MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1797 500.7901 999.5656
1798 1001.4786 1000.4713
1799 1001.4818 1000.4745
1800 1001.4867 1000.4794
1801 1001.4918 1000.4845
1802 1002.4837 1001.4764
1803 1002.4862 1001.4789
1804 1002.4875 1001.4802
1805 1002.4901 1001.4828
1806 335.4756 1003.4050
1807 1004.4637 1003.4564
1808 1004.4772 1003.4699
1809 1004.4854 1003.4781
1810 1004.4908 1003.4835
1811 1004.4912 1003.4839
1812 1004.4935 1003.4862
1813 1005.4777 1004.4704
1814 1005.4784 1004.4711
1815 1005.4835 1004.4762
1816 1005.4879 1004.4806
1819 1006.4771 1005.4698
1820 1006.4861 1005.4788
1821 503.7520 1005.4894
1822 1006.4976 1005.4903
1824 1007.4764 1006.4691
1825 1007.4781 1006.4708
1826 1007.4892 1006.4819
1828 1007.5173 1006.5100
1829 504.2624 1006.5102
1830 1007.5179 1006.5106
1831 504.2634 1006.5122
1832 1007.7975 1006.7902
1833 504.7261 1007.4376
1834 1008.4774 1007.4701
1835 1008.4858 1007.4785
1836 504.7466 1007.4786
1837 1008.4906 1007.4833
1839 504.8070 1007.5994
1840 1009.4858 1008.4785
1843 1010.4795 1009.4722
1844 1010.4799 1009.4726
1845 1010.4799 1009.4726
1846 1010.4921 1009.4848
1847 506.7345 1011.4544
1848 1012.4622 1011.4549
1849 506.7392 1011.4638
1850 1012.4775 1011.4702
1851 1012.4811 1011.4738
1852 1012.4971 1011.4898
1853 1013.4898 1012.4825
1854 1013.4938 1012.4865
1855 1013.4938 1012.4865
1856 1014.4816 1013.4743
1857 1014.4821 1013.4748
1858 1014.4848 1013.4775
1859 1014.4976 1013.4903
1860 1014.5016 1013.4943
1861 1015.4778 1014.4705
1862 1015.4886 1014.4813
1863 1015.4922 1014.4849
1866 1016.4960 1015.4887
1868 1017.4754 1016.4681
1869 1017.4849 1016.4776
1870 1017.4952 1016.4879
1871 1018.4742 1017.4669
1872 1018.4771 1017.4698
1873 1018.4775 1017.4702
1875 1018.4829 1017.4756
1876 1018.4856 1017.4783
1877 1018.4888 1017.4815
1878 1018.4914 1017.4841
1879 1018.5018 1017.4945
1880 510.2245 1018.4344
1882 1019.4941 1018.4868
1883 1019.4967 1018.4894
1884 510.2733 1018.5320
1885 1020.4769 1019.4696
1886 1020.4781 1019.4708
1887 1020.4844 1019.4771
1888 1020.4895 1019.4822
1889 1020.4930 1019.4857
1890 511.2108 1020.4070
1894 341.4872 1021.4398
1895 511.7273 1021.4400
1896 1022.4800 1021.4727
1897 1022.4906 1021.4833
1898 1022.4933 1021.4860
1899 1022.5204 1021.5131
1901 512.2468 1022.4790
1902 512.2475 1022.4804
1903 1023.4882 1022.4809
1904 1023.4945 1022.4872
1905 1023.4959 1022.4886
1906 1023.5082 1022.5009
1907 512.7437 1023.4728
1908 1024.4948 1023.4875
1909 1024.4956 1023.4883
1911 1025.4783 1024.4710
1912 1025.4811 1024.4738
1913 1025.4814 1024.4741
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