MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2263 1102.5186 1101.5113
2264 1102.5204 1101.5131
2265 1102.5323 1101.5250
2268 1103.5245 1102.5172
2269 1103.5250 1102.5177
2270 1103.5277 1102.5204
2271 1103.5292 1102.5219
2273 1104.5164 1103.5091
2274 1104.5184 1103.5111
2276 1105.5232 1104.5159
2277 1105.5272 1104.5199
2278 553.2883 1104.5620
2282 1106.5063 1105.4990
2283 1106.5166 1105.5093
2284 1106.5304 1105.5231
2285 1106.5309 1105.5236
2286 1106.5331 1105.5258
2287 1107.5146 1106.5073
2288 1107.5282 1106.5209
2289 1108.5161 1107.5088
2290 1108.5277 1107.5204
2291 1108.5383 1107.5310
2292 1108.5656 1107.5583
2293 1109.5200 1108.5127
2294 1109.5237 1108.5164
2295 1109.5415 1108.5342
2296 1110.5334 1109.5261
2297 1110.5347 1109.5274
2298 1110.5503 1109.5430
2299 1111.4952 1110.4879
2300 1111.5289 1110.5216
2301 1111.5302 1110.5229
2302 1111.5354 1110.5281
2303 1111.5356 1110.5283
2304 1112.5292 1111.5219
2305 1112.5403 1111.5330
2306 1113.5262 1112.5189
2307 1113.5359 1112.5286
2308 1113.5409 1112.5336
2309 557.7590 1113.5034
2310 557.7614 1113.5082
2312 1115.5162 1114.5089
2313 1115.5269 1114.5196
2314 1115.5452 1114.5379
2315 1116.5022 1115.4949
2316 1116.5260 1115.5187
2317 1116.5378 1115.5305
2318 1117.5348 1116.5275
2319 559.2886 1116.5626
2320 1118.5264 1117.5191
2321 1118.5295 1117.5222
2322 1118.5302 1117.5229
2323 1118.5348 1117.5275
2324 1118.5363 1117.5290
2325 1118.5422 1117.5349
2327 1119.5060 1118.4987
2328 1119.5319 1118.5246
2329 1119.5404 1118.5331
2330 1120.5271 1119.5198
2331 1120.5287 1119.5214
2332 1120.5309 1119.5236
2334 1120.5388 1119.5315
2335 560.7786 1119.5426
2336 1121.5229 1120.5156
2337 1121.5306 1120.5233
2338 1122.5004 1121.4931
2339 561.7574 1121.5002
2341 1122.5293 1121.5220
2342 1122.5332 1121.5259
2343 1122.5422 1121.5349
2344 1122.5486 1121.5413
2346 1123.5393 1122.5320
2347 1124.5216 1123.5143
2348 1124.5287 1123.5214
2350 1125.5259 1124.5186
2351 1125.5303 1124.5230
2352 1125.5305 1124.5232
2353 563.2690 1124.5234
2354 1125.5420 1124.5347
2355 1125.5421 1124.5348
2356 1125.5431 1124.5358
2357 563.7603 1125.5060
2359 563.7623 1125.5100
2360 1127.5309 1126.5236
2361 1127.5424 1126.5351
2362 1127.5443 1126.5370
2363 1128.5345 1127.5272
2364 1128.5422 1127.5349
2365 1129.5286 1128.5213
2366 1129.5334 1128.5261
2367 1129.5454 1128.5381
2368 1130.5289 1129.5216
2369 1131.5312 1130.5239
2370 1131.5361 1130.5288
2371 1132.5403 1131.5330
2372 1132.5427 1131.5354
2373 1132.5433 1131.5360
2374 1132.5452 1131.5379
2375 1133.5347 1132.5274
2376 1133.5366 1132.5293
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