MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
635 423.2007 422.1934
636 423.2013 422.1940
637 423.2016 422.1943
638 423.2201 422.2128
639 423.2216 422.2143
640 423.2216 422.2143
641 423.2219 422.2146
642 423.2223 422.2150
643 423.2224 422.2151
644 423.2231 422.2158
645 423.2231 422.2158
646 423.2232 422.2159
647 423.2234 422.2161
648 423.2234 422.2161
649 423.2238 422.2165
650 423.2913 422.2840
651 425.2355 424.2282
652 425.2372 424.2299
653 425.2374 424.2301
654 425.2376 424.2303
655 425.2378 424.2305
656 425.2381 424.2308
657 425.2389 424.2316
658 425.2389 424.2316
659 426.2073 425.2000
660 427.2547 426.2474
661 427.2547 426.2474
662 427.7100 426.7027
663 428.2551 427.2478
664 428.2560 427.2487
665 428.2561 427.2488
666 428.2564 427.2491
667 428.2566 427.2493
668 428.2574 427.2501
669 428.2623 427.2550
670 430.2303 429.2230
671 430.2307 429.2234
672 430.3023 429.2950
673 437.2009 436.1936
674 437.2021 436.1948
675 437.2024 436.1951
676 438.1985 437.1912
677 439.2145 438.2072
678 439.2166 438.2093
679 439.2168 438.2095
680 439.2170 438.2097
681 439.2171 438.2098
682 439.2175 438.2102
683 439.2179 438.2106
684 439.2180 438.2107
685 439.2183 438.2110
686 439.2184 438.2111
687 439.2184 438.2111
688 439.2185 438.2112
689 439.7285 438.7212
690 440.2876 439.2803
691 441.2100 440.2027
692 441.2305 440.2232
693 441.2324 440.2251
694 441.2339 440.2266
695 441.2350 440.2277
696 441.2353 440.2280
697 442.2353 441.2280
698 442.2354 441.2281
699 442.2355 441.2282
700 442.2365 441.2292
701 442.2372 441.2299
702 442.2428 441.2355
703 443.2471 442.2398
704 443.2481 442.2408
705 443.2483 442.2410
706 443.2484 442.2411
707 443.2487 442.2414
708 443.2495 442.2422
709 443.2500 442.2427
710 443.2507 442.2434
711 444.2826 443.2753
712 444.2827 443.2754
713 446.2618 445.2545
714 447.2775 446.2702
715 449.1517 448.1444
716 450.1989 449.1916
717 455.2108 454.2035
718 455.2116 454.2043
719 455.2117 454.2044
720 455.2121 454.2048
721 455.2122 454.2049
722 455.2131 454.2058
723 455.2136 454.2063
724 457.2255 456.2182
725 457.2278 456.2205
726 457.2278 456.2205
727 457.2280 456.2207
728 457.2281 456.2208
729 457.2284 456.2211
730 457.2285 456.2212
731 457.2292 456.2219
732 457.2296 456.2223
733 457.2296 456.2223
734 459.1031 458.0958
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