MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1676 971.4727 970.4654
1677 486.7266 971.4386
1678 972.4483 971.4410
1679 972.4553 971.4480
1680 972.4630 971.4557
1681 972.4662 971.4589
1682 972.4740 971.4667
1683 972.4751 971.4678
1684 972.4785 971.4712
1685 972.4800 971.4727
1686 974.4615 973.4542
1687 975.4639 974.4566
1688 975.4741 974.4668
1689 975.4776 974.4703
1690 488.2688 974.5230
1691 975.5309 974.5236
1693 488.7653 975.5160
1694 977.4859 976.4786
1695 978.4737 977.4664
1696 978.4886 977.4813
1697 978.4916 977.4843
1699 979.4487 978.4414
1700 979.4619 978.4546
1701 979.4724 978.4651
1703 980.4700 979.4627
1704 980.4728 979.4655
1705 980.4734 979.4661
1706 980.4739 979.4666
1707 980.4780 979.4707
1708 980.4849 979.4776
1709 980.4859 979.4786
1710 981.4638 980.4565
1711 491.2368 980.4590
1712 981.4760 980.4687
1714 982.4674 981.4601
1715 982.4712 981.4639
1716 982.4871 981.4798
1717 983.4579 982.4506
1718 983.4720 982.4647
1719 983.4766 982.4693
1720 983.4880 982.4807
1722 984.4698 983.4625
1723 984.4758 983.4685
1724 984.5004 983.4931
1725 985.4700 984.4627
1726 493.2390 984.4634
1727 985.4725 984.4652
1728 985.4772 984.4699
1729 985.4773 984.4700
1730 985.4930 984.4857
1733 986.4759 985.4686
1734 986.4794 985.4721
1735 986.4806 985.4733
1736 986.5222 985.5149
1737 986.8096 985.8023
1738 987.4643 986.4570
1739 987.4762 986.4689
1741 988.4709 987.4636
1742 988.4786 987.4713
1743 988.5014 987.4941
1744 989.4774 988.4701
1745 989.9757 988.9684
1746 330.8123 989.4151
1747 990.4793 989.4720
1748 990.9771 989.9698
1749 991.4674 990.4601
1750 991.4745 990.4672
1751 991.4808 990.4735
1752 991.4839 990.4766
1753 991.4918 990.4845
1754 991.5237 990.5164
1755 991.5241 990.5168
1757 496.2664 990.5182
1759 496.7619 991.5092
1760 496.7630 991.5114
1761 496.7632 991.5118
1762 496.7633 991.5120
1763 496.7638 991.5130
1764 993.4747 992.4674
1765 994.4886 993.4813
1766 994.4906 993.4833
1767 995.4669 994.4596
1768 995.4794 994.4721
1769 995.4869 994.4796
1770 995.4926 994.4853
1773 996.4788 995.4715
1775 996.4800 995.4727
1777 996.4886 995.4813
1778 996.4908 995.4835
1784 997.4794 996.4721
1785 499.7038 997.3930
1786 998.4628 997.4555
1787 998.4698 997.4625
1788 998.4725 997.4652
1789 998.4794 997.4721
1790 998.4980 997.4907
1793 999.4788 998.4715
1794 999.9924 998.9851
1795 1000.4723 999.4650
1796 1000.4854 999.4781
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