MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1245_Other_P1_B10.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,780

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4764)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
861 618.3314 617.3241 617.3207 5.58 0 19 0.099 +1Score > 23 indicates identity
Score > 21 indicates homology		 MGGLPK + Oxidation (M)
1619 478.2519 954.4892 954.4811 8.57 1 18 0.075 +1Score > 20 indicates identity FKFEDAAK
2279 1105.5751 1104.5678 1104.5676 0.21 1 17 0.11 +1Score > 23 indicates identity
Score > 20 indicates homology		 RDGPASFSLR
4220 797.4355 2389.2847 2389.3104 -10.8 0 17 0.079 +1Score > 18 indicates identity LQVHLLENNNDLDLTIGLLLK + 2 Deamidated (NQ)
1254 845.4373 844.4300 844.4402 -12.1 1 17 0.17 +1Score > 22 indicates identity NARENIK + Deamidated (NQ)
1867 508.7574 1015.5002 1015.4934 6.74 0 16 0.16 +1Score > 21 indicates identity
Score > 21 indicates homology		 GLNNGENVAK + Deamidated (NQ)
1447 911.4614 910.4541 910.4509 3.59 0 16 0.13 +1Score > 20 indicates identity GQNPVDGPK
1039 366.2088 730.4030 730.4014 2.32 0 16 0.31 +1Score > 23 indicates identity LGNIWK + Deamidated (NQ)
2801 659.3288 1316.6430 1316.6581 -11.4 0 16 0.059 +1Score > 22 indicates identity
Score > 16 indicates homology		 INIGKPLCNCK + Deamidated (NQ)
1106 385.1974 768.3802 768.3878 -9.85 1 15 0.16 +1Score > 20 indicates identity GNKANHK + Deamidated (NQ)
779 515.3312 514.3239 514.3227 2.34 0 15 0.3 +1Score > 22 indicates identity AVAVR
999 352.2036 702.3926 702.3912 2.08 0 15 0.44 +1Score > 25 indicates identity
Score > 24 indicates homology		 VASLADK
906 324.1721 646.3296 646.3286 1.65 0 15 0.45 +1Score > 24 indicates identity
Score > 24 indicates homology		 LSGNGAK + Deamidated (NQ)
1255 423.2226 844.4306 844.4403 -11.4 1 15 0.17 +1Score > 22 indicates identity
Score > 19 indicates homology		 EGGREGLK
1088 380.2045 758.3944 758.3922 2.91 0 14 0.11 +1Score > 21 indicates identity
Score > 17 indicates homology		 QQLNEK
1148 397.2113 792.4080 792.4051 3.69 0 14 0.12 +1Score > 23 indicates identity
Score > 18 indicates homology		 LSMVEAK + Oxidation (M)
1013 357.2136 712.4126 712.4119 1.01 0 14 0.12 +1Score > 18 indicates identity QDILPK
1355 442.2690 882.5234 882.5175 6.79 0 14 0.065 +1Score > 15 indicates identity VLPELQGK
1142 786.4717 785.4644 785.4759 -14.6 1 14 0.062 +1Score > 22 indicates identity
Score > 14 indicates homology		 KANLVNK
2007 523.7789 1045.5432 1045.5291 13.5 0 13 0.31 +1Score > 23 indicates identity
Score > 21 indicates homology		 ISILQNNNK + 3 Deamidated (NQ)
1179 403.6988 805.3830 805.3752 9.73 0 13 0.13 +1Score > 21 indicates identity
Score > 17 indicates homology		 MANSLDR
3778 688.9882 2063.9428 2063.9243 8.96 1 13 0.35 +1Score > 21 indicates identity RSNSDLQAQTASQSQSPEK + 3 Deamidated (NQ)
2982 734.8572 1467.6998 1467.6994 0.32 1 13 0.12 +1Score > 22 indicates identity
Score > 16 indicates homology		 AIPNNSFRDSFAK + 2 Deamidated (NQ)
2187 542.7865 1083.5584 1083.5673 -8.16 0 13 0.27 +1Score > 20 indicates identity LVGSDVGQGPR
2340 1122.5085 1121.5012 1121.5209 -17.5 0 13 0.12 +1Score > 21 indicates identity
Score > 16 indicates homology		 AGGEGMCIAIK + Oxidation (M)
1721 984.4682 983.4609 983.4784 -17.8 0 13 0.28 +1Score > 20 indicates identity NPSQRPQR + 2 Deamidated (NQ)
862 619.3464 618.3391 618.3337 8.81 0 12 0.13 +1Score > 23 indicates identity
Score > 16 indicates homology		 SNGTLK
926 329.1832 656.3518 656.3605 -13.3 0 12 0.18 +1Score > 17 indicates identity LNAPSR
994 701.3582 700.3509 700.3504 0.79 0 12 0.21 +1Score > 17 indicates identity INPSNR + Deamidated (NQ)
963 341.1880 680.3614 680.3493 17.8 1 12 0.32 +1Score > 19 indicates identity KNGSFK + Deamidated (NQ)
2069 529.7986 1057.5826 1057.5880 -5.03 1 12 0.66 +1Score > 22 indicates identity SRNIIQGIR + 2 Deamidated (NQ)
2954 724.3835 1446.7524 1446.7579 -3.78 0 11 0.73 +1Score > 22 indicates identity LGATIDVEHSHLR
809 556.3209 555.3136 555.3241 -18.9 1 11 0.13 +1Score > 15 indicates identity ARGPR
2428 573.2847 1144.5548 1144.5724 -15.3 0 11 0.41 +1Score > 22 indicates identity
Score > 20 indicates homology		 ENGLLNEISR + Deamidated (NQ)
2510 581.7922 1161.5698 1161.5513 15.9 0 11 0.11 +1Score > 23 indicates identity
Score > 14 indicates homology		 GLLGDSENETK
2873 695.3854 1388.7562 1388.7585 -1.59 1 11 0.52 +1Score > 21 indicates identity IGLAQLKLQMEK + 2 Deamidated (NQ); Oxidation (M)
2955 724.3838 1446.7530 1446.7579 -3.37 0 11 0.75 +1Score > 22 indicates identity LGATIDVEHSHLR
2933 723.8672 1445.7198 1445.7085 7.83 0 11 0.63 +1Score > 22 indicates identity SHGAVLPSYCSLR
2466 1152.5311 1151.5238 1151.5207 2.71 1 11 0.46 +1Score > 21 indicates identity
Score > 20 indicates homology		 KNVEDDGYGR
2251 550.2744 1098.5342 1098.5557 -19.5 0 11 0.45 +1Score > 20 indicates identity AELENLDAPK
1238 421.2263 840.4380 840.4341 4.68 0 11 0.37 +1Score > 19 indicates identity NATPVNPK + Deamidated (NQ)
2733 626.8156 1251.6166 1251.6064 8.22 1 11 0.1 +1Score > 24 indicates identity
Score > 14 indicates homology		 MSLKQMQSQR + Oxidation (M)
2813 664.8293 1327.6440 1327.6408 2.45 0 11 0.15 +1Score > 21 indicates identity
Score > 15 indicates homology		 NSATILNYFER + Deamidated (NQ)
1554 470.7647 939.5148 939.5099 5.25 0 11 0.36 -1Score > 19 indicates identity MIPELPPK + Oxidation (M)
2892 704.3691 1406.7236 1406.7252 -1.13 1 10 0.18 +1Score > 22 indicates identity
Score > 15 indicates homology		 SKALESEIASSSAK
1592 475.2599 948.5052 948.5029 2.51 1 10 0.72 +1Score > 22 indicates identity GPRTLYDK
2956 724.3863 1446.7580 1446.7579 0.089 0 10 0.8 +1Score > 22 indicates identity
Score > 22 indicates homology		 LGATIDVEHSHLR
4736 1145.0789 4576.2865 4576.2582 6.17 1 10 0.25 +1Score > 17 indicates identity LLEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNAR + Deamidated (NQ)
2768 639.3297 1276.6448 1276.6636 -14.7 1 10 0.97 +1Score > 23 indicates identity SPTNNNPRIHK
995 351.1833 700.3520 700.3504 2.39 0 10 0.35 +1Score > 18 indicates identity INPSNR + Deamidated (NQ)
1823 503.7701 1005.5256 1005.5165 9.13 1 10 1 +1Score > 23 indicates identity
Score > 23 indicates homology		 MKAGDDILK + Oxidation (M)
1429 906.4333 905.4260 905.4277 -1.80 0 10 0.28 +1Score > 23 indicates identity
Score > 17 indicates homology		 AVPSSSACK
922 329.1821 656.3496 656.3605 -16.6 0 10 0.23 +1Score > 16 indicates identity LNAPSR
871 313.1875 624.3604 624.3707 -16.5 1 10 0.37 +1Score > 18 indicates identity VKHNK
1055 371.1992 740.3838 740.3817 2.92 0 10 0.33 +1Score > 18 indicates identity KPPQDR + Deamidated (NQ)
1149 397.2278 792.4410 792.4381 3.67 0 10 0.42 +1Score > 21 indicates identity
Score > 19 indicates homology		 LSFEIGK
860 309.6663 617.3180 617.3207 -4.26 0 10 0.82 +1Score > 23 indicates identity
Score > 22 indicates homology		 MGGLPK + Oxidation (M)
2429 573.2847 1144.5548 1144.5724 -15.3 0 10 0.29 +1Score > 22 indicates identity
Score > 17 indicates homology		 ENGLLNEISR + Deamidated (NQ)
3907 1061.4888 2120.9630 2120.9473 7.41 0 10 0.22 +1Score > 21 indicates identity
Score > 16 indicates homology		 ENGMLATFGIGTNFTNDFR + Deamidated (NQ); Oxidation (M)
783 528.3149 527.3076 527.3067 1.66 0 10 0.19 +1Score > 15 indicates identity QGVPK
927 331.1616 660.3086 660.3191 -15.8 0 10 0.66 +1Score > 22 indicates identity
Score > 20 indicates homology		 ADAAGTR
2723 623.3365 1244.6584 1244.6360 18.0 1 10 0.15 +1Score > 22 indicates identity
Score > 14 indicates homology		 DNNLLGNKTQK + Deamidated (NQ)
1864 508.2667 1014.5188 1014.5094 9.31 0 10 0.4 +1Score > 21 indicates identity
Score > 18 indicates homology		 GLNNGENVAK
1131 783.4006 782.3933 782.3819 14.6 0 10 0.58 +1Score > 21 indicates identity
Score > 20 indicates homology		 AGMFMVK
1532 468.2391 934.4636 934.4760 -13.2 0 9 0.92 +1Score > 22 indicates identity NPNTFTLK + Deamidated (NQ)
3009 497.9104 1490.7094 1490.7327 -15.6 0 9 0.81 +1Score > 22 indicates identity
Score > 21 indicates homology		 LTNEDPPGLMYLK + Deamidated (NQ)
4028 1104.0491 2206.0836 2206.0708 5.82 0 9 1 +1Score > 22 indicates identity
Score > 22 indicates homology		 DKPYFDAEHVIHISHGWR
3510 926.4235 1850.8324 1850.8396 -3.89 1 9 0.7 +1Score > 20 indicates identity QGLEGNSYKFLFGDMK + 2 Deamidated (NQ); Oxidation (M)
925 657.3580 656.3507 656.3493 2.12 0 9 0.28 +1Score > 16 indicates identity VQPEGK
2968 730.3582 1458.7018 1458.6965 3.64 1 9 1 +1Score > 22 indicates identity
Score > 22 indicates homology		 HNMAKIYAGFYK + Deamidated (NQ); Oxidation (M)
3095 769.8602 1537.7058 1537.7341 -18.4 1 9 0.53 +1Score > 21 indicates identity
Score > 19 indicates homology		 TNSRMLLMNGNVR + Deamidated (NQ); 2 Oxidation (M)
1630 960.5312 959.5239 959.5287 -5.00 1 9 0.86 +1Score > 22 indicates identity
Score > 21 indicates homology		 NKIELTNK + Deamidated (NQ)
3008 745.8602 1489.7058 1489.6783 18.5 0 9 0.19 +1Score > 22 indicates identity
Score > 14 indicates homology		 AVEALNDSELNGEK + 2 Deamidated (NQ)
1550 470.2366 938.4586 938.4709 -13.1 0 9 0.69 +1Score > 20 indicates identity
Score > 20 indicates homology		 NVTDYLSK
1125 781.3943 780.3870 780.3766 13.4 0 9 0.57 +1Score > 21 indicates identity
Score > 19 indicates homology		 PAPQDPR + Deamidated (NQ)
2201 1088.5403 1087.5330 1087.5397 -6.11 0 9 0.65 +1Score > 22 indicates identity
Score > 19 indicates homology		 LNGQLNLEGK + 3 Deamidated (NQ)
2792 653.8455 1305.6764 1305.7003 -18.2 0 9 0.38 +1Score > 23 indicates identity
Score > 17 indicates homology		 MVASTTVPYLPK
878 630.3257 629.3184 629.3133 8.18 0 9 0.26 +1Score > 15 indicates identity TAQPGR + Deamidated (NQ)
1101 384.2062 766.3978 766.3861 15.3 0 9 0.59 +1Score > 20 indicates identity
Score > 19 indicates homology		 QLNYTK + Deamidated (NQ)
2943 723.8755 1445.7364 1445.7085 19.3 0 9 1 +1Score > 22 indicates identity
Score > 21 indicates homology		 SHGAVLPSYCSLR
1007 710.3005 709.2932 709.3031 -13.9 0 9 0.34 +1Score > 16 indicates identity FSGEDR
2503 1160.5629 1159.5556 1159.5770 -18.4 0 9 0.98 +1Score > 21 indicates identity MVPTMFPPPK + Oxidation (M)
1001 705.3210 704.3137 704.3089 6.81 0 9 0.54 +1Score > 18 indicates identity GNSGVDR + Deamidated (NQ)
826 584.3403 583.3330 583.3329 0.13 0 8 0.14 +1Score > 13 indicates identity APATPK
2267 1103.5052 1102.4979 1102.5142 -14.8 0 8 0.64 +1Score > 19 indicates identity EGGETQEKPK + Deamidated (NQ)
1406 899.4943 898.4870 898.4984 -12.7 1 8 0.55 +1Score > 18 indicates identity ERLQPTR
856 616.3676 615.3603 615.3592 1.89 0 8 1 +1Score > 25 indicates identity
Score > 21 indicates homology		 AVIEGK
1382 446.7359 891.4572 891.4596 -2.67 1 8 0.18 +1Score > 23 indicates identity
Score > 13 indicates homology		 LGMRGQSK + Oxidation (M)
2894 705.8547 1409.6948 1409.7006 -4.11 1 8 0.56 +1Score > 21 indicates identity
Score > 18 indicates homology		 NTNISIMRLGMK + Deamidated (NQ); 2 Oxidation (M)
2275 552.8080 1103.6014 1103.5863 13.7 0 8 0.94 +1Score > 22 indicates identity
Score > 20 indicates homology		 IPTVTFSDPK
2802 659.3477 1316.6808 1316.6646 12.4 1 8 1 +1Score > 23 indicates identity
Score > 21 indicates homology		 LKDGPGMDILNK + Deamidated (NQ); Oxidation (M)
1075 751.3747 750.3674 750.3773 -13.1 0 8 0.96 +1Score > 22 indicates identity
Score > 20 indicates homology		 QNHPQK
2893 705.8546 1409.6946 1409.7006 -4.25 1 8 0.27 +1Score > 21 indicates identity
Score > 15 indicates homology		 NTNISIMRLGMK + Deamidated (NQ); 2 Oxidation (M)
1417 903.4351 902.4278 902.4208 7.79 0 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 LSDFFMK + Oxidation (M)
1841 505.2721 1008.5296 1008.5314 -1.72 1 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 MVDKIFEK
3220 810.9358 1619.8570 1619.8631 -3.73 1 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 GKKPETGLLSNSFSR
858 618.3250 617.3177 617.3207 -4.79 0 8 0.74 +1Score > 23 indicates identity
Score > 19 indicates homology		 MGGLPK + Oxidation (M)
2058 1056.5345 1055.5272 1055.5400 -12.1 1 8 0.4 +1Score > 20 indicates identity
Score > 16 indicates homology		 RSGTFFNVK + Deamidated (NQ)
1658 967.4907 966.4834 966.5022 -19.4 0 8 0.38 +1Score > 22 indicates identity
Score > 16 indicates homology		 QLDTYISK
1103 767.4061 766.3988 766.3973 1.96 0 8 0.67 +1Score > 20 indicates identity
Score > 18 indicates homology		 KPEQHK + Deamidated (NQ)
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