MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
735 459.2203 458.2130
736 459.2412 458.2339
737 460.2767 459.2694
738 460.2773 459.2700
739 461.2577 460.2504
740 461.2588 460.2515
741 461.2592 460.2519
742 461.2596 460.2523
743 461.2596 460.2523
744 464.2169 463.2096
746 472.2786 471.2713
747 472.2789 471.2716
748 472.2886 471.2813
749 474.2315 473.2242
750 474.2324 473.2251
751 475.2365 474.2292
752 475.2388 474.2315
753 475.2391 474.2318
754 475.2396 474.2323
755 475.2401 474.2328
756 475.2408 474.2335
757 477.1133 476.1060
758 477.3143 476.3070
759 478.2000 477.1927
760 479.2666 478.2593
761 484.7433 483.7360
762 487.2521 486.2448
763 488.1938 487.1865
764 488.2356 487.2283
765 488.2361 487.2288
766 493.0199 492.0126
767 493.0219 492.0146
768 493.0219 492.0146
769 493.0225 492.0152
770 493.0225 492.0152
771 493.0233 492.0160
772 493.0236 492.0163
773 493.0237 492.0164
774 499.2153 498.2080
775 501.2317 500.2244
776 501.2679 500.2606
777 513.2687 512.2614
778 514.2626 513.2553
780 516.2598 515.2525
781 526.2994 525.2921
782 527.2828 526.2755
784 528.7711 527.7638
785 529.2303 528.2230
786 529.2622 528.2549
787 529.2636 528.2563
788 529.2989 528.2916
790 530.7759 529.7686
792 531.2777 530.2704
793 535.2504 534.2431
794 535.2516 534.2443
795 537.3041 536.2968
796 539.3199 538.3126
797 539.3208 538.3135
798 540.2798 539.2725
800 542.3565 541.3492
803 553.2747 552.2674
804 554.3004 553.2931
805 554.3060 553.2987
807 556.3085 555.3012
808 556.3101 555.3028
810 557.2816 556.2743
811 563.2698 562.2625
812 564.3047 563.2974
813 568.2734 567.2661
814 570.7183 569.7110
815 570.7186 569.7113
816 573.2879 572.2806
817 576.3519 575.3446
818 576.3533 575.3460
819 579.2449 578.2376
820 579.3155 578.3082
821 579.3171 578.3098
822 580.3009 579.2936
823 580.3011 579.2938
824 582.2922 581.2849
825 584.3045 583.2972
828 586.2842 585.2769
830 588.2671 587.2598
832 592.2729 591.2656
833 596.3416 595.3343
834 598.2845 597.2772
837 598.3329 597.3256
838 600.3367 599.3294
839 600.3382 599.3309
841 602.2797 601.2724
842 606.3024 605.2951
843 608.3409 607.3336
844 608.3429 607.3356
845 608.3442 607.3369
846 304.8368 607.6590
847 304.8370 607.6594
848 610.2841 609.2768
849 610.2845 609.2772
850 612.3010 611.2937
863 620.3413 619.3340
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