MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2028 1050.5042 1049.4969
2029 1050.5092 1049.5019
2030 1051.4979 1050.4906
2031 1051.4991 1050.4918
2032 1051.4998 1050.4925
2033 1051.5109 1050.5036
2034 1051.5126 1050.5053
2035 1052.4954 1051.4881
2036 1052.5139 1051.5066
2037 1053.4944 1052.4871
2038 1053.4995 1052.4922
2039 1053.5051 1052.4978
2040 1053.5101 1052.5028
2041 1053.5212 1052.5139
2042 527.2907 1052.5668
2043 527.2908 1052.5670
2044 527.7438 1053.4730
2047 1054.5012 1053.4939
2048 1054.5103 1053.5030
2049 1054.5123 1053.5050
2050 1054.5170 1053.5097
2051 1055.4807 1054.4734
2053 1055.5249 1054.5176
2054 528.7390 1055.4634
2055 1056.5049 1055.4976
2056 1056.5063 1055.4990
2059 1057.4860 1056.4787
2060 1057.4969 1056.4896
2061 1057.5145 1056.5072
2063 529.7509 1057.4872
2064 529.7510 1057.4874
2065 1058.5144 1057.5071
2066 1058.5170 1057.5097
2067 1058.5176 1057.5103
2068 1058.5319 1057.5246
2070 1059.4971 1058.4898
2072 1060.5063 1059.4990
2073 1060.5135 1059.5062
2074 1060.5176 1059.5103
2075 1060.5320 1059.5247
2076 1061.4862 1060.4789
2077 1061.5114 1060.5041
2078 1061.5129 1060.5056
2079 1061.5166 1060.5093
2080 1061.5168 1060.5095
2082 531.2786 1060.5426
2084 1062.5105 1061.5032
2085 1062.5165 1061.5092
2086 1062.5228 1061.5155
2088 355.1627 1062.4663
2089 1063.4912 1062.4839
2090 1063.5090 1062.5017
2092 1064.5032 1063.4959
2093 1064.5072 1063.4999
2094 1064.5162 1063.5089
2095 532.7625 1063.5104
2096 532.7733 1063.5320
2097 532.7737 1063.5328
2098 1064.5421 1063.5348
2099 1065.5031 1064.4958
2100 1065.5077 1064.5004
2101 1065.5093 1064.5020
2102 1065.5177 1064.5104
2103 533.3312 1064.6478
2104 533.7614 1065.5082
2105 1066.5170 1065.5097
2106 1066.5194 1065.5121
2107 1067.0045 1065.9972
2108 1067.5005 1066.4932
2109 1067.5083 1066.5010
2111 534.2690 1066.5234
2112 1068.5043 1067.4970
2113 1068.5084 1067.5011
2114 1068.5104 1067.5031
2115 1068.5132 1067.5059
2116 1068.5177 1067.5104
2117 1068.5239 1067.5166
2118 1069.5135 1068.5062
2119 1069.5197 1068.5124
2120 1070.4988 1069.4915
2121 1070.5161 1069.5088
2122 1070.5170 1069.5097
2123 1071.5089 1070.5016
2124 1071.5109 1070.5036
2125 1071.5144 1070.5071
2126 1071.5242 1070.5169
2127 1072.5087 1071.5014
2128 536.7683 1071.5220
2129 1072.5319 1071.5246
2130 1073.5078 1072.5005
2131 1073.5156 1072.5083
2132 537.7476 1073.4806
2133 1074.4998 1073.4925
2134 1074.5013 1073.4940
2135 1074.5110 1073.5037
2136 1074.5135 1073.5062
2137 1074.5204 1073.5131
2138 1075.5024 1074.4951
2139 1075.5206 1074.5133
2140 1075.5223 1074.5150
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