MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1177 803.3812 802.3739
1178 805.3958 804.3885
1180 808.9099 807.9026
1181 809.3881 808.3808
1182 809.4037 808.3964
1186 406.2251 810.4356
1188 813.4122 812.4049
1190 814.3708 813.3635
1191 814.3798 813.3725
1192 814.3799 813.3726
1193 814.3978 813.3905
1195 816.9167 815.9094
1196 817.3969 816.3896
1197 818.4031 817.3958
1198 818.4114 817.4041
1199 818.4127 817.4054
1201 411.2176 820.4206
1202 411.2361 820.4576
1203 822.3959 821.3886
1206 412.7176 823.4206
1207 824.9171 823.9098
1208 825.3752 824.3679
1209 826.3992 825.3919
1211 414.2175 826.4204
1212 828.3730 827.3657
1213 828.3736 827.3663
1214 828.4419 827.4346
1215 828.4426 827.4353
1216 830.3930 829.3857
1217 830.4037 829.3964
1218 417.1986 832.3826
1220 834.4059 833.3986
1221 835.4009 834.3936
1222 835.4040 834.3967
1224 418.6769 835.3392
1227 837.5576 836.5503
1228 838.4785 837.4712
1229 838.4835 837.4762
1230 840.4260 839.4187
1233 840.8775 839.8702
1234 841.3683 840.3610
1235 841.3697 840.3624
1237 841.4153 840.4080
1239 842.3950 841.3877
1240 842.4054 841.3981
1241 842.4150 841.4077
1242 421.7226 841.4306
1243 421.7228 841.4310
1245 421.7407 841.4668
1246 842.9098 841.9025
1247 843.3856 842.3783
1248 843.4150 842.4077
1249 422.2136 842.4126
1250 422.7031 843.3916
1251 844.4042 843.3969
1252 844.4264 843.4191
1253 845.4297 844.4224
1256 846.4236 845.4163
1259 848.4077 847.4004
1260 848.4239 847.4166
1261 425.1967 848.3788
1262 425.2094 848.4042
1263 851.3904 850.3831
1264 426.6741 851.3336
1265 853.4075 852.4002
1266 853.4360 852.4287
1267 427.2300 852.4454
1271 427.2491 852.4836
1272 854.4122 853.4049
1273 427.7105 853.4064
1274 854.4158 853.4085
1275 854.4199 853.4126
1276 855.3837 854.3764
1278 855.4158 854.4085
1280 856.4053 855.3980
1282 857.4015 856.3942
1283 857.4203 856.4130
1287 858.4213 857.4140
1288 858.4324 857.4251
1289 429.7217 857.4288
1290 859.3794 858.3721
1291 430.2116 858.4086
1292 860.4008 859.3935
1293 860.4160 859.4087
1294 861.4019 860.3946
1295 861.4056 860.3983
1297 861.4265 860.4192
1298 861.4412 860.4339
1299 863.3342 862.3269
1300 863.4039 862.3966
1301 863.4162 862.4089
1302 863.4600 862.4527
1303 432.2412 862.4678
1304 864.3273 863.3200
1306 865.4074 864.4001
1307 865.4399 864.4326
1308 866.4220 865.4147
1309 866.9125 865.9052
1310 867.0880 866.0807
1311 867.3837 866.3764
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