MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1312 434.1959 866.3772
1313 867.3853 866.3780
1314 867.4156 866.4083
1315 434.6971 867.3796
1316 868.4175 867.4102
1317 868.4246 867.4173
1318 868.4265 867.4192
1320 869.4114 868.4041
1321 870.4246 869.4173
1322 871.4255 870.4182
1323 872.0861 871.0788
1324 872.4200 871.4127
1325 872.4218 871.4145
1326 873.4262 872.4189
1327 874.4114 873.4041
1328 874.4183 873.4110
1329 874.4272 873.4199
1330 875.4296 874.4223
1331 438.2534 874.4922
1333 875.5045 874.4972
1334 876.4297 875.4224
1335 876.4297 875.4224
1336 438.7386 875.4626
1338 878.4048 877.3975
1339 439.7285 877.4424
1342 440.2571 878.4996
1343 880.4190 879.4117
1344 880.4401 879.4328
1345 881.4260 880.4187
1346 441.7021 881.3896
1347 882.4006 881.3933
1348 882.4153 881.4080
1349 882.4218 881.4145
1351 441.7385 881.4624
1352 882.4699 881.4626
1353 883.3809 882.3736
1354 883.4331 882.4258
1356 884.4175 883.4102
1357 884.4362 883.4289
1360 443.1935 884.3724
1362 886.4299 885.4226
1363 886.4324 885.4251
1365 886.4866 885.4793
1366 886.4896 885.4823
1367 444.2147 886.4148
1368 887.4227 886.4154
1369 887.4268 886.4195
1370 444.2222 886.4298
1371 888.4319 887.4246
1372 889.4341 888.4268
1373 889.5277 888.5204
1374 890.4307 889.4234
1375 890.4457 889.4384
1376 891.4309 890.4236
1377 891.4386 890.4313
1379 891.4449 890.4376
1380 892.4488 891.4415
1381 892.4641 891.4568
1383 893.4533 892.4460
1385 447.2657 892.5168
1386 894.4164 893.4091
1387 894.4276 893.4203
1388 895.3981 894.3908
1389 448.2030 894.3914
1390 895.4480 894.4407
1392 896.4351 895.4278
1393 897.4318 896.4245
1396 449.6993 897.3840
1397 898.4255 897.4182
1398 898.4392 897.4319
1399 899.4371 898.4298
1400 899.4399 898.4326
1401 899.4437 898.4364
1402 899.4487 898.4414
1403 899.4584 898.4511
1404 450.2341 898.4536
1405 450.2490 898.4834
1407 450.2517 898.4888
1408 900.4366 899.4293
1409 450.7249 899.4352
1410 900.4448 899.4375
1411 900.4498 899.4425
1412 900.4529 899.4456
1413 901.4367 900.4294
1414 901.9297 900.9224
1415 902.4313 901.4240
1416 902.4476 901.4403
1418 903.4357 902.4284
1419 903.4492 902.4419
1420 904.4258 903.4185
1421 452.7487 903.4828
1422 904.4955 903.4882
1423 905.4391 904.4318
1424 905.4498 904.4425
1425 905.4754 904.4681
1426 453.2417 904.4688
1427 905.9458 904.9385
1428 453.7200 905.4254
1430 906.4361 905.4288
1431 906.4418 905.4345
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