MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
535 388.1827 387.1754
536 389.2396 388.2323
537 391.2312 390.2239
538 391.2881 390.2808
539 391.2882 390.2809
540 391.2887 390.2814
541 391.2890 390.2817
542 391.2891 390.2818
543 393.2463 392.2390
544 393.2473 392.2400
545 393.2473 392.2400
546 393.2475 392.2402
547 393.2494 392.2421
548 393.3009 392.2936
549 393.3014 392.2941
550 393.3015 392.2942
551 393.3016 392.2943
552 393.3021 392.2948
553 393.3025 392.2952
554 393.3027 392.2954
555 393.3027 392.2954
556 393.3027 392.2954
557 393.3028 392.2955
558 393.3029 392.2956
559 393.3030 392.2957
560 395.1928 394.1855
561 395.1936 394.1863
562 395.2263 394.2190
563 395.2263 394.2190
564 395.2267 394.2194
565 395.2269 394.2196
566 397.2060 396.1987
567 397.2064 396.1991
568 397.2065 396.1992
569 397.2065 396.1992
570 397.2070 396.1997
571 397.2075 396.2002
572 397.2087 396.2014
573 397.2094 396.2021
574 397.2775 396.2702
575 399.2215 398.2142
576 399.2229 398.2156
577 399.2233 398.2160
578 399.2236 398.2163
579 400.2323 399.2250
580 400.2326 399.2253
581 401.2214 400.2141
582 401.2376 400.2303
583 402.2107 401.2034
584 402.2469 401.2396
585 405.2091 404.2018
586 405.2109 404.2036
587 405.2109 404.2036
588 405.2110 404.2037
589 405.2115 404.2042
590 406.2064 405.1991
591 407.2094 406.2021
592 407.2243 406.2170
593 407.2263 406.2190
594 407.2264 406.2191
595 407.2265 406.2192
596 407.2267 406.2194
597 407.2271 406.2198
598 407.2276 406.2203
599 407.2277 406.2204
600 407.2278 406.2205
601 409.2425 408.2352
602 409.2427 408.2354
603 409.2432 408.2359
604 409.2435 408.2362
605 409.2435 408.2362
606 409.2438 408.2365
607 410.2442 409.2369
608 410.2452 409.2379
609 410.2456 409.2383
610 411.2208 410.2135
611 411.2216 410.2143
612 412.2609 411.2536
613 412.2614 411.2541
614 412.2617 411.2544
615 412.2617 411.2544
616 413.2731 412.2658
617 413.2738 412.2665
618 414.2353 413.2280
619 414.2354 413.2281
620 414.2362 413.2289
621 418.7231 417.7158
622 418.7232 417.7159
623 421.2055 420.1982
624 421.2062 420.1989
625 421.2063 420.1990
626 421.2065 420.1992
627 421.2067 420.1994
628 421.2069 420.1996
629 421.2074 420.2001
630 421.2074 420.2001
631 421.2075 420.2002
632 421.2077 420.2004
633 421.2079 420.2006
634 423.1988 422.1915
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