MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
335 351.2186 350.2113
336 351.2202 350.2129
337 351.2380 350.2307
338 351.2380 350.2307
339 351.2380 350.2307
340 351.2382 350.2309
341 351.2383 350.2310
342 351.2384 350.2311
343 351.2390 350.2317
344 352.2025 351.1952
345 352.2026 351.1953
346 352.2035 351.1962
347 353.1776 352.1703
348 353.1787 352.1714
349 353.1788 352.1715
350 353.1793 352.1720
351 353.1794 352.1721
352 353.1799 352.1726
353 353.1801 352.1728
354 353.1813 352.1740
355 353.1815 352.1742
356 353.2527 352.2454
357 353.2539 352.2466
358 353.2540 352.2467
359 353.2542 352.2469
360 353.2551 352.2478
361 354.2390 353.2317
362 355.1931 354.1858
363 355.1944 354.1871
364 355.1945 354.1872
365 355.1948 354.1875
366 355.1948 354.1875
367 355.1953 354.1880
368 355.1956 354.1883
369 355.1962 354.1889
370 355.1975 354.1902
371 358.1847 357.1774
372 359.1930 358.1857
373 361.1719 360.1646
374 361.1720 360.1647
375 361.1841 360.1768
376 361.1844 360.1771
377 363.1800 362.1727
378 363.1809 362.1736
379 363.1814 362.1741
380 363.1815 362.1742
381 363.1818 362.1745
382 363.1819 362.1746
383 363.1822 362.1749
384 363.1837 362.1764
385 363.2009 362.1936
386 365.1819 364.1746
387 365.1820 364.1747
388 365.1957 364.1884
389 365.1971 364.1898
390 365.1971 364.1898
391 365.1973 364.1900
392 365.1974 364.1901
393 365.1977 364.1904
394 365.1979 364.1906
395 365.1980 364.1907
396 365.1981 364.1908
397 365.1985 364.1912
398 365.1988 364.1915
399 365.1998 364.1925
400 365.2155 364.2082
401 365.2165 364.2092
402 365.2167 364.2094
403 365.2171 364.2098
404 365.2176 364.2103
405 365.2186 364.2113
406 365.2186 364.2113
407 365.2197 364.2124
408 367.1927 366.1854
409 367.1938 366.1865
410 367.1940 366.1867
411 367.1942 366.1869
412 367.1944 366.1871
413 367.1949 366.1876
414 367.1956 366.1883
415 367.1971 366.1898
416 367.2113 366.2040
417 367.2127 366.2054
418 367.2131 366.2058
419 367.2133 366.2060
420 367.2133 366.2060
421 367.2135 366.2062
422 367.2139 366.2066
423 367.2144 366.2071
424 367.2323 366.2250
425 367.2332 366.2259
426 367.2332 366.2259
427 367.2333 366.2260
428 367.2339 366.2266
429 367.2362 366.2289
430 369.2103 368.2030
431 369.2105 368.2032
432 369.2108 368.2035
433 369.2110 368.2037
434 369.2271 368.2198
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