MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1555 471.2208 940.4270
1556 941.4437 940.4364
1557 471.7108 941.4070
1558 942.4599 941.4526
1559 942.4669 941.4596
1560 942.4689 941.4616
1561 942.4696 941.4623
1562 942.4698 941.4625
1563 472.2258 942.4370
1564 943.4477 942.4404
1565 943.4478 942.4405
1566 944.4402 943.4329
1567 944.4592 943.4519
1568 944.4635 943.4562
1570 945.4471 944.4398
1571 945.4559 944.4486
1572 945.4680 944.4607
1573 946.4580 945.4507
1574 946.4622 945.4549
1575 473.7354 945.4562
1576 473.9749 945.9352
1577 947.4488 946.4415
1579 474.2294 946.4442
1580 947.4586 946.4513
1582 947.4668 946.4595
1583 947.4692 946.4619
1585 948.4457 947.4384
1586 948.4578 947.4505
1587 948.5034 947.4961
1588 949.4547 948.4474
1589 949.4553 948.4480
1593 475.2604 948.5062
1594 950.4658 949.4585
1595 950.4677 949.4604
1596 950.4677 949.4604
1598 475.7388 949.4630
1600 951.4236 950.4163
1601 476.2158 950.4170
1603 951.4492 950.4419
1604 951.4496 950.4423
1605 951.4578 950.4505
1606 952.4492 951.4419
1607 952.4584 951.4511
1608 952.4680 951.4607
1609 953.4340 952.4267
1610 953.4551 952.4478
1611 953.4565 952.4492
1612 953.4581 952.4508
1613 954.4510 953.4437
1614 954.4544 953.4471
1615 954.4548 953.4475
1617 955.4458 954.4385
1618 955.4470 954.4397
1620 956.4650 955.4577
1623 957.4570 956.4497
1624 957.4673 956.4600
1625 958.4609 957.4536
1626 958.4662 957.4589
1627 959.4557 958.4484
1628 959.4597 958.4524
1629 480.7676 959.5206
1631 961.4424 960.4351
1632 961.4536 960.4463
1633 961.4537 960.4464
1634 961.4611 960.4538
1635 961.4694 960.4621
1636 961.4990 960.4917
1637 962.4612 961.4539
1638 963.4594 962.4521
1639 963.4604 962.4531
1640 963.4672 962.4599
1641 964.4621 963.4548
1642 964.4755 963.4682
1643 965.4546 964.4473
1644 965.4695 964.4622
1645 965.4721 964.4648
1646 483.2571 964.4996
1647 966.4581 965.4508
1648 966.4601 965.4528
1650 966.4653 965.4580
1651 483.7368 965.4590
1653 966.4732 965.4659
1655 484.2132 966.4118
1656 967.4644 966.4571
1657 484.2461 966.4776
1659 484.7352 967.4558
1660 968.4669 967.4596
1661 484.7378 967.4610
1662 968.4698 967.4625
1663 968.4728 967.4655
1664 968.4761 967.4688
1666 968.4810 967.4737
1667 968.4854 967.4781
1668 969.4648 968.4575
1669 969.4660 968.4587
1670 969.4827 968.4754
1671 970.4755 969.4682
1672 485.7466 969.4786
1674 971.4620 970.4547
1675 971.4666 970.4593
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