MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4764)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2141 1076.5073 1075.5000
2142 1076.5116 1075.5043
2144 1076.5233 1075.5160
2145 1076.5363 1075.5290
2146 1077.1761 1076.1688
2147 1077.5251 1076.5178
2148 1077.5298 1076.5225
2149 1077.5320 1076.5247
2150 1078.5100 1077.5027
2151 1078.5160 1077.5087
2152 1079.5024 1078.4951
2155 1079.5126 1078.5053
2156 1079.5177 1078.5104
2157 1079.5295 1078.5222
2158 1079.5310 1078.5237
2159 540.2770 1078.5394
2160 540.2816 1078.5486
2161 1080.5056 1079.4983
2162 1080.5107 1079.5034
2164 540.7950 1079.5754
2165 1081.4929 1080.4856
2166 1081.5140 1080.5067
2167 1081.5157 1080.5084
2168 1081.5167 1080.5094
2169 1081.5187 1080.5114
2170 541.2631 1080.5116
2172 1081.5280 1080.5207
2173 1082.4895 1081.4822
2174 1082.5087 1081.5014
2175 1082.5135 1081.5062
2176 1082.5269 1081.5196
2177 1083.5282 1082.5209
2178 1084.5096 1083.5023
2179 1084.5106 1083.5033
2181 1084.5154 1083.5081
2182 1084.5167 1083.5094
2183 1084.5291 1083.5218
2184 542.7682 1083.5218
2185 1084.5292 1083.5219
2188 1085.5164 1084.5091
2189 1085.5239 1084.5166
2190 1085.5254 1084.5181
2191 1086.5027 1085.4954
2192 1087.5131 1086.5058
2195 1088.5054 1087.4981
2196 1088.5101 1087.5028
2197 1088.5239 1087.5166
2198 1088.5292 1087.5219
2203 1089.5027 1088.4954
2204 1089.5138 1088.5065
2205 1089.5238 1088.5165
2206 545.2943 1088.5740
2207 1090.5057 1089.4984
2208 1090.5236 1089.5163
2209 1090.5253 1089.5180
2210 1091.5134 1090.5061
2211 1091.5269 1090.5196
2212 1091.5288 1090.5215
2213 1091.5332 1090.5259
2214 1091.5358 1090.5285
2215 1091.5450 1090.5377
2216 1092.4952 1091.4879
2217 1092.5234 1091.5161
2218 546.7710 1091.5274
2219 1092.5398 1091.5325
2220 1093.5021 1092.4948
2222 1093.5227 1092.5154
2223 1093.5258 1092.5185
2224 1093.5260 1092.5187
2225 1093.5278 1092.5205
2226 1093.5327 1092.5254
2227 1093.5385 1092.5312
2228 1094.5162 1093.5089
2229 1094.5178 1093.5105
2230 547.7661 1093.5176
2231 1094.5251 1093.5178
2234 1095.5168 1094.5095
2235 1095.5339 1094.5266
2236 548.7501 1095.4856
2237 548.7530 1095.4914
2238 1096.5232 1095.5159
2240 1096.5341 1095.5268
2241 1097.0243 1096.0170
2242 1097.4946 1096.4873
2243 1097.5256 1096.5183
2244 1098.4812 1097.4739
2246 1098.5305 1097.5232
2247 1098.5376 1097.5303
2248 1099.5219 1098.5146
2249 1099.5325 1098.5252
2250 1099.5334 1098.5261
2252 550.7528 1099.4910
2253 1100.5284 1099.5211
2254 1100.5370 1099.5297
2256 1101.5154 1100.5081
2257 1101.5237 1100.5164
2258 1101.5277 1100.5204
2259 1101.5389 1100.5316
2261 1101.9932 1100.9859
2262 1102.4961 1101.4888
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