520 1164.5507 1163.5434
2521 1164.5581 1163.5508
2522 1164.5613 1163.5540
2523 1164.5630 1163.5557
2524 582.8063 1163.5980
2526 1164.6144 1163.6071
2528 1165.5347 1164.5274
2529 1165.5403 1164.5330
2531 1165.5604 1164.5531
2534 1165.5641 1164.5568
2536 583.7777 1165.5408
2538 1166.5737 1165.5664
2539 1167.5378 1166.5305
2540 1167.5638 1166.5565
2541 584.2955 1166.5764
2542 584.3101 1166.6056
2544 584.7571 1167.4996
2545 1168.5419 1167.5346
2546 1169.5582 1168.5509
2547 1169.5731 1168.5658
2548 1170.5350 1169.5277
2549 1170.5458 1169.5385
2550 585.7809 1169.5472
2551 585.7824 1169.5502
2552 1170.5654 1169.5581
2553 1170.5741 1169.5668
2555 1171.5435 1170.5362
2556 1171.5559 1170.5486
2557 1171.5559 1170.5486
2558 1171.5566 1170.5493
2559 1171.5598 1170.5525
2561 1172.5585 1171.5512
2562 1172.5594 1171.5521
2563 1173.5388 1172.5315
2564 1173.5513 1172.5440
2565 1173.5531 1172.5458
2566 1173.5535 1172.5462
2567 1173.5554 1172.5481
2568 1174.5524 1173.5451
2569 1174.5626 1173.5553
2570 1175.5500 1174.5427
2571 1175.5642 1174.5569
2572 1176.5431 1175.5358
2573 1176.5535 1175.5462
2574 588.7809 1175.5472
2575 1176.5549 1175.5476
2576 588.7815 1175.5484
2577 1176.5657 1175.5584
2578 588.8058 1175.5970
2579 588.8234 1175.6322
2580 1177.5575 1176.5502
2581 1177.5665 1176.5592
2583 589.2988 1176.5830
2584 589.3006 1176.5866
2586 1178.5494 1177.5421
2587 1178.5529 1177.5456
2588 1178.5574 1177.5501
2589 1178.5677 1177.5604
2591 589.7945 1177.5744
2592 589.8135 1177.6124
2595 589.8694 1177.7242
2596 1179.5492 1178.5419
2599 1179.5713 1178.5640
2600 590.2895 1178.5644
2601 1179.5759 1178.5686
2602 591.2763 1180.5380
2603 1181.5470 1180.5397
2604 591.2800 1180.5454
2605 1181.5670 1180.5597
2606 1182.5651 1181.5578
2607 1182.5673 1181.5600
2609 591.8110 1181.6074
2610 591.8115 1181.6084
2611 591.8121 1181.6096
2612 592.2719 1182.5292
2614 1183.5615 1182.5542
2615 592.2950 1182.5754
2616 1183.7129 1182.7056
2623 1184.5549 1183.5476
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