MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1991 1042.5189 1041.5116 1041.5277 -15.4 0 8 0.85 +1Score > 20 indicates identity
Score > 19 indicates homology		 MEIHSNLAK
1040 367.1855 732.3564 732.3442 16.7 1 8 0.42 +1Score > 22 indicates identity
Score > 16 indicates homology		 LGWDKN + Deamidated (NQ)
1384 447.2336 892.4526 892.4364 18.2 0 8 1 +1Score > 22 indicates identity
Score > 20 indicates homology		 IPQFMEK + Deamidated (NQ)
1053 370.7011 739.3876 739.3864 1.63 0 8 0.65 +1Score > 18 indicates identity VDHELK
3028 752.8951 1503.7756 1503.7722 2.31 0 8 0.35 +1Score > 23 indicates identity
Score > 15 indicates homology		 VSFWEETILQPR
2985 737.8649 1473.7152 1473.7212 -4.03 1 8 0.57 +1Score > 22 indicates identity
Score > 18 indicates homology		 KDHGDVSNQLYAK
2793 653.8458 1305.6770 1305.7003 -17.8 0 7 0.2 +1Score > 23 indicates identity
Score > 13 indicates homology		 MVASTTVPYLPK
1184 406.2087 810.4028 810.4064 -4.44 0 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 WAPYFK
1974 519.7480 1037.4814 1037.4890 -7.29 0 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 GSIDEQHPR
806 555.3145 554.3072 554.3176 -18.8 0 7 0.31 +1Score > 15 indicates identity ITGHK
2057 528.7703 1055.5260 1055.5247 1.26 1 7 0.95 +1Score > 20 indicates identity EKGLTHEDK
2532 583.2845 1164.5544 1164.5676 -11.3 0 7 0.52 +1Score > 23 indicates identity
Score > 17 indicates homology		 NNLHAEGWPK
864 311.1537 620.2928 620.2918 1.65 0 7 0.81 +1Score > 20 indicates identity
Score > 19 indicates homology		 FDPSR
802 545.3109 544.3036 544.2969 12.4 0 7 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 GIAER
2930 723.8658 1445.7170 1445.7085 5.89 0 7 1 +1Score > 22 indicates identity
Score > 20 indicates homology		 SHGAVLPSYCSLR
859 309.6662 617.3178 617.3133 7.43 1 7 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 KNGNGK + Deamidated (NQ)
1732 493.7312 985.4478 985.4651 -17.5 0 7 0.61 +1Score > 18 indicates identity EDALMVHR + Oxidation (M)
855 308.6872 615.3598 615.3592 1.12 0 7 1 +1Score > 25 indicates identity
Score > 20 indicates homology		 AVIEGK
1962 518.2840 1034.5534 1034.5396 13.3 0 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 VYSINNTPK
2803 660.3344 1318.6542 1318.6703 -12.2 1 7 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 RSNMFVPLPNK + Deamidated (NQ); Oxidation (M)
1045 368.1754 734.3362 734.3460 -13.2 0 7 1 +1Score > 20 indicates identity
Score > 20 indicates homology		 NFQNGR
3999 726.7080 2177.1022 2177.1249 -10.4 1 7 1 +1Score > 22 indicates identity
Score > 20 indicates homology		 MNSKEALQILNLTENTLTK + Deamidated (NQ); Oxidation (M)
919 655.3774 654.3701 654.3701 0.081 0 7 0.27 +1Score > 14 indicates identity SVPAPGK
1104 384.2079 766.4012 766.3861 19.8 0 7 0.63 +1Score > 21 indicates identity
Score > 18 indicates homology		 QLNYTK + Deamidated (NQ)
3887 705.3387 2112.9943 2113.0044 -4.81 1 7 0.39 +1Score > 22 indicates identity
Score > 16 indicates homology		 SPVGTRLQINSQNCIHCK + 2 Deamidated (NQ)
2931 723.8659 1445.7172 1445.7085 6.03 0 7 1 +1Score > 22 indicates identity
Score > 20 indicates homology		 SHGAVLPSYCSLR
2382 567.2900 1132.5654 1132.5798 -12.7 1 7 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 LDPKTAETMK
2719 414.5511 1240.6315 1240.6339 -1.99 0 7 0.33 +1Score > 22 indicates identity
Score > 15 indicates homology		 YVLYGVSTPNK + Deamidated (NQ)
2773 640.8179 1279.6212 1279.6329 -9.13 1 7 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 KTSQLMEEIGK + Deamidated (NQ); Oxidation (M)
3977 722.3446 2164.0120 2163.9953 7.68 0 7 0.24 +1Score > 22 indicates identity
Score > 13 indicates homology		 NMTNSAASLATSPTSNQLNPK + 2 Deamidated (NQ); Oxidation (M)
3156 789.9162 1577.8178 1577.8089 5.64 0 7 1 +1Score > 23 indicates identity
Score > 19 indicates homology		 YSLPPQTIQDLFR + Deamidated (NQ)
2841 451.8979 1352.6719 1352.6725 -0.43 0 7 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 VANGNNVVQFYK + Deamidated (NQ)
1341 879.4598 878.4525 878.4538 -1.45 0 7 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 YFPDLPK
938 334.1928 666.3710 666.3813 -15.4 0 7 0.55 +1Score > 17 indicates identity GLPPQR
3074 765.8776 1529.7406 1529.7508 -6.62 0 7 0.51 +1Score > 22 indicates identity
Score > 17 indicates homology		 CVNETLDRPLADK
1074 376.1909 750.3672 750.3548 16.6 0 7 0.23 +1Score > 22 indicates identity
Score > 13 indicates homology		 QPYESK
1102 767.4055 766.3982 766.3973 1.17 0 7 0.69 +1Score > 20 indicates identity
Score > 18 indicates homology		 KPEQHK + Deamidated (NQ)
1000 705.3186 704.3113 704.3089 3.40 0 7 0.86 +1Score > 19 indicates identity GNSGVDR + Deamidated (NQ)
954 338.1771 674.3396 674.3388 1.32 0 7 0.3 +1Score > 22 indicates identity
Score > 14 indicates homology		 NWIDK
2266 552.2559 1102.4972 1102.5142 -15.4 0 7 0.97 +1Score > 19 indicates identity EGGETQEKPK + Deamidated (NQ)
1064 372.2067 742.3988 742.4086 -13.1 1 7 0.69 +1Score > 18 indicates identity KANPTGR
2875 695.8739 1389.7332 1389.7252 5.80 1 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 IRELLNFNDEK
1137 785.3788 784.3715 784.3576 17.7 0 7 0.89 +1Score > 19 indicates identity GANGHSSR
2965 729.3849 1456.7552 1456.7522 2.13 1 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 IDGLAEKDDLVNR
2083 1062.4952 1061.4879 1061.4989 -10.3 1 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 LSDEDAEKR
3157 789.9163 1577.8180 1577.8089 5.77 0 7 0.54 +1Score > 23 indicates identity
Score > 16 indicates homology		 YSLPPQTIQDLFR + Deamidated (NQ)
1394 898.3906 897.3833 897.3716 13.1 0 7 0.64 +1Score > 17 indicates identity ADSGYEEK
829 586.3203 585.3130 585.3235 -17.8 0 7 0.91 +1Score > 19 indicates identity AVSPGR
949 673.3521 672.3448 672.3555 -15.9 0 7 0.9 +1Score > 19 indicates identity VAVGNGR + Deamidated (NQ)
1842 505.2727 1008.5308 1008.5314 -0.53 1 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 MVDKIFEK
1740 494.2557 986.4968 986.5032 -6.47 0 6 0.5 +1Score > 22 indicates identity
Score > 16 indicates homology		 QLGNNEIAK + Deamidated (NQ)
2741 630.3000 1258.5854 1258.5902 -3.75 1 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology		 QNLRQADDGNK + Deamidated (NQ)
1113 772.4228 771.4155 771.4239 -10.8 0 6 0.26 +1Score > 20 indicates identity
Score > 13 indicates homology		 VAQNAIR + Deamidated (NQ)
1008 355.6818 709.3490 709.3469 3.04 0 6 0.47 +1Score > 20 indicates identity
Score > 16 indicates homology		 FGMDLK
3104 773.8999 1545.7852 1545.7610 15.7 0 6 0.43 +1Score > 22 indicates identity
Score > 15 indicates homology		 QPCSPIIQHTHVK + 2 Deamidated (NQ)
2839 677.3411 1352.6676 1352.6460 16.0 1 6 1 +1Score > 23 indicates identity
Score > 19 indicates homology		 GKESLQDVYDAK + Deamidated (NQ)
1484 463.2226 924.4306 924.4375 -7.41 0 6 0.95 +1Score > 20 indicates identity
Score > 19 indicates homology		 QLTQFCK + Deamidated (NQ)
1058 742.4113 741.4040 741.4021 2.62 0 6 0.76 +1Score > 18 indicates identity AAEGPVAK
2876 695.8756 1389.7366 1389.7252 8.25 1 6 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 IRELLNFNDEK
3189 800.9018 1599.7890 1599.7780 6.90 0 6 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 EFPAINIPDNVNEK + Deamidated (NQ)
831 588.2993 587.2920 587.3027 -18.2 0 6 0.99 +1Score > 19 indicates identity NLDAR
1963 518.2846 1034.5546 1034.5396 14.5 0 6 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 VYSINNTPK
1296 431.2146 860.4146 860.4239 -10.8 0 6 0.89 +1Score > 22 indicates identity
Score > 18 indicates homology		 GANQGTAIK + 2 Deamidated (NQ)
1539 938.4228 937.4155 937.4215 -6.38 0 6 1.2 +1Score > 19 indicates identity WAVNTLMS + Deamidated (NQ); Oxidation (M)
1548 470.2358 938.4570 938.4570 0.083 0 6 0.69 +1Score > 20 indicates identity
Score > 17 indicates homology		 QENLHNGK
2608 1182.5778 1181.5705 1181.5815 -9.33 0 6 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 ISNQIIDSYK + 2 Deamidated (NQ)
3107 773.9070 1545.7994 1545.8012 -1.11 1 6 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 VASHKNSHNPSLVR + Deamidated (NQ)
2622 1184.5548 1183.5475 1183.5404 6.04 1 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology		 STLRCNYNR + Deamidated (NQ)
1549 939.4653 938.4580 938.4457 13.1 0 6 0.94 +1Score > 20 indicates identity
Score > 18 indicates homology		 ANESSLYR
916 655.3432 654.3359 654.3489 -19.9 0 6 0.88 +1Score > 18 indicates identity GLFYR
2709 617.2936 1232.5726 1232.5893 -13.5 0 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 NQPGLSMMLNK + Deamidated (NQ)
1183 810.3637 809.3564 809.3555 1.12 0 6 0.97 +1Score > 18 indicates identity EGANYQK + Deamidated (NQ)
1204 411.7276 821.4406 821.4317 10.9 1 6 0.35 +1Score > 21 indicates identity
Score > 14 indicates homology		 ETMKLGK + Oxidation (M)
1187 406.2253 810.4360 810.4236 15.4 0 6 1.3 +1Score > 19 indicates identity LQNDPPK
2537 1166.5730 1165.5657 1165.5866 -17.9 0 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 SLEDILNAYK + Deamidated (NQ)
3277 832.3828 1662.7510 1662.7631 -7.25 1 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 QVATNEMAALRNGGGR + 3 Deamidated (NQ); Oxidation (M)
3105 773.9019 1545.7892 1545.8012 -7.71 1 6 0.51 +1Score > 22 indicates identity
Score > 15 indicates homology		 VASHKNSHNPSLVR + Deamidated (NQ)
2358 1126.5155 1125.5082 1125.5302 -19.5 0 6 0.37 +1Score > 20 indicates identity
Score > 14 indicates homology		 GSSNSLLEYR + Deamidated (NQ)
1164 800.3790 799.3717 799.3864 -18.4 0 6 0.7 +1Score > 17 indicates identity YNYAIR + Deamidated (NQ)
941 669.3322 668.3249 668.3129 17.9 0 6 1.2 +1Score > 19 indicates identity SASGYGK
1285 429.7119 857.4092 857.4171 -9.12 0 6 0.82 +1Score > 17 indicates identity YLSNIFT + Deamidated (NQ)
1616 477.7494 953.4842 953.4971 -13.4 1 6 0.55 +1Score > 20 indicates identity
Score > 16 indicates homology		 KSGWVSYK
2939 723.8737 1445.7328 1445.7085 16.8 0 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 SHGAVLPSYCSLR
2012 1048.5187 1047.5114 1047.5322 -19.9 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 RTGSHTHPR
2870 695.3431 1388.6716 1388.6531 13.3 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 NPSRSIADSAQNK + 2 Deamidated (NQ)
2454 1149.5690 1148.5617 1148.5826 -18.1 0 6 0.4 +1Score > 22 indicates identity
Score > 14 indicates homology		 GAQVNISANFK + Deamidated (NQ)
1359 442.7282 883.4418 883.4399 2.18 1 6 1.3 +1Score > 19 indicates identity KDIDEHK
2046 1054.4807 1053.4734 1053.4839 -9.96 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 TDNQYTRR + Deamidated (NQ)
1569 472.7390 943.4634 943.4723 -9.39 0 6 1.4 +1Score > 19 indicates identity LGEGQIDGR
2593 589.8148 1177.6150 1177.6311 -13.6 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 LSRLVGMGMAK + Oxidation (M)
799 542.2943 541.2870 541.2972 -18.8 0 5 0.33 +1Score > 13 indicates identity QPAAR
1168 400.7174 799.4202 799.4301 -12.3 0 5 1.1 +1Score > 18 indicates identity LLGGGGGGGR
2672 603.3134 1204.6122 1204.6313 -15.8 0 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 LLDHSSQLHR
1599 475.7454 949.4762 949.4716 4.91 1 5 0.43 +1Score > 22 indicates identity
Score > 14 indicates homology		 NSNASSKLK + 2 Deamidated (NQ)
2804 660.3364 1318.6582 1318.6703 -9.17 1 5 0.38 +1Score > 23 indicates identity
Score > 14 indicates homology		 RSNMFVPLPNK + Deamidated (NQ); Oxidation (M)
853 615.3472 614.3399 614.3388 1.87 0 5 1.1 +1Score > 18 indicates identity SVQPGK
1152 397.6880 793.3614 793.3640 -3.21 0 5 0.74 +1Score > 21 indicates identity
Score > 17 indicates homology		 MAISDGGK + Oxidation (M)
2817 667.3219 1332.6292 1332.6310 -1.31 0 5 1 +1Score > 23 indicates identity
Score > 18 indicates homology		 GPSVEDVEQAFR
1108 385.6973 769.3800 769.3718 10.7 1 5 1.1 +1Score > 18 indicates identity GNKANHK + 2 Deamidated (NQ)
1210 413.7065 825.3984 825.4021 -4.42 0 5 1.2 +1Score > 19 indicates identity QIDFFR + Deamidated (NQ)
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