MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
866 624.3364 623.3291
867 624.3365 623.3292
868 625.2952 624.2879
869 625.3426 624.3353
870 313.1750 624.3354
872 626.2772 625.2699
873 626.2894 625.2821
874 626.3531 625.3458
875 629.2897 628.2824
876 629.2906 628.2833
879 634.2818 633.2745
880 317.6639 633.3132
883 635.3059 634.2986
884 637.3214 636.3141
887 320.1768 638.3390
889 320.1770 638.3394
891 640.3685 639.3612
892 640.3692 639.3619
893 321.1723 640.3300
895 321.1734 640.3322
897 321.1849 640.3552
898 321.1850 640.3554
900 643.2614 642.2541
901 644.3284 643.3211
907 648.3107 647.3034
908 648.3723 647.3650
910 326.1827 650.3508
911 327.1848 652.3550
912 327.1908 652.3670
915 655.3420 654.3347
917 655.3748 654.3675
918 328.1923 654.3700
921 656.8190 655.8117
928 665.3624 664.3551
929 665.3627 664.3554
930 333.1908 664.3670
931 333.2029 664.3912
932 667.3060 666.2987
933 667.3304 666.3231
935 667.3533 666.3460
936 334.1804 666.3462
937 334.1804 666.3462
939 334.7032 667.3918
940 669.3228 668.3155
942 670.3160 669.3087
943 670.3178 669.3105
944 671.2993 670.2920
945 671.3015 670.2942
946 671.4228 670.4155
947 673.3150 672.3077
948 673.3521 672.3448
950 337.1797 672.3448
951 337.1799 672.3452
953 337.6503 673.2860
955 675.4172 674.4099
956 675.4174 674.4101
957 676.3077 675.3004
958 676.3107 675.3034
960 678.3224 677.3151
961 681.3589 680.3516
962 681.3687 680.3614
964 341.1886 680.3626
965 341.2002 680.3858
966 341.2006 680.3866
967 341.2008 680.3870
968 681.8137 680.8064
969 682.3311 681.3238
971 341.6867 681.3588
972 342.1902 682.3658
973 683.3751 682.3678
974 683.3790 682.3717
976 685.3544 684.3471
977 687.3314 686.3241
978 688.3873 687.3800
979 689.3232 688.3159
981 690.3494 689.3421
982 346.2033 690.3920
983 693.8418 692.8345
984 694.3386 693.3313
985 348.2080 694.4014
986 696.3328 695.3255
987 696.3336 695.3263
988 697.3149 696.3076
989 349.1980 696.3814
990 349.1982 696.3818
991 350.1749 698.3352
992 699.3466 698.3393
993 701.3475 700.3402
996 702.3207 701.3134
997 702.3211 701.3138
998 702.4130 701.4057
1002 353.6803 705.3460
1003 706.3550 705.3477
1004 708.3717 707.3644
1005 708.3792 707.3719
1006 354.6934 707.3722
1009 711.4172 710.4099
1010 711.4195 710.4122
1012 712.3636 711.3563
1014 717.3386 716.3313
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