MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1015 718.6581 717.6508
1016 718.9941 717.9868
1017 720.3683 719.3610
1018 721.3158 720.3085
1020 361.7015 721.3884
1023 723.8713 722.8640
1024 362.7091 723.4036
1026 362.7093 723.4040
1027 725.3946 724.3873
1028 726.3428 725.3355
1029 726.3429 725.3356
1030 726.3430 725.3357
1031 363.6808 725.3470
1032 728.3225 727.3152
1033 728.3965 727.3892
1035 728.8369 727.8296
1037 730.3376 729.3303
1038 730.3380 729.3307
1041 367.1907 732.3668
1042 367.1911 732.3676
1043 734.8552 733.8479
1044 735.3299 734.3226
1046 736.3648 735.3575
1047 737.8507 736.8434
1048 369.6933 737.3720
1049 739.3705 738.3632
1050 740.3542 739.3469
1051 740.3683 739.3610
1054 371.1802 740.3458
1059 742.4113 741.4040
1061 743.3613 742.3540
1062 743.3973 742.3900
1063 743.4058 742.3985
1066 744.3171 743.3098
1067 744.3528 743.3455
1068 744.3541 743.3468
1071 745.8605 744.8532
1072 748.3173 747.3100
1073 374.6909 747.3672
1076 752.3803 751.3730
1077 753.3699 752.3626
1079 755.3784 754.3711
1080 755.4069 754.3996
1081 756.3530 755.3457
1082 756.3538 755.3465
1083 757.4138 756.4065
1084 757.4187 756.4114
1085 379.2166 756.4186
1086 379.2175 756.4204
1087 379.6940 757.3734
1089 759.8805 758.8732
1090 760.3605 759.3532
1091 762.3392 761.3319
1093 764.3574 763.3501
1094 764.4329 763.4256
1095 765.3892 764.3819
1096 765.3894 764.3821
1098 767.0438 766.0365
1099 767.3688 766.3615
1105 768.7015 767.6942
1107 770.3846 769.3773
1111 772.3480 771.3407
1114 773.3997 772.3924
1116 775.8718 774.8645
1119 390.2124 778.4102
1120 390.2127 778.4108
1121 780.8813 779.8740
1122 391.1906 780.3666
1123 781.3765 780.3692
1124 781.3825 780.3752
1126 391.2017 780.3888
1127 391.2151 780.4156
1128 782.4050 781.3977
1130 783.3704 782.3631
1133 783.4357 782.4284
1135 783.4368 782.4295
1136 392.2224 782.4302
1138 393.1941 784.3736
1139 393.1982 784.3818
1140 786.3653 785.3580
1144 787.3883 786.3810
1145 787.8956 786.8883
1146 788.3778 787.3705
1147 791.3956 790.3883
1150 793.9265 792.9192
1154 397.6884 793.3622
1155 794.3926 793.3853
1156 794.3937 793.3864
1157 795.4116 794.4043
1158 398.2098 794.4050
1161 797.3798 796.3725
1162 799.3580 798.3507
1163 400.2195 798.4244
1167 400.7171 799.4196
1170 800.4280 799.4207
1171 801.3751 800.3678
1173 401.1969 800.3792
1174 801.3980 800.3907
1175 801.4088 800.4015
1176 802.4332 801.4259
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