MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2377 1133.5421 1132.5348
2378 1133.5435 1132.5362
2379 567.2842 1132.5538
2381 1133.5635 1132.5562
2383 1134.0422 1133.0349
2384 1134.5372 1133.5299
2387 1135.5358 1134.5285
2388 1135.5460 1134.5387
2389 1136.5288 1135.5215
2390 1136.5421 1135.5348
2392 1137.5442 1136.5369
2393 1137.5481 1136.5408
2394 1137.5492 1136.5419
2395 1138.5327 1137.5254
2396 1138.5447 1137.5374
2397 1138.5542 1137.5469
2399 1139.5394 1138.5321
2400 1139.5428 1138.5355
2401 1139.5587 1138.5514
2403 1140.5461 1139.5388
2404 1140.5559 1139.5486
2405 1141.5419 1140.5346
2406 1141.5444 1140.5371
2407 1141.5468 1140.5395
2408 1141.5526 1140.5453
2409 571.3069 1140.5992
2411 1142.5145 1141.5072
2413 1142.5173 1141.5100
2415 1142.5392 1141.5319
2416 1142.5493 1141.5420
2417 571.7803 1141.5460
2418 1143.5359 1142.5286
2419 1143.5426 1142.5353
2420 1143.5497 1142.5424
2421 1143.5511 1142.5438
2422 1143.5520 1142.5447
2423 1144.5432 1143.5359
2424 1144.5551 1143.5478
2425 1145.5282 1144.5209
2426 1145.5504 1144.5431
2427 1145.5513 1144.5440
2430 1145.6328 1144.6255
2431 573.3209 1144.6272
2433 1145.6473 1144.6400
2434 1146.5250 1145.5177
2435 1146.5397 1145.5324
2436 1146.5409 1145.5336
2437 1146.5487 1145.5414
2438 1146.5496 1145.5423
2439 1146.5538 1145.5465
2440 573.8188 1145.6230
2441 1147.5410 1146.5337
2442 1148.5326 1147.5253
2443 1148.5465 1147.5392
2444 1148.5493 1147.5420
2445 1148.5527 1147.5454
2446 1149.3650 1148.3577
2447 1149.5361 1148.5288
2448 1149.5375 1148.5302
2449 1149.5377 1148.5304
2450 575.2814 1148.5482
2451 1149.5569 1148.5496
2452 575.2866 1148.5586
2455 1150.5360 1149.5287
2456 575.7753 1149.5360
2457 1150.5453 1149.5380
2458 1150.5455 1149.5382
2459 1150.5493 1149.5420
2460 575.7801 1149.5456
2461 1151.5360 1150.5287
2462 1151.5510 1150.5437
2463 1151.5531 1150.5458
2465 576.7687 1151.5228
2467 1152.5444 1151.5371
2468 1152.5687 1151.5614
2469 577.2596 1152.5046
2470 1153.5459 1152.5386
2471 1153.5497 1152.5424
2472 1153.5557 1152.5484
2473 1153.5580 1152.5507
2474 1154.5376 1153.5303
2475 577.7846 1153.5546
2477 577.7866 1153.5586
2478 385.5409 1153.6009
2481 1155.5427 1154.5354
2482 1155.5471 1154.5398
2483 1155.5508 1154.5435
2484 1155.5531 1154.5458
2485 578.7677 1155.5208
2486 1156.5409 1155.5336
2487 1156.5457 1155.5384
2488 1157.5443 1156.5370
2489 1157.5538 1156.5465
2490 579.7592 1157.5038
2491 1158.5115 1157.5042
2492 1158.5350 1157.5277
2493 1158.5503 1157.5430
2494 1158.5568 1157.5495
2495 1159.5551 1158.5478
2496 1160.5210 1159.5137
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